The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Specific Tropism of HIV-1 for Microglial Cells in Primary Human Brain Cultures
Abstract
Human immunodeficiency virus (HIV) frequently causes neurological dysfunction and is abundantly expressed in the central nervous system (CNS) of acquired immunodeficiency syndrome (AIDS) patients with HIV encephalitis or myelopathy. The virus is found mostly in cells of the monocyte-macrophage lineage within the CNS, but the possibility of infection of other glial cells has been raised. Therefore, the effects of different HIV-1 and HIV-2 strains were studied in primary cultures of adult human brain containing microglial cells, the resident CNS macrophages, and astrocytes. These cultures could be productively infected with macrophage-adapted HIV-1 isolates but not with T lymphocyte-adapted HIV-1 isolates or two HIV-2 isolates. As determined with a triple-label procedure, primary astrocytes did not express HIV gag antigens and remained normal throughout the 3-week course of infection. In contrast, virus replicated in neighboring microglial cells, often leading to their cell fusion and death. The death of microglial cells, which normally serve immune functions in the CNS, may be a key factor in the pathogenesis of AIDS encephalitis or myelopathy.
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Cites
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Dual Infection of the Central Nervous System by AIDS Viruses with Distinct Cellular Tropisms
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Cited by
Microglia-specific localisation of a novel calcium binding protein, Iba1
Ito, D., Imai, Y., Ohsawa, K., Nakajima, K., Fukuuchi, Y., & Kohsaka, S. (1998) · Molecular Brain Research · doi:10.1016/s0169-328x(98)00040-0 · Cited by 1,476
CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia
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Sedgwick, J. D., Schwender, S., Imrich, H., Dörries, R., Butcher, G. W., & ter Meulen, V (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.16.7438 · Cited by 698
Human immunodeficiency virus infection of cells arrested in the cell cycle.
Lewis, P., Hensel, M., & Emerman, M. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05376.x · Cited by 531
Inhibition of Entry of HIV-1 in Neural Cell Lines by Antibodies Against Galactosyl Ceramide
Harouse, J. M., Bhat, S., Spitalnik, S. L., Laughlin, M., Stefano, K., Silberberg, D. H., & Gonzalez-Scarano, F. (1991) · Science · doi:10.1126/science.1857969 · Cited by 521
Metadata
- Authors
- Watkins, B. A., Dorn, H. H., Kelly, W. B., Armstrong, R. C., Potts, A. J., Michaels, F., Kufta, C. V., & Dubois-Dalcq, M.
- Venue
- Science
- Published
- 1990-08-03
- Volume
- 249
- Issue
- 4968
- Pages
- 549-553
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2053053546
- Cited by
- 342
- References
- 46
- FWCI
- 9.40
- Type
- journal article
- Language
- en
- Open access
- closed
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- Watkins, B. A., Dorn, H. H., Kelly, W. B., Armstrong, R. C., Potts, A. J., Michaels, F., Kufta, C. V., & Dubois-Dalcq, M. (1990). Specific Tropism of HIV-1 for Microglial Cells in Primary Human Brain Cultures. Science. https://doi.org/10.1126/science.2200125
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
