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Molecular Cloning of the Bombyx mori Prothoracicotropic Hormone
Abstract
Prothoracicotropic hormone (PTTH), a brain secretory polypeptide of insects, stimulates the prothoracic glands to produce and release ecdysone, the steroid essential to insect development. The complementary DNAs encoding PTTH of the silkmoth Bombyx mori were cloned and characterized, and the complete amino acid sequence was deduced. The data indicated that PTTH is first synthesized as a 224-amino acid polypeptide precursor containing three proteolytic cleavage signals. The carboxyl-terminal component (109 amino acids) that follows the last cleavage signal represents one PTTH subunit. Two PTTH subunits are linked together by disulfide bonds, before or after cleavage from prepro-PTTH, to form a homodimeric PTTH. When introduced into Escherichia coli cells, the complementary DNA directed the expression of an active substance that was functionally indistinguishable from natural PTTH. In situ hybridization showed the localization of the prepro-PTTH mRNA to two dorsolateral neurosecretory cells of the Bombyx brain.
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Cited by
Riddiford, L. M., Hiruma, K., Zhou, X., & Nelson, C. A. (2003) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2003.06.001 · Cited by 547
Control and biochemical nature of the ecdysteroidogenic pathway
Gilbert, L. I., Rybczynski, R., & Warren, J. T. (2002) · Annual Review of Entomology · doi:10.1146/annurev.ento.47.091201.145302 · Cited by 532
Prothoracicotropic Hormone Regulates Developmental Timing and Body Size in Drosophila
McBrayer, Z., Ono, H., Shimell, M., Parvy, J.-P., Beckstead, R. B., Warren, J. T., Thummel, C. S., Dauphin-Villemant, C., Gilbert, L. I., & O'Connor, M. B. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.11.003 · Cited by 434
Regulation of ecdysone production in Drosophila by neuropeptides and peptide hormones
Kannangara, J. R., Mirth, C. K., & Warr, C. G. (2021) · Open Biology · doi:10.1098/rsob.200373 · Cited by 84
Genome-guided transcript assembly by integrative analysis of RNA sequence data
Boley, N., Stoiber, M. H., Booth, B. W., Wan, K. H., Hoskins, R. A., Bickel, P. J., Celniker, S. E., & Brown, J. B. (2014) · Nature Biotechnology · doi:10.1038/nbt.2850 · Cited by 48
Metadata
- Authors
- Kawakami, A., Kataoka, H., Oka, T., Mizoguchi, A., Kimura-Kawakami, M., Adachi, T., Iwami, M., Nagasawa, H., Suzuki, A., & Ishizaki, H.
- Venue
- Science
- Published
- 1990-03-16
- Volume
- 247
- Issue
- 4948
- Pages
- 1333-1335
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2086970687
- Cited by
- 229
- References
- 0
- FWCI
- 3.69
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kawakami, A., Kataoka, H., Oka, T., Mizoguchi, A., Kimura-Kawakami, M., Adachi, T., Iwami, M., Nagasawa, H., Suzuki, A., & Ishizaki, H. (1990). Molecular Cloning of the Bombyx mori Prothoracicotropic Hormone. Science. https://doi.org/10.1126/science.2315701
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