The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Polymerase Chain Reaction with Single-Sided Specificity: Analysis of T Cell Receptor δ Chain
Abstract
In the polymerase chain reaction (PCR), two specific oligonucleotide primers are used to amplify the sequences between them. However, this technique is not suitable for amplifying genes that encode molecules where the 5′ portion of the sequences of interest is not known, such as the T cell receptor (TCR) or immunoglobulins. Because of this limitation, a novel technique, anchored polymerase chain reaction (A-PCR), was devised that requires sequence specificity only on the 3′ end of the target fragment. It was used to analyze TCR δ chain mRNA's from human peripheral blood γδ T cells. Most of these cells had a Vδ gene segment not previously described (V δ 3), and the δ chain junctional sequences formed a discrete subpopulation compared with those previously reported.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Cited by
Liu, Y.-G., & Whittier, R. F. (1995) · Genomics · doi:10.1016/0888-7543(95)80010-j · Cited by 1,123
Molecular cloning, expression and regional distribution of rat ciliary neurotrophic factor
Stöckli, K. A., Lottspeich, F., Sendtner, M., Masiakowski, P., Carroll, P., Götz, R., Lindholm, D., & Thoenen, H. (1989) · Nature · doi:10.1038/342920a0 · Cited by 616
DNA polymerase fidelity and the polymerase chain reaction.
Eckert, K. A., & Kunkel, T. A. (1991) · Genome Research · doi:10.1101/gr.1.1.17 · Cited by 448
Zelent, A., Mendelsohn, C., Kastner, P., Krust, A., Garnier, J. M., Ruffenach, F., Leroy, P., & Chambon, P. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb07922.x · Cited by 379
Gum, J. R., Hicks, J. W., Toribara, N. W., Rothe, E. M., Lagace, R. E., & Kim, Y. S. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)36620-7 · Cited by 273
Metadata
- Authors
- Loh, E. Y., Elliott, J. F., Cwirla, S., Lanier, L. L., & Davis, M. M.
- Venue
- Science
- Published
- 1989-01-13
- Volume
- 243
- Issue
- 4888
- Pages
- 217-220
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2021673452
- Cited by
- 629
- References
- 1
- FWCI
- 39.00
- Type
- journal article
- Language
- en
- Open access
- closed
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- Loh, E. Y., Elliott, J. F., Cwirla, S., Lanier, L. L., & Davis, M. M. (1989). Polymerase Chain Reaction with Single-Sided Specificity: Analysis of T Cell Receptor δ Chain. Science. https://doi.org/10.1126/science.2463672
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
