The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
The human MUC2 intestinal mucin has cysteine-rich subdomains located both upstream and downstream of its central repetitive region.
Open accessAbstract
The human MUC2 mucin is a large secretory glycoconjugate that coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Previous work has shown that this mucin contains an extended tandem repeat-containing domain rich in Thr and Pro. In the present work we describe two additional regions of this mucin located both upstream and downstream of the tandem repeat array. The carboxyl-terminal domain contains 984 residues and can be divided into mucin-like (139 residues) and cysteine-rich (845 residues) subdomains. This latter subdomain exhibits varying degrees of sequence similarity to a wide range of mucins and mucin-like proteins including those isolated from rats, pigs, cows, and frogs. We also report here the sequence of 1270 residues lying immediately upstream of the tandem repeats. This region contains a repetitive, mucin-like subdomain and a second cysteine-rich stretch of more than 700 residues. Both cysteine-rich subdomains of this mucin have sequence similarity with von Willebrand factor, a serum protein that exists as a disulfide-linked polymer. This suggests that these cysteine-rich subdomains are important in the catenation of mucin monomers into oligomers, the structures that confer viscoelasticity upon mucus.
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The gastrointestinal mucus system in health and disease
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Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin
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Self-citationFunction of the CysD domain of the gel-forming MUC2 mucin
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Metadata
- Authors
- Gum, J. R., Hicks, J. W., Toribara, N. W., Rothe, E. M., Lagace, R. E., & Kim, Y. S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1992-10
- Volume
- 267
- Issue
- 30
- Pages
- 21375-21383
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1586051439
- Cited by
- 273
- References
- 38
- FWCI
- 6.49
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Gum, J. R., Hicks, J. W., Toribara, N. W., Rothe, E. M., Lagace, R. E., & Kim, Y. S. (1992). The human MUC2 intestinal mucin has cysteine-rich subdomains located both upstream and downstream of its central repetitive region. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)36620-7
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