The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Mutant Potassium Channels with Altered Binding of Charybdotoxin, a Pore-Blocking Peptide Inhibitor
Abstract
The inhibition by charybdotoxin of A-type potassium channels expressed in Xenopus oocytes was studied for several splicing variants of the Drosophila Shaker gene and for several site-directed mutants of this channel. Charybdotoxin blocking affinity is lowered by a factor of 3.5 upon replacing glutamate-422 with glutamine, and by a factor of about 12 upon substituting lysine in this position. Replacement of glutamate-422 by aspartate had no effect on toxin affinity. Thus, the glutamate residue at position 422 of this potassium channel is near or in the externally facing mouth of the potassium conduction pathway, and the positively charged toxin is electrostatically focused toward its blocking site by the negative potential set up by glutamate-422.
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Cites
[19] Rapid and efficient site-specific mutagenesis without phenotypic selection
Kunkel, T. A., Roberts, J. D., & Zakour, R. A. (1987) · Methods in Enzymology · doi:10.1016/0076-6879(87)54085-x · Cited by 5,261
Structure and Function of Voltage-Sensitive Ion Channels
Catterall, W. A. (1988) · Science · doi:10.1126/science.2459775 · Cited by 1,174
Papazian, D. M., Schwarz, T. L., Tempel, B. L., Jan, Y. N., & Jan, L. Y. (1987) · Science · doi:10.1126/science.2441470 · Cited by 721
Sequence of a Probable Potassium Channel Component Encoded at Shaker Locus of Drosophila
Tempel, B. L., Papazian, D. M., Schwarz, T. L., Jan, Y. N., & Jan, L. Y. (1987) · Science · doi:10.1126/science.2441471 · Cited by 652
A transient calcium‐dependent chloride current in the immature Xenopus oocyte.
Barish, M. E. (1983) · The Journal of Physiology · doi:10.1113/jphysiol.1983.sp014852 · Cited by 621
Electrostatic recognition between superoxide and copper, zinc superoxide dismutase
Getzoff, E. D., Tainer, J. A., Weiner, P. K., Kollman, P. A., Richardson, J. S., & Richardson, D. C. (1983) · Nature · doi:10.1038/306287a0 · Cited by 554
Schwarz, T. L., Tempel, B. L., Papazian, D. M., Jan, Y. N., & Jan, L. Y. (1988) · Nature · doi:10.1038/331137a0 · Cited by 495
Shaker encodes a family of putative potassium channel proteins in the nervous system of Drosophila.
Pongs, O., Kecskemethy, N., Müller, R., Krah-Jentgens, I., Baumann, A., Kiltz, H. H., Canal, I., Llamazares, S., & Ferrus, A. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb02917.x · Cited by 437
Molecular characterization of Shaker, a Drosophila gene that encodes a potassium channel
Kamb, A., Iverson, L. E., & Tanouye, M. A. (1987) · Cell · doi:10.1016/0092-8674(87)90494-6 · Cited by 412
Mechanism of charybdotoxin block of the high-conductance, Ca2+-activated K+ channel.
MacKinnon, R., & Miller, C. (1988) · The Journal of general physiology · doi:10.1085/jgp.91.3.335 · Cited by 362
Self-citation
Cited by
P-type calcium channels in rat central and peripheral neurons
Mintz, I. M., Adams, M. E., & Bean, B. P. (1992) · Neuron · doi:10.1016/0896-6273(92)90223-z · Cited by 786
Tseng-Crank, J., Foster, C. D., Krause, J. D., Mertz, R., Godinot, N., DiChiara, T. J., & Reinhart, P. H. (1994) · Neuron · doi:10.1016/0896-6273(94)90418-9 · Cited by 428
Voltage-Gated Ion Channels and Electrical Excitability
Armstrong, C. M., & Hille, B. (1998) · Neuron · doi:10.1016/s0896-6273(00)80981-2 · Cited by 403
The charybdotoxin family of K+ channel-blocking peptides
Miller, C. (1995) · Neuron · doi:10.1016/0896-6273(95)90057-8 · Cited by 347
Self-citationGarcia-Calvo, M., Leonard, R. J., Novick, J., Stevens, S. P., Schmalhofer, W., Kaczorowski, G. J., & Garcia, M. L. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)46707-x · Cited by 345
Metadata
- Authors
- MacKinnon, R., & Miller, C.
- Venue
- Science
- Published
- 1989-09-22
- Volume
- 245
- Issue
- 4924
- Pages
- 1382-1385
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1972116208
- Cited by
- 347
- References
- 22
- FWCI
- 8.31
- Type
- journal article
- Language
- en
- Open access
- closed
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- MacKinnon, R., & Miller, C. (1989). Mutant Potassium Channels with Altered Binding of Charybdotoxin, a Pore-Blocking Peptide Inhibitor. Science. https://doi.org/10.1126/science.2476850
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
