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Effect of Carboxylic Acid Side Chains on the Absorption Maximum of Visual Pigments
Abstract
The proposal that the absorption maximum of the visual pigments is governed by interaction of the 11- cis -retinal chromophore with charged carboxylic acid side chains in the membrane-embedded regions of the proteins has been tested by mutating five Asp and Glu residues thought to be buried in rhodopsin. Changing Glu 113 to Gln causes a dramatic shift in the absorption maximum from 500 nanometers to 380 nanometers, a decrease in the p K a (acidity constant) of the protonated Schiff base of the chromophore to about 6, and a greatly increased reactivity with hydroxylamine. Thus Glu 113 appears to be the counterion to the protonated Schiff base. Wavelength modulation in visual pigments apparently is not governed by electrostatic interaction with carboxylate residues, other than the counterion.
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Cited by
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Metadata
- Authors
- Zhukovsky, E. A., & Oprian, D. D.
- Venue
- Science
- Published
- 1989-11-17
- Volume
- 246
- Issue
- 4932
- Pages
- 928-930
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2066769462
- Cited by
- 477
- References
- 0
- FWCI
- 6.37
- Type
- journal article
- Language
- en
- Open access
- closed
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