Evidence That Spt6p Controls Chromatin Structure by a Direct Interaction with Histones
Abstract
Genetic analysis has implicated SPT6 , an essential gene of Saccharomyces cerevisiae , in the control of chromatin structure. Mutations in SPT6 and particular mutations in histone genes are able to overcome transcriptional defects in strains lacking the Snf/Swi protein complex. Here it is shown that an spt6 mutation causes changes in chromatin structure in vivo. In addition, both in vivo and in vitro experiments provide evidence that Spt6p interacts directly with histones and primarily with histone H3. Consistent with these findings, Spt6p is capable of nucleosome assembly in vitro.
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Spt6 physically interacts with histone H3 and can facilitate nucleosome reassembly.
“Another factor reported to increase RNA Pol II speed is the transcription elongation factor Spt6, which physically interacts with histone H3 (Bortvin & Winston, 1996; Winkler et al, 2000) and can facilitate nucleosome reassembly (Adkins & Tyler, 2006).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Bortvin & Winston, 1996
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- Authors
- Bortvin, A., & Winston, F.
- Affiliations
- Harvard University (US)
- Corresponding author
- Winston, Fred
- Venue
- Science
- Published
- 1996-06-07
- Volume
- 272
- Issue
- 5267
- Pages
- 1473-1476
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1976561164
- Cited by
- 354
- References
- 36
- Citations by year
- 2025: 7 · 2024: 6 · 2023: 11 · 2022: 14 · 2021: 12 · 2020: 7 · 2019: 9 · 2018: 16 · 2017: 11 · 2016: 8 · 2015: 8 · 2014: 11 · 2013: 15 · 2012: 8
- FWCI
- 5.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM 32967)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- chromatin structure, histone H3, histone interaction, nucleosome assembly, transcriptional defects, Saccharomyces cerevisiae, histone genes
- MeSH terms
- Chromatin, DNA, Fungal, Fungal Proteins, Histones, Humans, Mutation, Nuclear Proteins, Nucleosomes, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Transcription, Genetic, Saccharomyces cerevisiae Proteins, Transcriptional Elongation Factors, Histone Chaperones
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