Cited by 354Genomics and Chromatin DynamicsChromatin Remodeling and CancerNuclear Structure and Function

Evidence That Spt6p Controls Chromatin Structure by a Direct Interaction with Histones

Bortvin, Alex, Winston, Fred·1996·Science

Abstract

Genetic analysis has implicated SPT6 , an essential gene of Saccharomyces cerevisiae , in the control of chromatin structure. Mutations in SPT6 and particular mutations in histone genes are able to overcome transcriptional defects in strains lacking the Snf/Swi protein complex. Here it is shown that an spt6 mutation causes changes in chromatin structure in vivo. In addition, both in vivo and in vitro experiments provide evidence that Spt6p interacts directly with histones and primarily with histone H3. Consistent with these findings, Spt6p is capable of nucleosome assembly in vitro.

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Crossref · 10.1126/science.272.5267.1473

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  • Spt6 physically interacts with histone H3 and can facilitate nucleosome reassembly.

    “Another factor reported to increase RNA Pol II speed is the transcription elongation factor Spt6, which physically interacts with histone H3 (Bortvin & Winston, 1996; Winkler et al, 2000) and can facilitate nucleosome reassembly (Adkins & Tyler, 2006).”

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Authors
Bortvin, A., & Winston, F.
Affiliations
Harvard University (US)
Corresponding author
Winston, Fred
Venue
Science
Published
1996-06-07
Volume
272
Issue
5267
Pages
1473-1476
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W1976561164
Cited by
354
References
36
Citations by year
2025: 7 · 2024: 6 · 2023: 11 · 2022: 14 · 2021: 12 · 2020: 7 · 2019: 9 · 2018: 16 · 2017: 11 · 2016: 8 · 2015: 8 · 2014: 11 · 2013: 15 · 2012: 8
FWCI
5.93
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (GM 32967)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
chromatin structure, histone H3, histone interaction, nucleosome assembly, transcriptional defects, Saccharomyces cerevisiae, histone genes
MeSH terms
Chromatin, DNA, Fungal, Fungal Proteins, Histones, Humans, Mutation, Nuclear Proteins, Nucleosomes, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Transcription, Genetic, Saccharomyces cerevisiae Proteins, Transcriptional Elongation Factors, Histone Chaperones

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