The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
A General Strategy for Selecting High-Affinity Zinc Finger Proteins for Diverse DNA Target Sites
Abstract
A method is described for selecting DNA-binding proteins that recognize desired sequences. The protocol involves gradually extending a new zinc finger protein across the desired 9- or 10-base pair target site, adding and optimizing one finger at a time. This procedure was tested with a TATA box, a p53 binding site, and a nuclear receptor element, and proteins were obtained that bind with nanomolar dissociation constants and discriminate effectively (greater than 20,000-fold) against nonspecific DNA. This strategy may provide important information about protein-DNA recognition as well as powerful tools for biomedical research.
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Citations
Cites
Crystal Structure of a Five-Finger GLI-DNA Complex: New Perspectives on Zinc Fingers
Pavletich, N. P., & Pabo, C. O. (1993) · Science · doi:10.1126/science.8378770 · Cited by 669
Self-citationIn vivo repression by a site-specific DNA-binding protein designed against an oncogenic sequence
Choo, Y., Sánchez-García, I., & Klug, A. (1994) · Nature · doi:10.1038/372642a0 · Cited by 292
Structure-Based Design of Transcription Factors
Pomerantz, J. L., Sharp, P. A., & Pabo, C. O. (1995) · Science · doi:10.1126/science.7809612 · Cited by 226
Self-citation
Cited by
Genome editing with engineered zinc finger nucleases
Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S., & Gregory, P. D. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2842 · Cited by 2,285
TALENs: A widely applicable technology for targeted genome editing
Joung, J. K., & Sander, J. D. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3486 · Cited by 1,650
DNA Recognition by Cys2His2 Zinc Finger Proteins
Wolfe, S. A., Nekludova, L., & Pabo, C. O. (2000) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.29.1.183 · Cited by 1,053
Self-citationAn improved zinc-finger nuclease architecture for highly specific genome editing
Miller, J. C., Holmes, M. C., Wang, J., Guschin, D. Y., Lee, Y.-L., Rupniewski, I., Beausejour, C. M., Waite, A. J., Wang, N. S., Kim, K. A., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1319 · Cited by 1,051
Self-citationTargeted Chromosomal Cleavage and Mutagenesis in Drosophila Using Zinc-Finger Nucleases
Bibikova, M., Golic, M., Golic, K. G., & Carroll, D. (2002) · Genetics · doi:10.1093/genetics/161.3.1169 · Cited by 860
Metadata
- Authors
- Greisman, H. A., & Pabo, C. O.
- Venue
- Science
- Published
- 1997-01-31
- Volume
- 275
- Issue
- 5300
- Pages
- 657-661
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W4246341361
- Cited by
- 331
- References
- 3
- FWCI
- 6.97
- Type
- journal article
- Language
- en
- Open access
- closed
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- Greisman, H. A., & Pabo, C. O. (1997). A General Strategy for Selecting High-Affinity Zinc Finger Proteins for Diverse DNA Target Sites. Science. https://doi.org/10.1126/science.275.5300.657
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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