The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Phosphorylation and Activation of p70 s6k by PDK1
Abstract
Activation of the protein p70 s6k by mitogens leads to increased translation of a family of messenger RNAs that encode essential components of the protein synthetic apparatus. Activation of the kinase requires hierarchical phosphorylation at multiple sites, culminating in the phosphorylation of the threonine in position 229 (Thr 229 ), in the catalytic domain. The homologous site in protein kinase B (PKB), Thr 308 , has been shown to be phosphorylated by the phosphoinositide-dependent protein kinase PDK1. A regulatory link between p70 s6k and PKB was demonstrated, as PDK1 was found to selectively phosphorylate p70 s6k at Thr 229 . More importantly, PDK1 activated p70 s6k in vitro and in vivo, whereas the catalytically inactive PDK1 blocked insulin-induced activation of p70 s6k .
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“mTOR phosphorylates p70S6 kinase (p70S6K) at several residues, resulting in increased kinase activity (31).”
From Mislocalization of the Cell Polarity Protein Scribble Promotes Mammary Tumorigenesis and Is Associated with Basal Breast Cancer (2014) · cites this paper as [31]
Citations
Cites
Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B
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Self-citationAlessi, D. R., James, S. R., Downes, C. P., Holmes, A. B., Gaffney, P. R. J., Reese, C. B., & Cohen, P. (1997) · Current Biology · doi:10.1016/s0960-9822(06)00122-9 · Cited by 2,874
Dual Role of Phosphatidylinositol-3,4,5-trisphosphate in the Activation of Protein Kinase B
Stokoe, D., Stephens, L. R., Copeland, T., Gaffney, P. R. J., Reese, C. B., Painter, G. F., Holmes, A. B., McCormick, F. P., & Hawkins, P. T. (1997) · Science · doi:10.1126/science.277.5325.567 · Cited by 1,216
Rapamycin suppresses 5′TOP mRNA translation through inhibition of p70s6k
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Self-citationAlessi, D. R., Deák, M., Casamayor, A., Caudwell, F. B., MORRICE, N. A., Norman, D. G., Gaffney, P. R. J., Reese, C. B., MacDougall, C. N., Harbison, D., et al. (1997) · Current Biology · doi:10.1016/s0960-9822(06)00336-8 · Cited by 754
PDGF- and insulin-dependent pp70S6k activation mediated by phosphatidylinositol-3-OH kinase
Chung, J., Grammar, T. C., Lemon, K. P., Kazlauskas, A., & Blenis, J. (1994) · Nature · doi:10.1038/370071a0 · Cited by 712
The modular phosphorylation and activation of p70 s6k
Pullen, N., & Thomas, G. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)00323-2 · Cited by 550
Self-citationAndjelković, M., Jakubowicz, T., Cron, P. D., Ming, X., Han, J. W., & Hemmings, B. A. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.12.5699 · Cited by 497
Self-citationPearson, R. B., Dennis, P. B., Han, J. W., Williamson, N. A., Kozma, S. C., Wettenhall, R. E., & Thomas, G. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb00212.x · Cited by 443
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Self-citationCharacterization of a Ca2+/Calmodulin-dependent Protein Kinase Cascade
Tokumitsu, H., Enslen, H., & Soderling, T. R. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.33.19320 · Cited by 229
Weng, Q. P., Andrabi, K. I., Klippel, A., Kozlowski, M. T., Williams, L. T., & Avruch, J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.12.5744 · Cited by 204
Dual Requirement for a Newly Identified Phosphorylation Site in p70 s6k
Moser, B. A., Dennis, P. B., Pullen, N., Pearson, R. B., Williamson, N. A., Wettenhall, R. E. H., Kozma, S. C., & Thomas, G. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.9.5648 · Cited by 103
Self-citation
Research works printed but not linked: 3
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- Ming X. F., et al., ibid. 371, 426 (1994).
- S. K. Hanks and T. Hunter in The Protein Kinase Facts Book: Protein-Serine Kinases G. Hardie and S. Hanks Eds. (Academic Press London 1995).
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Klionsky, D. J., & Emr, S. D. (2000) · Science · doi:10.1126/science.290.5497.1717 · Cited by 3,630
mTOR at the nexus of nutrition, growth, ageing and disease
Liu, G. Y., & Sabatini, D. M. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0199-y · Cited by 2,737
TOR, a Central Controller of Cell Growth
Schmelzle, T., & Hall, M. N. (2000) · Cell · doi:10.1016/s0092-8674(00)00117-3 · Cited by 1,914
The PI3K‒PDK1 connection: more than just a road to PKB
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Metadata
- Authors
- Pullen, N., Dennis, P. B., Andjelkovic, M., Dufner, A., Kozma, S. C., Hemmings, B. A., & Thomas, G.
- Affiliations
- Friedrich Miescher Institute, Maulbeerstrasse 66, CH-4058, Basel, Switzerland.
- Friedrich Miescher Institute (CH)
- Corresponding author
- Dennis, Patrick B.
- Hemmings, Brian A.
- Thomas, George
- Venue
- Science
- Published
- 1998-01-30
- Volume
- 279
- Issue
- 5351
- Pages
- 707-710
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2041926656
- Cited by
- 826
- References
- 13
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 13 · 2023: 11 · 2022: 14 · 2021: 20 · 2020: 12 · 2019: 12 · 2018: 10 · 2017: 13 · 2016: 12 · 2015: 21 · 2014: 22 · 2013: 29 · 2012: 29
- FWCI
- 28.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- p70S6K, PDK1, protein kinase A, protein kinase activation, protein synthesis regulation, insulin signaling, Akt, catalytic domain
- MeSH terms
- Wortmannin, Amino Acid Sequence, Androstadienes, Animals, Binding Sites, Catalysis, Cell Line, Enzyme Activation, Insulin, Insulin Antagonists, Molecular Sequence Data, Phosphorylation, Phosphothreonine, Polyenes, Proto-Oncogene Proteins, Recombinant Proteins, Protein Serine-Threonine Kinases, Calcium-Calmodulin-Dependent Protein Kinases, Ribosomal Protein S6 Kinases, Sirolimus, Proto-Oncogene Proteins c-akt, Calcium-Calmodulin-Dependent Protein Kinase Type 4, 3-Phosphoinositide-Dependent Protein Kinases
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