The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
An Essential Role for DNA Adenine Methylation in Bacterial Virulence
Abstract
Salmonella typhimurium lacking DNA adenine methylase (Dam) were fully proficient in colonization of mucosal sites but showed severe defects in colonization of deeper tissue sites. These Dam – mutants were totally avirulent and were effective as live vaccines against murine typhoid fever. Dam regulated the expression of at least 20 genes known to be induced during infection; a subset of these genes are among those activated by the PhoP global virulence regulator. PhoP, in turn, affected Dam methylation at specific genomic sites, as evidenced by alterations in DNA methylation patterns. Dam inhibitors are likely to have broad antimicrobial action, and Dam – derivatives of these pathogens may serve as live attenuated vaccines.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The Complete Genome Sequence of Escherichia coli K-12
Blattner, F. R., Plunkett, G., Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., et al. (1997) · Science · doi:10.1126/science.277.5331.1453 · Cited by 7,452
Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd
Fleischmann, R. D., Adams, M. D., White, O., Clayton, R. A., Kirkness, E. F., Kerlavage, A. R., Bult, C. J., Tomb, J.-F., Dougherty, B. A., Merrick, J. M., et al. (1995) · Science · doi:10.1126/science.7542800 · Cited by 5,654
Two-Component Signal Transduction
Stock, A. M., Robinson, V. L., & Goudreau, P. N. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.183 · Cited by 3,650
Complete Genome Sequence of Treponema pallidum, the Syphilis Spirochete
Fraser, C. M., Norris, S. J., Weinstock, G. M., White, O., Sutton, G. G., Dodson, R., Gwinn, M., Hickey, E. K., Clayton, R., Ketchum, K. A., et al. (1998) · Science · doi:10.1126/science.281.5375.375 · Cited by 1,081
Mg2+ as an Extracellular Signal: Environmental Regulation of Salmonella Virulence
Véscovi, E. G., Soncini, F. C., & Groisman, E. A. (1996) · Cell · doi:10.1016/s0092-8674(00)81003-x · Cited by 841
High Mutation Frequencies Among Escherichia coli and Salmonella Pathogens
LeClerc, J. E., Li, B., Payne, W. L., & Cebula, T. A. (1996) · Science · doi:10.1126/science.274.5290.1208 · Cited by 817
Selection of Bacterial Virulence Genes That Are Specifically Induced in Host Tissues
Mahan, M. J., Slauch, J. M., & Mekalanos, J. J. (1993) · Science · doi:10.1126/science.8430319 · Cited by 660
Self-citation[28] Purification, specific fragmentation, and separation of large DNA molecules
Smith, C. L., & Cantor, C. R. (1987) · Methods in Enzymology · doi:10.1016/0076-6879(87)55030-3 · Cited by 344
Molecular analysis of spv virulence genes of the salmonella virulence plasmids
Gulig, P. A., Danbara, H., Guiney, D. G., Lax, A. J., Norel, F., & Rhen, M. (1993) · Molecular Microbiology · doi:10.1111/j.1365-2958.1993.tb01172.x · Cited by 320
Bacterial infection as assessed by in vivo gene expression
Heithoff, D. M., Conner, C. P., Hanna, P. C., Julio, S. M., Hentschel, U., & Mahan, M. J. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.3.934 · Cited by 236
Self-citation
Research works printed but not linked: 9
- M. G. Marinus in Escherichia coli and Salmonella : Cellular and Molecular Biology F. Neidhardt Ed. (American Society for Microbiology Washington DC ed. 2 1996) pp. 782–791.
- J. A. Roberts et al. J. Urol. 133 1068 (1985). · doi:10.1016/s0022-5347(17)49382-7
- E. B. Newman R. T. Lin R. D'Ari in (1) pp. 1513–1525.
- Conner C. P., Heithoff D. M., Julio S. M., Sinsheimer R. L., Mahan M. J., ibid. 95, 4641 (1998).
- C. F. Earhart in (1) pp. 1075–1090.
- van der Woude M., Hale W. B., Low D. A., J. Bacteriol. 180, 5913 (1998). · doi:10.1128/jb.180.22.5913-5920.1998
- Hale W. B., van der Woude M. W., Low D. A., ibid. 176, 3438 (1994).
- Julio S. M., Conner C. P., Heithoff D. M., Mahan M. J., Mol. Gen. Genet. 258, 178 (1998). · doi:10.1007/s004380050721
- Slauch J. M., Silhavy T., ibid. 173, 4039 (1991).
Cited by
Wang, F., Lu, C.-H., & Willner, I. (2014) · Chemical Reviews · doi:10.1021/cr400354z · Cited by 649
THE PATHOGENESIS OF HELICOBACTER PYLORI–INDUCED GASTRO-DUODENAL DISEASES
Atherton, J. C. (2006) · Annual Review of Pathology: Mechanisms of Disease · doi:10.1146/annurev.pathol.1.110304.100125 · Cited by 567
Lee, K. A., Thomas, A. M., Bolte, L. A., Björk, J. R., de Ruijter, L. K., Armanini, F., Asnicar, F., Blanco-Miguez, A., Board, R., Calbet-Llopart, N., et al. (2022) · Nature Medicine · doi:10.1038/s41591-022-01695-5 · Cited by 464
Fang, G., Munera, D., Friedman, D. I., Mandlik, A., Chao, M. C., Banerjee, O., Feng, Z., Losic, B., Mahajan, M. C., Jabado, O. J., et al. (2012) · Nature Biotechnology · doi:10.1038/nbt.2432 · Cited by 360
Epigenetics of Host–Pathogen Interactions: The Road Ahead and the Road Behind
Gómez-Díaz, E., Jordà, M., Peinado, M. A., & Rivero, A. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1003007 · Cited by 269
Metadata
- Authors
- Heithoff, D. M., Sinsheimer, R. L., Low, D. A., & Mahan, M. J.
- Venue
- Science
- Published
- 1999-05-07
- Volume
- 284
- Issue
- 5416
- Pages
- 967-970
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2008243144
- Cited by
- 458
- References
- 18
- FWCI
- 14.86
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: An Essential Role for DNA Adenine Methylation in Bacterial Virulence. https://app.cochat.ai/papers/10.1126/science.284.5416.967
- The paper
- Heithoff, D. M., Sinsheimer, R. L., Low, D. A., & Mahan, M. J. (1999). An Essential Role for DNA Adenine Methylation in Bacterial Virulence. Science. https://doi.org/10.1126/science.284.5416.967
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
