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Cited by 213Ion channel regulation and functionIon Channels and ReceptorsErythrocyte Function and Pathophysiology

The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.

Molecular Identification of a Eukaryotic, Stretch-Activated Nonselective Cation Channel

Kanzaki, Makoto, Nagasawa, Masahiro, Kojima, Itaru, Sato, Chikara, Naruse, Keiji, Sokabe, Masahiro, Iida, Hidetoshi·1999·Science
DOI 10.1126/science.285.5429.882
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Abstract

Calcium-permeable, stretch-activated nonselective cation (SA Cat) channels mediate cellular responses to mechanical stimuli. However, genes encoding such channels have not been identified in eukaryotes. The yeast MID1 gene product (Mid1) is required for calcium influx in the yeast Saccharomyces cerevisiae . Functional expression of Mid1 in Chinese hamster ovary cells conferred sensitivity to mechanical stress that resulted in increases in both calcium conductance and the concentration of cytosolic free calcium. These increases were dependent on the presence of extracellular calcium and were reduced by gadolinium, a blocker of SA Cat channels. Single-channel analyses with cell-attached patches revealed that Mid1 acts as a calcium-permeable, cation-selective stretch-activated channel with a conductance of 32 picosiemens at 150 millimolar cesium chloride in the pipette. Thus, Mid1 appears to be a eukaryotic, SA Cat channel.

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Research works printed but not linked: 5

  1. ; F. Sachs in Sensory Transduction D. P. Corey and S. D. Roper Eds. (Rockefeller Univ. Press New York 1992) pp. 242–260;
  2. Iida H., Nakamura H., Ono T., Okumura M. S., Anraku Y., Mol. Cell. Biol. 14, 8259 (1994).
  3. Naruse K., Yamada T., Sokabe M., Am. J. Physiol. 274, H1532 (1998);
  4. ; L. Marsh and M. D. Rose in The Molecular and Cellular Biology of the Yeast Saccharomyces J. R. Pringle J. R. Broach E. W. Jones Eds. (Cold Spring Harbor Laboratory Press New York 1997) pp. 827–888.
  5. Supplementary materials are available at www.sciencemag.org/feature/data/1038393.shl

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Metadata

Authors
Kanzaki, M., Nagasawa, M., Kojima, I., Sato, C., Naruse, K., Sokabe, M., & Iida, H.
Affiliations
  • Gunma University (JP)
  • Supermolecular Science Division, Electrotechnical Laboratory, Tsukuba, Ibaraki 305-8568, Japan.
  • Nagoya University (JP)
  • Tokyo Gakugei University (JP)
Corresponding author
Iida, Hidetoshi
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
1999-08-06
Volume
285
Issue
5429
Pages
882-886
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.285.5429.882
OpenAlex
W2026402396
Cited by
213
References
23
Citations by year
2025: 2 · 2024: 3 · 2022: 4 · 2021: 4 · 2020: 5 · 2019: 2 · 2018: 4 · 2017: 6 · 2016: 5 · 2015: 7 · 2014: 9 · 2013: 9 · 2012: 9
FWCI
5.05
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
  • Health Sciences › Medicine › Physiology (medical) › Erythrocyte Function and Pathophysiology
Keywords
stretch-activated channels, non-selective cation channels, calcium-permeable channels, MID1, Saccharomyces cerevisiae, mechanical stress, calcium influx, gadolinium, single-channel conductance, cation selectivity, cytosolic free calcium
MeSH terms
Amino Acid Sequence, Animals, Calcium, Cations, Cell Membrane, Cell Membrane Permeability, Cesium, Chlorides, Fungal Proteins, Gadolinium, Cricetinae, Ion Channels, Membrane Glycoproteins, Membrane Potentials, Molecular Sequence Data, Pressure, Saccharomyces cerevisiae, Stress, Mechanical, Transfection, Calcium Channels, CHO Cells, Zinc Compounds, Patch-Clamp Techniques, Saccharomyces cerevisiae Proteins

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