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Extension of Cell Life-Span and Telomere Length in Animals Cloned from Senescent Somatic Cells
Abstract
The potential of cloning depends in part on whether the procedure can reverse cellular aging and restore somatic cells to a phenotypically youthful state. Here, we report the birth of six healthy cloned calves derived from populations of senescent donor somatic cells. Nuclear transfer extended the replicative life-span of senescent cells (zero to four population doublings remaining) to greater than 90 population doublings. Early population doubling level complementary DNA-1 (EPC-1, an age-dependent gene) expression in cells from the cloned animals was 3.5- to 5-fold higher than that in cells from age-matched (5 to 10 months old) controls. Southern blot and flow cytometric analyses indicated that the telomeres were also extended beyond those of newborn (<2 weeks old) and age-matched control animals. The ability to regenerate animals and cells may have important implications for medicine and the study of mammalian aging.
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“Somatic cell nuclear transfer of senescent fibroblasts from aged bovine donors results in cloned calves with fibroblasts that have restored population doubling and increased telomere maintenance compared to age-matched control animals180.”
From Stem cell aging: mechanisms, regulators and therapeutic opportunities (2014) · cites this paper as [180]
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Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
The serial cultivation of human diploid cell strains
Hayflick, L., & Moorhead, P. S. (1961) · Experimental Cell Research · doi:10.1016/0014-4827(61)90192-6 · Cited by 8,508
A biomarker that identifies senescent human cells in culture and in aging skin in vivo.
Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.20.9363 · Cited by 7,477
The limited in vitro lifetime of human diploid cell strains
Hayflick, L. (1965) · Experimental Cell Research · doi:10.1016/0014-4827(65)90211-9 · Cited by 5,876
Telomeres shorten during ageing of human fibroblasts
Harley, C. B., Futcher, A. B., & Greider, C. W. (1990) · Nature · doi:10.1038/345458a0 · Cited by 5,750
Viable offspring derived from fetal and adult mammalian cells
Wilmut, I., Schnieke, A., McWhir, J., Kind, A., & Campbell, K. (1997) · Nature · doi:10.1038/385810a0 · Cited by 4,976
Extension of Life-Span by Introduction of Telomerase into Normal Human Cells
Bodnar, A. G., Ouellette, M., Frolkis, M., Holt, S. E., Chiu, C.-P., Morin, G. B., Harley, C. B., Shay, J. W., Lichtsteiner, S., & Wright, W. E. (1998) · Science · doi:10.1126/science.279.5349.349 · Cited by 4,975
Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei
Wakayama, T., Perry, A. C. F., Zuccotti, M., Johnson, K. R., & Yanagimachi, R. (1998) · Nature · doi:10.1038/28615 · Cited by 2,250
Telomere length predicts replicative capacity of human fibroblasts.
Allsopp, R. C., Vaziri, H., Patterson, C., Goldstein, S., Younglai, E. V., Futcher, A. B., Greider, C. W., & Harley, C. B. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.21.10114 · Cited by 2,200
Cloned Transgenic Calves Produced from Nonquiescent Fetal Fibroblasts
Cibelli, J. B., Stice, S. L., Golueke, P. J., Kane, J. J., Jerry, J., Blackwell, C., de León, F. A. P., & Robl, J. M. (1998) · Science · doi:10.1126/science.280.5367.1256 · Cited by 1,396
Self-citationKiyono, T., Foster, S. A., Koop, J. I., McDougall, J. K., Galloway, D. A., & Klingelhutz, A. J. (1998) · Nature · doi:10.1038/23962 · Cited by 1,206
Telomere end-replication problem and cell aging
Levy, M. Z., Allsopp, R. C., Futcher, A. B., Greider, C. W., & Harley, C. B. (1992) · Journal of Molecular Biology · doi:10.1016/0022-2836(92)90096-3 · Cited by 1,069
Eight Calves Cloned from Somatic Cells of a Single Adult
Kato, Y., Tani, T., Sotomaru, Y., Kurokawa, K., Kato, J.-Y., Doguchi, H., Yasue, H., & Tsunoda, Y. (1998) · Science · doi:10.1126/science.282.5396.2095 · Cited by 1,055
Vaziri, H., & Benchimol, S. (1998) · Current Biology · doi:10.1016/s0960-9822(98)70109-5 · Cited by 1,003
Production of goats by somatic cell nuclear transfer
Baguisi, A. B., Behboodi, E., Melican, D. T., Pollock, J. S., Destrempes, M. M., Cammuso, C., Williams, J. L., Nims, S., Porter, C. A., Midura, P., et al. (1999) · Nature Biotechnology · doi:10.1038/8632 · Cited by 999
Control of Human Telomere Length by TRF1 and TRF2
Smogorzewska, A., van Steensel, B., Bianchi, A., Oelmann, S., Schaefer, M. R., Schnapp, G., & de Lange, T. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.5.1659-1668.2000 · Cited by 768
Rufer, N., Brümmendorf, T. H., Kolvraa, S., Bischoff, C., Christensen, K., Wadsworth, L., Schulzer, M., & Lansdorp, P. M. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.190.2.157 · Cited by 709
Self-citationTelomere length dynamics in human lymphocyte subpopulations measured by flow cytometry
Rufer, N., Dragowska, W. H., Thornbury, G., Roosnek, E. E., & Lansdorp, P. M. (1998) · Nature Biotechnology · doi:10.1038/nbt0898-743 · Cited by 551
Self-citationCellular senescence and DNA synthesis
Cristofalo, V. J., & Sharf, B. B. (1973) · Experimental Cell Research · doi:10.1016/0014-4827(73)90394-7 · Cited by 537
Self-citationSix cloned calves produced from adult fibroblast cells after long-term culture
Kubota, C., Yamakuchi, H., Todoroki, J., Mizoshita, K., Tabara, N., Barber, M. R., & Yang, X. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.3.990 · Cited by 487
Evidence for a Critical Telomere Length in Senescent Human Fibroblasts
Allsopp, R., & Harley, C. B. (1995) · Experimental Cell Research · doi:10.1006/excr.1995.1213 · Cited by 391
Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies)
Hill, J. R., Roussel, A. J., Cibelli, J. B., Edwards, J. F., Hooper, N. L., Miller, M. W., Thompson, J. A., Looney, C. R., Westhusin, M. E., Robl, J. M., et al. (1999) · Theriogenology · doi:10.1016/s0093-691x(99)00089-8 · Cited by 385
Self-citationVaziri, H., West, M. D., Allsopp, R., Davison, T. S., Wu, Y., Arrowsmith, C. H., Poirier, G. G., & Benchimol, S. (1997) · The EMBO Journal · doi:10.1093/emboj/16.19.6018 · Cited by 384
Self-citationAnalysis of telomere lengths in cloned sheep
Shiels, P. G., Kind, A., Campbell, K., Waddington, D., Wilmut, I., Colman, A., & Schnieke, A. (1999) · Nature · doi:10.1038/20580 · Cited by 350
Replicative senescence of human fibroblast-like cells in culture
Cristofalo, V. J., & Pignolo, R. J. (1993) · Physiological Reviews · doi:10.1152/physrev.1993.73.3.617 · Cited by 286
Self-citationLymphoid hypoplasia and somatic cloning
Renard, J. P. J. P., Chastant‐Maillard, S., Chesné, P., Richard, C., Marchal, J., Cordonnier, N., Chavatte‐Palmer, P., & Vignon, X. (1999) · The Lancet · doi:10.1016/s0140-6736(98)12173-6 · Cited by 254
West, M. D., Pereira‐Smith, O. M., & Smith, J. R. (1989) · Experimental Cell Research · doi:10.1016/0014-4827(89)90372-8 · Cited by 247
Self-citationRhesus Monkeys Produced by Nuclear Transfer1
Li, M., Ely, J. J., Stouffer, R. L., & Wolf, D. P. (1997) · Biology of Reproduction · doi:10.1095/biolreprod57.2.454 · Cited by 239
Ultrastructural changes accompanying the aging of human diploid cells in culture
Lipetz, J., & Cristofalo, V. J. (1972) · Journal of Ultrastructure Research · doi:10.1016/s0022-5320(72)80005-4 · Cited by 178
Self-citationLanza, R., Cibelli, J. B., & West, M. D. (1999) · Nature Medicine · doi:10.1038/12404 · Cited by 156
Self-citationThe Cellular and Molecular Biology of Skin Aging
West, M. D. (1994) · Archives of Dermatology · doi:10.1001/archderm.1994.01690010091014 · Cited by 155
Self-citationProspects for the use of nuclear transfer in human transplantation
Lanza, R., Cibelli, J. B., & West, M. D. (1999) · Nature Biotechnology · doi:10.1038/70709 · Cited by 143
Self-citationThe cellular and molecular biology of skin aging.
West, M. D. (1994) · PubMed · Cited by 130
Self-citationPignolo, R. J., Cristofalo, V. J., & Rotenberg, M. O. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)52964-1 · Cited by 126
Self-citationGalli, C., Duchi, R., Moor, R. M., & Lazzari, G. (1999) · Cloning · doi:10.1089/15204559950019924 · Cited by 117
West, M. D., Shay, J. W., Wright, W. E., & Linskens, M. H. K. (1996) · Experimental Gerontology · doi:10.1016/0531-5565(95)02013-6 · Cited by 94
Self-citationUnlimited Mileage from Telomerase?
de Lange, T., & DePinho, R. A. (1999) · Science · doi:10.1126/science.283.5404.947 · Cited by 72
Aging and Cellular Defense Mechanisms
Giacomoni, P. U. (1992) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1992.tb38642.x · Cited by 59
Molecular markers of senescence in fibroblast-like cultures
Cristofalo, V. J., & Pignolo, R. J. (1996) · Experimental Gerontology · doi:10.1016/0531-5565(95)02018-7 · Cited by 59
Self-citationPignolo, R. J., Martin, B. G., Horton, J. H., Kalbach, A., & Cristofalo, V. J. (1998) · Experimental Gerontology · doi:10.1016/s0531-5565(97)00090-9 · Cited by 46
Self-citationGorman, S. D., & Cristofalo, V. J. (1986) · Experimental Cell Research · doi:10.1016/0014-4827(86)90206-5 · Cited by 34
Self-citationMolecular Changes with in Vitro Cellular Senescence
Cristofalo, V. J., Pignolo, R. J., & Rotenberg, M. O. (1992) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1992.tb38662.x · Cited by 19
Self-citationPhinney, D. G., Keiper, C. L., Francis, M. K., & Ryder, K. (1994) · PubMed · Cited by 17
Self-citation
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1 of the 44 references couldn’t be matched to a known paper.
Research works printed but not linked: 2
- Chomczynski P., Sacchi N., Anal. Biochem. 162, 156 (1987). · DOI
- Phinney D. G., Keiper C. L., Francis M. K., Ryder K., Oncogene 9, 2353 (1994).
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Genomic imprinting: parental influence on the genome
Reik, W., & Walter, J. (2001) · Nature Reviews Genetics · doi:10.1038/35047554 · Cited by 2,288
Switching and Signaling at the Telomere
Blackburn, E. H. (2001) · Cell · doi:10.1016/s0092-8674(01)00492-5 · Cited by 2,139
Telomeres and human disease: ageing, cancer and beyond
Blasco, M. A. (2005) · Nature Reviews Genetics · doi:10.1038/nrg1656 · Cited by 1,434
Aubert, G., & Lansdorp, P. M. (2008) · Physiological Reviews · doi:10.1152/physrev.00026.2007 · Cited by 1,188
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Metadata
- Authors
- Lanza, R. P., Cibelli, J. B., Blackwell, C., Cristofalo, V. J., Francis, M. K., Baerlocher, G. M., Mak, J., Schertzer, M., Chavez, E. A., Sawyer, N., Lansdorp, P. M., & West, M. D.
- Affiliations
- Advanced Cell Technology (United States) (US)
- Thomas Jefferson University (US)
- Terry Fox Research Institute (CA)
- University of British Columbia (CA)
- Corresponding author
- Lanza, Robert P.
- ORCID
- Venue
- Science
- Published
- 2000-04-28
- Volume
- 288
- Issue
- 5466
- Pages
- 665-669
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2141926462
- Cited by
- 466
- References
- 44
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 10 · 2022: 8 · 2021: 10 · 2020: 4 · 2019: 9 · 2018: 3 · 2017: 7 · 2016: 7 · 2015: 7 · 2014: 13 · 2013: 16 · 2012: 16
- FWCI
- 34.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- ScholarWorks@UMassAmherst (University of Massachusetts Amherst) (submittedVersion)
- Funders
- National Institutes of Health (1r01gm056162-01)
- National Institute on Aging (AG00378)
- National Institute of General Medical Sciences (GM56162)
- National Institute of Allergy and Infectious Diseases (AI 29524)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- somatic cell nuclear transfer, cloning, cellular aging, replicative lifespan, telomere length, telomere elongation, cloned calves, age-related gene expression, regenerative medicine, mammalian aging
- MeSH terms
- Pigment Epithelium-Derived Factor, Animals, Cattle, Cell Division, Cells, Cultured, Clone Cells, Embryo Transfer, Eye Proteins, Female, Fibroblasts, Flow Cytometry, Longevity, Nerve Growth Factors, Proteins, RNA, Messenger, Blotting, Southern, Serpins, Matched-Pair Analysis, Telomere, Cellular Senescence, In Situ Hybridization, Fluorescence, DNA, Complementary, Cloning, Organism, Nuclear Transfer Techniques
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