The DOI matches its Crossref record; 43 of the 44 references it cites were found in a registry or an index.
Extension of Cell Life-Span and Telomere Length in Animals Cloned from Senescent Somatic Cells
Abstract
The potential of cloning depends in part on whether the procedure can reverse cellular aging and restore somatic cells to a phenotypically youthful state. Here, we report the birth of six healthy cloned calves derived from populations of senescent donor somatic cells. Nuclear transfer extended the replicative life-span of senescent cells (zero to four population doublings remaining) to greater than 90 population doublings. Early population doubling level complementary DNA-1 (EPC-1, an age-dependent gene) expression in cells from the cloned animals was 3.5- to 5-fold higher than that in cells from age-matched (5 to 10 months old) controls. Southern blot and flow cytometric analyses indicated that the telomeres were also extended beyond those of newborn (<2 weeks old) and age-matched control animals. The ability to regenerate animals and cells may have important implications for medicine and the study of mammalian aging.
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“Somatic cell nuclear transfer of senescent fibroblasts from aged bovine donors results in cloned calves with fibroblasts that have restored population doubling and increased telomere maintenance compared to age-matched control animals180.”
From Stem cell aging: mechanisms, regulators and therapeutic opportunities (2014) · cites this paper as [180]
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Self-citation
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Jaenisch, R., & Bird, A. (2003) · Nature Genetics · doi:10.1038/ng1089 · Cited by 6,325
Genomic imprinting: parental influence on the genome
Reik, W., & Walter, J. (2001) · Nature Reviews Genetics · doi:10.1038/35047554 · Cited by 2,288
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Blasco, M. A. (2005) · Nature Reviews Genetics · doi:10.1038/nrg1656 · Cited by 1,434
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Self-citation
Metadata
- Authors
- Lanza, R. P., Cibelli, J. B., Blackwell, C., Cristofalo, V. J., Francis, M. K., Baerlocher, G. M., Mak, J., Schertzer, M., Chavez, E. A., Sawyer, N., Lansdorp, P. M., & West, M. D.
- Affiliations
- Advanced Cell Technology (United States) (US)
- Thomas Jefferson University (US)
- Terry Fox Research Institute (CA)
- University of British Columbia (CA)
- Corresponding author
- Lanza, Robert P.
- ORCID
- Venue
- Science
- Published
- 2000-04-28
- Volume
- 288
- Issue
- 5466
- Pages
- 665-669
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2141926462
- Cited by
- 466
- References
- 44
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 10 · 2022: 8 · 2021: 10 · 2020: 4 · 2019: 9 · 2018: 3 · 2017: 7 · 2016: 7 · 2015: 7 · 2014: 13 · 2013: 16 · 2012: 16
- FWCI
- 34.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- ScholarWorks@UMassAmherst (University of Massachusetts Amherst) (submittedVersion)
- Funders
- National Institutes of Health (1r01gm056162-01)
- National Institute on Aging (AG00378)
- National Institute of General Medical Sciences (GM56162)
- National Institute of Allergy and Infectious Diseases (AI 29524)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- somatic cell nuclear transfer, cloning, cellular aging, replicative lifespan, telomere length, telomere elongation, cloned calves, age-related gene expression, regenerative medicine, mammalian aging
- MeSH terms
- Pigment Epithelium-Derived Factor, Animals, Cattle, Cell Division, Cells, Cultured, Clone Cells, Embryo Transfer, Eye Proteins, Female, Fibroblasts, Flow Cytometry, Longevity, Nerve Growth Factors, Proteins, RNA, Messenger, Blotting, Southern, Serpins, Matched-Pair Analysis, Telomere, Cellular Senescence, In Situ Hybridization, Fluorescence, DNA, Complementary, Cloning, Organism, Nuclear Transfer Techniques
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