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Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression
Abstract
Little is known about the DNA helicases required for the elongation phase of eukaryotic chromosome replication. Minichromosome maintenance (MCM) protein complexes have DNA helicase activity but have only been functionally implicated in initiating DNA replication. Using an improved method for constructing conditional degron mutants, we show that depletion of MCMs after initiation irreversibly blocks the progression of replication forks in Saccharomyces cerevisiae . Like the Escherichia coli dnaB and SV40 T antigen helicases, therefore, the MCM complex is loaded at origins before initiation and is essential for elongation. Restricting MCM loading to the G 1 phase ensures that initiation and elongation occur just once per cell cycle.
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Citations
Cited by
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Banaszynski, L. A., Chen, L.-C., Maynard-Smith, L. A., Ooi, A. G. L., & Wandless, T. J. (2006) · Cell · doi:10.1016/j.cell.2006.07.025 · Cited by 902
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Branzei, D., & Foiani, M. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2852 · Cited by 786
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Initiating DNA synthesis: from recruiting to activating the MCM complex
Lei, M., & Tye, B. K. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.8.1447 · Cited by 365
DNA replication as a target of the DNA damage checkpoint
Zegerman, P., & Diffley, J. F. X. (2009) · DNA Repair · doi:10.1016/j.dnarep.2009.04.023 · Cited by 128
Self-citationA conserved mechanism for regulating replisome disassembly in eukaryotes
Jenkyn-Bedford, M., Jones, M. L., Baris, Y., Labib, K. P. M., Cannone, G., Yeeles, J. T. P., & Deegan, T. D. (2021) · Nature · doi:10.1038/s41586-021-04145-3 · Cited by 116
Self-citationA Nontranscriptional Role for HIF-1α as a Direct Inhibitor of DNA Replication
Hubbi, M. E., Kshitiz, Gilkes, D. M., Rey, S., Wong, C. C., Luo, W., Kim, D.-H., Dang, C. V., Levchenko, A., & Semenza, G. L. (2013) · Science Signaling · doi:10.1126/scisignal.2003417 · Cited by 116
Perspectives on the DNA damage and replication checkpoint responses in Saccharomyces cerevisiae
Putnam, C. D., Jaehnig, E. J., & Kolodner, R. D. (2009) · DNA Repair · doi:10.1016/j.dnarep.2009.04.021 · Cited by 83
Ye, Y., Xiao, L., Wang, S.-J., Yue, W., Yin, Q.-S., Sun, M.-Y., Xia, W., Shao, Z.-Y., & Zhang, H. (2016) · Oncotarget · doi:10.18632/oncotarget.6473 · Cited by 47
Li, J., Liu, J., Liang, Z., He, F., Yang, L., Li, P., Jiang, Y., Wang, B., Zhou, C., Wang, Y., et al. (2017) · Cell Death & Disease · doi:10.1038/cddis.2017.46 · Cited by 43
Retracted
Metadata
- Authors
- Labib, K., Tercero, J. A., & Diffley, J. F. X.
- Venue
- Science
- Published
- 2000-06-02
- Volume
- 288
- Issue
- 5471
- Pages
- 1643-1647
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1968074226
- Cited by
- 625
- References
- 0
- FWCI
- 16.11
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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