The DOI matches its Crossref record.
Localized Rac Activation Dynamics Visualized in Living Cells
Abstract
Signaling proteins are thought to be tightly regulated spatially and temporally in order to generate specific and localized effects. For Rac and other small guanosine triphosphatases, binding to guanosine triphosphate leads to interaction with downstream targets and regulates subcellular localization. A method called FLAIR (fluorescence activation indicator for Rho proteins) was developed to quantify the spatio-temporal dynamics of the Rac1 nucleotide state in living cells. FLAIR revealed precise spatial control of growth factor–induced Rac activation, in membrane ruffles and in a gradient of activation at the leading edge of motile cells. FLAIR exemplifies a generally applicable approach for examining spatio-temporal control of protein activity.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
RHO GTPASES: Biochemistry and Biology
Jaffe, A. B., & Hall, A. (2005) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.21.020604.150721 · Cited by 3,009
A landscape of driver mutations in melanoma
Hodis, E., Watson, I. R., Kryukov, G. V., Arold, S. T., Imielinski, M., Theurillat, J.-P., Nickerson, E., Auclair, D., Li, L., Place, C., et al. (2012) · Cell · doi:10.1016/j.cell.2012.06.024 · Cited by 2,610
Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations
Sekar, R. B., & Periasamy, A. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200210140 · Cited by 932
Conserved microtubule–actin interactions in cell movement and morphogenesis
Rodriguez, O. C., Schaefer, A. W., Mandato, C. A., Forscher, P., Bement, W. M., & Waterman-Storer, C. M. (2003) · Nature Cell Biology · doi:10.1038/ncb0703-599 · Cited by 844
Imaging biochemistry inside cells
Wouters, F. S., Verveer, P. J., & Bastiaens, P. I. H. (2001) · Trends in Cell Biology · doi:10.1016/s0962-8924(01)01982-1 · Cited by 395
Metadata
- Authors
- Kraynov, V. S., Chamberlain, C., Bokoch, G. M., Schwartz, M. A., Slabaugh, S., & Hahn, K. M.
- Venue
- Science
- Published
- 2000-10-13
- Volume
- 290
- Issue
- 5490
- Pages
- 333-337
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1984387240
- Cited by
- 678
- References
- 0
- FWCI
- 16.00
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Localized Rac Activation Dynamics Visualized in Living Cells. https://app.cochat.ai/papers/10.1126/science.290.5490.333
- The paper
- Kraynov, V. S., Chamberlain, C., Bokoch, G. M., Schwartz, M. A., Slabaugh, S., & Hahn, K. M. (2000). Localized Rac Activation Dynamics Visualized in Living Cells. Science. https://doi.org/10.1126/science.290.5490.333
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
