The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Generation of Lymphohematopoietic Cells from Embryonic Stem Cells in Culture
Abstract
An efficient system was developed that induced the differentiation of embryonic stem (ES) cells into blood cells of erythroid, myeloid, and B cell lineages by coculture with the stromal cell line OP9. This cell line does not express functional macrophage colony-stimulating factor (M-CSF). The presence of M-CSF had inhibitory effects on the differentiation of ES cells to blood cells other than macrophages. Embryoid body formation or addition of exogenous growth factors was not required, and differentiation was highly reproducible even after the selection of ES cells with the antibiotic G418. Combined with the ability to genetically manipulate ES cells, this system will facilitate the study of molecular mechanisms involved in development and differentiation of hematopoietic cells.
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Cited by
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Regulation of B lymphocyte and macrophage development by graded expression of PU.1
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Metadata
- Authors
- Nakano, T., Kodama, H., & Honjo, T.
- Venue
- Science
- Published
- 1994-08-19
- Volume
- 265
- Issue
- 5175
- Pages
- 1098-1101
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2000927180
- Cited by
- 871
- References
- 25
- FWCI
- 5.15
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nakano, T., Kodama, H., & Honjo, T. (1994). Generation of Lymphohematopoietic Cells from Embryonic Stem Cells in Culture. Science. https://doi.org/10.1126/science.8066449
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
