The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Structure of DNA Polymerase I Klenow Fragment Bound to Duplex DNA
Abstract
Klenow fragment of Escherichia coli DNA polymerase I, which was cocrystallized with duplex DNA, positioned 11 base pairs of DNA in a groove that lies at right angles to the cleft that contains the polymerase active site and is adjacent to the 3′ to 5′ exonuclease domain. When the fragment bound DNA, a region previously referred to as the "disordered domain" became more ordered and moved along with two helices toward the 3′ to 5′ exonuclease domain to form the binding groove. A single-stranded, 3′ extension of three nucleotides bound to the 3′ to 5′ exonuclease active site. Although this cocrystal structure appears to be an editing complex, it suggests that the primer strand approaches the catalytic site of the polymerase from the direction of the 3′ to 5′ exonuclease domain and that the duplex DNA product may bend to enter the cleft that contains the polymerase catalytic site.
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Cites
Crystal structure at 3.5 A resolution of HIV-1 reverse transcriptase complexed with an inhibitor
Kohlstaedt, L. A., Wang, J., Friedman, J. M., Rice, P. A., & Steitz, T. A. (1992) · Science · doi:10.1126/science.1377403 · Cited by 1,914
Self-citationCrystal Structure of a CAP-DNA Complex: the DNA Is Bent by 90°
Schultz, S. C., Shields, G. C., & Steitz, T. A. (1991) · Science · doi:10.1126/science.1653449 · Cited by 1,102
Self-citationBeese, L. S., & Steitz, T. A. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb07917.x · Cited by 1,048
Self-citationStructure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP
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Self-citationDNA recognition by GAL4: structure of a protein-DNA complex
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An attempt to unify the structure of polymerases
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Enzymatic Synthesis of Deoxyribonucleic Acid
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Cocrystal structure of an editing complex of Klenow fragment with DNA.
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Self-citationDerbyshire, V., Grindley, N. D., & Joyce, C. M. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb07916.x · Cited by 272
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Cited by
Kunkel, T. A., & Bebenek, K. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.497 · Cited by 1,003
DNA Polymerases: Structural Diversity and Common Mechanisms
Steitz, T. A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.25.17395 · Cited by 848
Self-citationStructure of the Replicating Complex of a Pol α Family DNA Polymerase
Franklin, M. C., Wang, J., & Steitz, T. A. (2001) · Cell · doi:10.1016/s0092-8674(01)00367-1 · Cited by 538
Self-citationBrautigam, C. A., & Steitz, T. A. (1998) · Current Opinion in Structural Biology · doi:10.1016/s0959-440x(98)80010-9 · Cited by 377
Self-citationStructure of Taq polymerase with DNA at the polymerase active site
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Metadata
- Authors
- Beese, L. S., Derbyshire, V., & Steitz, T. A.
- Venue
- Science
- Published
- 1993-04-16
- Volume
- 260
- Issue
- 5106
- Pages
- 352-355
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2165639777
- Cited by
- 474
- References
- 23
- FWCI
- 32.67
- Type
- journal article
- Language
- en
- Open access
- closed
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