Blocking promiscuous activation at cryptic promoters directs cell type–specific gene expression
Open accessAbstract
Blocking somatic genes to make sperm: To generate cells with a specific identity, only a subset of genes is used. Most studies focus on factors that turn on cell type–specific gene expression. However, mechanisms are also needed to block expression of genes that specify other cell lineages. Kim et al. identified such a mechanism in the Drosophila male germ line. A multiple–zinc finger protein and a chromatin remodeler were found to act together to block transcription from cryptic promoters. These factors prevented aberrant gene expression and enabled proper differentiation in the adult sperm stem cell lineage. Science , this issue p. 717
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The repressor Kmg inhibits somatic lineage genes in Drosophila melanogaster germ cells.
“For example, the repressor Kmg inhibits somatic lineage genes in Drosophila melanogaster germ cells57.”
From Active repression of cell fate plasticity by PROX1 safeguards hepatocyte identity and prevents liver tumorigenesis (2025) · cites this paper as [57]
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Ermolaeva, M., Neri, F., Ori, A., & Rudolph, K. L. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0020-3 · Cited by 272 · see citing works
Canalizing cell fate by transcriptional repression
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- Authors
- Kim, J., Lu, C., Srinivasan, S., Awe, S., Brehm, A., & Fuller, M. T.
- Affiliations
- Stanford Medicine (US)
- Philipps University of Marburg (DE)
- Corresponding author
- Lu, Chenggang
- Srinivasan, Shrividhya
- Fuller, Margaret T.
- Venue
- Science
- Published
- 2017-05-19
- Volume
- 356
- Issue
- 6339
- Pages
- 717-721
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC5572561
- OpenAlex
- W2616432106
- Cited by
- 39
- References
- 2
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 6 · 2023: 5 · 2022: 3 · 2021: 2 · 2020: 1 · 2019: 4 · 2018: 8
- FWCI
- 1.45
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of General Medical Sciences (award300489, 5R01GM061986)
- Deutsche Forschungsgemeinschaft (award310176, TRR81)
- Reed-Hodgson Professorship in Human Biology (award300877)
- Stanford Graduate Fellowship (award300878)
- Bio-X Stanford Interdisciplinary Graduate Fellowship (award300879)
- Kempkes Stiftung (award310236)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- cryptic promoter, transcriptional repression, cell type-specific gene expression, Aly, spermatocyte differentiation, male germline stem cells, zinc finger proteins, terminal differentiation
- MeSH terms
- Adenosine Triphosphatases, Animals, Autoantigens, Cell Differentiation, Chromatin, Drosophila melanogaster, Female, Gene Expression Regulation, Male, Organ Specificity, Promoter Regions, Genetic, RNA, Messenger, Spermatocytes, Stem Cells, Testis, Cell Cycle Proteins, Cell Lineage, Drosophila Proteins
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