Spatiotemporal structure of cell fate decisions in murine neural crest
Abstract
Binary decisions refine fate decisions: Neural crest cells develop into tissues ranging from craniofacial bones to peripheral neurons. Combining single-cell RNA sequencing with spatial transcriptomics, Soldatov et al. analyzed how neural crest cells in mouse embryos decide among the various fates available to them (see the Perspective by Mayor). These multipotent cells become biased toward a given fate early on and step through a progression of binary decisions as their fate is refined. Competing fate programs coexist until increased synchronization favors one and repression disfavors the other. Science , this issue p. eaas9536 ; see also p. 937
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The mesenchymal group contained NR2F1+MEF2C+TWIST1+, consistent with CNCC-derived mesenchyme capable of generating dermal cells.
“The mesenchymal group contained NR2F1+MEF2C+TWIST1+, consistent with CNCC-derived mesenchyme capable of generating dermal cells (Fig. 2d, Extended Data Fig. 5)21.”
From Hair-bearing human skin generated entirely from pluripotent stem cells (2020) · cites this paper as [21]
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- Authors
- Soldatov, R., Kaucka, M., Kastriti, M. E., Petersen, J., Chontorotzea, T., Englmaier, L., Akkuratova, N., Yang, Y., Häring, M., Dyachuk, V., Bock, C., Farlik, M., Piacentino, M. L., Boismoreau, F., Hilscher, M. M., Yokota, C., Qian, X., Nilsson, M., Bronner, M. E., Croci, L., Hsiao, W.-Y., Guertin, D. A., Brunet, J.-F., Consalez, G. G., Ernfors, P., Fried, K., Kharchenko, P. V., & Adameyko, I.
- Affiliations
- Harvard University (US)
- Department of Molecular Neurosciences, Center for Brain Research, Medical University Vienna, 1090 Vienna, Austria.
- Karolinska Institutet (SE)
- Medical University of Vienna (AT)
- St Petersburg University (RU)
- Department of Neuroscience, Karolinska Institutet, 17177 Stockholm, Sweden.
- Austrian Academy of Sciences (AT)
- California Institute of Technology (US)
- Centre National de la Recherche Scientifique (FR)
- Stockholm University (SE)
- Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
- Vita-Salute San Raffaele University (IT)
- University of Massachusetts Chan Medical School (US)
- Institut de Biologie de l’ENS (IBENS), INSERM, CNRS, École Normale Supérieure, PSL Research University, 75005 Paris, France.
- Corresponding author
- Soldatov, Ruslan
- Kastriti, Maria Eleni
- Kharchenko, Peter V.
- Adameyko, Igor
- ORCID
- Soldatov, Ruslan
- Kaucka, Marketa
- Kastriti, Maria Eleni
- Petersen, Julian
- Chontorotzea, Tatiana
- Englmaier, Lukas
- Akkuratova, Natalia
- Yang, Yunshi
- Häring, Martin
- Dyachuk, Viacheslav
- Bock, Christoph
- Farlik, Matthias
- Piacentino, Michael L.
- Hilscher, Markus M.
- Yokota, Chika
- Qian, Xiaoyan
- Nilsson, Mats
- Bronner, Marianne E.
- Croci, Laura
- Guertin, David A.
- Brunet, Jean-Francois
- Consalez, Gian Giacomo
- Ernfors, Patrik
- Fried, Kaj
- Kharchenko, Peter V.
- Adameyko, Igor
- Venue
- Science
- Published
- 2019-06-07
- Volume
- 364
- Issue
- 6444
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2948517648
- Cited by
- 546
- References
- 9
- Citations by year
- 2026: 69 · 2025: 69 · 2024: 80 · 2023: 70 · 2022: 79 · 2021: 90 · 2020: 74 · 2019: 14 · 2013: 1
- FWCI
- 21.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- The Journal of the American Medical Association (JAMA) Network (American Medical Association) (submittedVersion)
- Archivio Istituzionale della Ricerca - Università Vita-Salute San Raffaele (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- NIH Office of the Director (Statistical Methods for analysis of Single Cell variability)
- NSF Office of the Director
- European Research Council (“STEMMING-FROM-NERVE” N647844)
- Knut och Alice Wallenbergs Stiftelse
- Vetenskapsrådet (2016-03645)
- Bertil Hållstens Forskningsstiftelse
- paradifference foundation
- Familjen Erling Perssons stiftelse
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- neural crest cells, cell fate determination, multipotent stem cells, craniofacial bone development, peripheral neurons, mouse embryo, spatiotemporal gene expression
- MeSH terms
- Animals, Mice, Mutant Strains, Nerve Tissue Proteins, Neural Crest, Neuroglia, Neurons, Nuclear Proteins, Gene Expression Regulation, Developmental, Cell Lineage, Mice, Basic Helix-Loop-Helix Transcription Factors, Basic Helix-Loop-Helix Proteins, Twist-Related Protein 1, Neural Tube, Neurogenesis, Neural Stem Cells, Single-Cell Analysis, Mesenchymal Stem Cells
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