The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Hematopoietic stem cell heterogeneity and age-associated platelet bias are evolutionarily conserved
Abstract
Hematopoietic stem cells (HSCs) reconstitute multilineage human hematopoiesis after clinical bone marrow (BM) transplantation and are the cells of origin of some hematological malignancies. Although HSCs provide multilineage engraftment, individual murine HSCs are lineage biased and contribute unequally to blood cell lineages. Here, we performed high-throughput single-cell RNA sequencing in mice after xenograft with molecularly barcoded adult human BM HSCs. We demonstrated that human individual BM HSCs are also functionally and transcriptionally lineage biased. Specifically, we identified platelet-biased and multilineage human HSCs. Quantitative comparison of transcriptomes from single HSCs from young and aged BM showed that both the proportion of platelet-biased HSCs and their level of transcriptional platelet priming increase with age. Therefore, platelet-biased HSCs and their increased prevalence and transcriptional platelet priming during aging are conserved features of mammalian evolution.
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“Together with recent transplantation studies of human HSCs50, this result suggests that there is conservation of the processes that drive haematopoietic ageing and decline in clonal complexity, and it highlights that CH mutations might not be the main driver of this process.”
From Clonal tracing with somatic epimutations reveals dynamics of blood ageing (2025) · cites this paper as [50]
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Human bone marrow hematopoietic stem cells are increased in frequency and myeloid-biased with age
Pang, W. W., Price, E. A., Sahoo, D., Beerman, I., Maloney, W. J., Rossi, D. J., Schrier, S. L., & Weissman, I. L. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1116110108 · Cited by 991
Orchestrating single-cell analysis with Bioconductor
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Densely Interconnected Transcriptional Circuits Control Cell States in Human Hematopoiesis
Novershtern, N., Subramanian, A., Lawton, L. N., Mak, R. H., Haining, W. N., McConkey, M. E., Habib, N., Yosef, N., Chang, C. Y., Shay, T., et al. (2011) · Cell · doi:10.1016/j.cell.2011.01.004 · Cited by 942
Lineage tracing on transcriptional landscapes links state to fate during differentiation
Weinreb, C., Rodriguez-Fraticelli, A., Camargo, F. D., & Klein, A. M. (2020) · Science · doi:10.1126/science.aaw3381 · Cited by 759 · cited 1×
“However, erythroid-biased clonal output has been observed after ex vivo barcoding and transplantation of murine HSPCs ( 26 ).”
BackgroundClonal analysis of lineage fate in native haematopoiesis
Rodriguez-Fraticelli, A. E., Wolock, S. L., Weinreb, C. S., Panero, R., Patel, S. H., Jankovic, M., Sun, J., Calogero, R. A., Klein, A. M., & Camargo, F. D. (2018) · Nature · doi:10.1038/nature25168 · Cited by 596
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Polylox barcoding reveals haematopoietic stem cell fates realized in vivo
Pei, W., Feyerabend, T. B., Rössler, J., Wang, X., Postrach, D., Busch, K., Rode, I., Klapproth, K., Dietlein, N., Quedenau, C., et al. (2017) · Nature · doi:10.1038/nature23653 · Cited by 443
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Self-citationA practical solution to pseudoreplication bias in single-cell studies
Zimmerman, K. D., Espeland, M. A., & Langefeld, C. D. (2021) · Nature Communications · doi:10.1038/s41467-021-21038-1 · Cited by 356
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Cited by
Clonal tracing with somatic epimutations reveals dynamics of blood ageing
Scherer, M., Singh, I., Braun, M. M., Szu-Tu, C., Sanchez Sanchez, P., Lindenhofer, D., Jakobsen, N. A., Körber, V., Kardorff, M., Nitsch, L., et al. (2025) · Nature · doi:10.1038/s41586-025-09041-8 · Cited by 89
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Metadata
- Authors
- Aksöz, M., Gafencu, G.-A., Stoilova, B., Buono, M., Zhang, Y., Turkalj, S., Meng, Y., Jakobsen, N. A., Metzner, M., Clark, S.-A., Beveridge, R., Thongjuea, S., Vyas, P., & Nerlov, C.
- Venue
- Science Immunology
- Published
- 2024-08-23
- Volume
- 9
- Issue
- 98
- ISSN
- 2470-9468
- Cited by
- 40
- References
- 10
- Language
- en
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