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Cited by 649Nanoparticle-Based Drug DeliveryBarrier Structure and Function StudiesAdvanced Drug Delivery Systems

A Dense Poly(Ethylene Glycol) Coating Improves Penetration of Large Polymeric Nanoparticles Within Brain Tissue

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Nance, Elizabeth A., Woodworth, Graeme F., Sailor, Kurt A., Shih, Ting-Yu, Xu, Qingguo, Swaminathan, Ganesh, Xiang, Dennis, Eberhart, Charles +1 more·2012·Science Translational Medicine
DOI 10.1126/scitranslmed.3003594PMC PMC3718558
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Abstract

Nanoparticles densely coated with poly(ethylene glycol) rapidly penetrate within mouse, rat, and human brain parenchyma.

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Crossref · 10.1126/scitranslmed.3003594

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  • In mice, nanoparticles densely coated with PEG measuring up to 200 nm in diameter were able to spread within brain tissue, a size range achievable for LNPs.

    “In mice, nanoparticles densely coated with PEG measuring up to 200 nm in diameter were able to spread within brain tissue (28), a size range achievable for LNPs (9, 29).”

    From Lipid Nanoparticles Deliver mRNA to the Brain after an Intracerebral Injection (2023) · cites this paper as [28]

    The Results section (‘Particle tracking in live mouse brain’; page not provided) reports that densely PEG-coated 40- and 100-nm particles penetrated mouse brain tissue, but 200-nm particles did not. The separate claim about sizes achievable for LNPs is attributed to other citations and is not judged here. (LLM, conf=0.94)

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  • PEGylated polymer nanoparticles similar in size and surface chemistry to LNPs were previously able to transfect DNA into brain tissue after an intracerebral injection, suggesting that LNPs will be able to transfect brain tissue after intracerebral injection.

    “Notably, PEGylated polymer nanoparticles have previously been able to transfect DNA into brain tissue after an intracerebral injection; these nanoparticles were similar in size and surface chemistry as LNPs, suggesting that LNPs will be able to transfect brain tissue after intracerebral injection (28, 30).”

    From Lipid Nanoparticles Deliver mRNA to the Brain after an Intracerebral Injection (2023) · cites this paper as [28]

    Split into 2 atoms — 0 confirmed, 2 partially supported, 0 disputed, 0 misattributed, 0 unverifiable. Expand the claim to see per-atom verdicts.

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  • Human brain extracellular space has pores even larger than 200 nm in diameter, although more than 95% of all pores have diameters of 175 nm or smaller.

    “In addition, a previous study estimated that human brain extracellular space has pores even larger than 200 nm in diameter, although more than 95% of all pores have diameters of 175 nm or smaller (28).”

    From Lipid Nanoparticles Deliver mRNA to the Brain after an Intracerebral Injection (2023) · cites this paper as [28]

    The Results section on particle tracking in human brain tissue ex vivo reports estimated ECS pores as large as 225 nm, supporting the claim that some exceed 200 nm. It reports approximately 28% larger than 100 nm, but does not state that more than 95% are 175 nm or smaller. No page number is available in our copy. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Cited by

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Authors
Nance, E. A., Woodworth, G. F., Sailor, K. A., Shih, T.-Y., Xu, Q., Swaminathan, G., Xiang, D., Eberhart, C., & Hanes, J.
Affiliations
Johns Hopkins University (US)
Corresponding author
  • Nance, Elizabeth A.
  • Woodworth, Graeme F.
  • Hanes, Justin
ORCID
Venue
Science Translational Medicine
Publisher
American Association for the Advancement of Science (AAAS)
Published
2012-08-29
Volume
4
Issue
149
Pages
149ra119-149ra119
ISSN
  • 1946-6234 (ISSN-L)
  • 1946-6242
DOI
10.1126/scitranslmed.3003594
PMC
PMC3718558
OpenAlex
W1976970322
Cited by
649
References
5
Citations by year
2026: 33 · 2025: 38 · 2024: 47 · 2023: 49 · 2022: 54 · 2021: 62 · 2020: 57 · 2019: 43 · 2018: 48 · 2017: 49 · 2016: 57 · 2015: 48 · 2014: 36 · 2013: 26 · 2012: 2
FWCI
14.52
Type
journal article
Language
en
Open access
Locations
  • Science Translational Medicine (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Eye Institute (T32EY017203)
  • National Cancer Institute (R01CA164789, T32CA130840, U54CA151838)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
Keywords
polyethylene glycol coating, brain extracellular space, nanoparticle size, pore size distribution, blood-brain barrier, biodegradable nanoparticles, brain drug delivery
MeSH terms
Animals, Blood-Brain Barrier, Brain, Chemistry, Pharmaceutical, Diffusion, Drug Carriers, Female, Humans, Microscopy, Fluorescence, Particle Size, Permeability, Polyethylene Glycols, Polystyrenes, Surface Properties, Time Factors, Rats, Sprague-Dawley, Paclitaxel, Microscopy, Confocal, Microscopy, Video, Coated Materials, Biocompatible, Nanotechnology, Mice, Rats, Nanoparticles

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