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Cited by 70Polymer Surface Interaction StudiesTissue Engineering and Regenerative MedicineHydrogels: synthesis, properties, applications

Gastrointestinal synthetic epithelial linings

Open access
Li, Junwei, Wang, Thomas, Kirtane, Ameya R., Shi, Yunhua, Jones, Alexis, Moussa, Zaina, Lopes, Aaron, Collins, Joy +8 more·2020·Science Translational Medicine
DOI 10.1126/scitranslmed.abc0441PMC PMC8221077
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Abstract

A targeted coating of polydopamine polymerized in situ in the small intestine enables drug delivery and modulation of nutrient absorption in pigs.

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Crossref · 10.1126/scitranslmed.abc0441

Cites

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  • Prolonged reaction times lead to a gradual increase in PDA coating thickness.

    “Similarly, prolonged reaction times lead to a gradual increase in coating thickness.”

    From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [16]

    The Results section, “Endogenous enzyme-catalyzed PDA growth on epithelium” (page unavailable), reports that the PDA coating signal increased over time but reached completion within 12 minutes. It measures coating amount by signal, not thickness, and does not establish a gradual thickness increase with prolonged reaction times. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

  • Higher pH values promote faster dopamine polymerization and result in the formation of thicker PDA coatings.

    “In general, higher pH values promote faster polymerization and result in the formation of thicker PDA coatings.”

    From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [16]

    The Results section on endogenous enzyme-catalyzed PDA growth reports faster polymerization with catalase, not with higher pH. A reference title mentions studying PDA deposition as a function of pH, but our copy gives no finding that higher pH speeds polymerization or produces thicker coatings (searched: higher pH and pH). (LLM, conf=0.76)

    Experimental — accuracy not yet measured

  • Increasing dopamine monomer concentration accelerates polymerization, but excessively high concentrations induce abundant suspended PDA aggregates that compromise coating uniformity and surface quality.

    “Increasing the dopamine monomer concentration can further accelerate polymerization; however, excessively high concentrations may induce the formation of abundant suspended PDA aggregates in the solution, thereby compromising coating uniformity and surface quality.”

    From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [16]

    The Results section on endogenous enzyme-catalyzed PDA growth reports faster polymerization with catalase while dopamine was maintained at the same concentration. Searches for concentration-dependent acceleration and suspended aggregates found no passage stating that higher dopamine concentrations accelerate polymerization or impair coating uniformity and surface quality. (LLM, conf=0.76)

    Experimental — accuracy not yet measured

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Cites

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Research works printed but not linked: 55

  1. [2]Peterson LW, Artis D, Intestinal epithelial cells: regulators of barrier function and immune homeostasis, Nat. Rev. Immunol. 14, 141– 153 (2014). 24566914
  2. [3]Anselmo AC, Gokarn Y, Mitragotri S, Non-invasive delivery strategies for biologics, Nat. Rev. Drug Discov. 18, 19– 40 (2018). 30498202
  3. [4]Zelikin AN, Ehrhardt C, Healy AM, Materials and methods for delivery of biological drugs, Nat. Chem. 8, 997– 1007 (2016). 27768097
  4. [6]Lee Y, Deelman TE, Chen K, Lin DSY, Tavakkoli A, Karp JM, Therapeutic luminal coating of the intestine, Nat. Mater. 17, 834– 842 (2018). 29891893
  5. [7]Kitano K, Schwartz DM, Zhou H, Gilpin SE, Wojtkiewicz GR, Ren X, Sommer CA, Capilla AV, Mathisen DJ, Goldstein AM, Mostoslavsky G, Ott HC, Bioengineering of functional human induced pluripotent stem cell-derived intestinal grafts, Nat. Commun. 8, 765 (2017). 29018244 PMC5635127
  6. [8]Rajagopalan H, Cherrington AD, Thompson CC, Kaplan LM, Rubino F, Mingrone G, Becerra P, Rodriguez P, Vignolo P, Caplan J, Rodriguez L, Galvao Neto MP, Endoscopic duodenal mucosal resurfacing for the treatment of type 2 diabetes: 6-month interim analysis from the first-in-human proof-of-concept study., Diabetes Care 39, 2254– 2261 (2016). 27519448
  7. [9]Kaukinen K, Lindfors K, Mäki M, Advances in the treatment of coeliac disease: an immunopathogenic perspective, Nat. Rev. Gastroenterol. Hepatol. 11, 36– 44 (2014). 23917697
  8. [10]Torres J, Mehandru S, Colombel J-F, Peyrin-Biroulet L, Crohn’s disease, Lancet 389, 1741– 1755 (2017). 27914655
  9. [11]Green PHR, Cellier C, Celiac disease, N. Engl. J. Med 357, 1731– 1743 (2007). 17960014
  10. [12]Camaschella C, Iron-deficiency anemia, N. Engl. J. Med. 372, 1832– 1843 (2015). 25946282
  11. [14]Elloumi-Hannachi I, Yamato M, Okano T, Cell sheet engineering: a unique nanotechnology for scaffold-free tissue reconstruction with clinical applications in regenerative medicine, J. Intern. Med. 267, 54– 70 (2010). 20059644
  12. [15]Kirkegaard P, Christensen AB, Ibsen J, Hegedüs V, Christiansen J, Experimental nonsuture colonic anastomoses, Am. J. Surg. 139, 233– 236 (1980). 7356107
  13. [16]Fishbein TM, Intestinal transplantation, N. Engl. J. Med 361, 998– 1008 (2009). 19726774
  14. [17]Khademhosseini A, Langer R, A decade of progress in tissue engineering, Nat. Protoc. 11, 1775– 1781 (2016). 27583639
  15. [18]Odenwald MA, Turner JR, The intestinal epithelial barrier: a therapeutic target?, Nat. Rev. Gastroenterol. Hepatol. 14, 9– 21 (2017). 27848962 PMC5554468
  16. [19]Carino GP, Mathiowitz E, Oral insulin delivery, Adv. Drug Deliv. Rev. 35, 249– 257 (1999). 10837700
  17. [20]Thakral S, Thakral NK, Majumdar DK, Eudragit®: a technology evaluation, Expert Opin. Drug Deliv. 10, 131– 149 (2013). 23102011
  18. [21]Khan MZI, Prebeg Ž, Kurjaković N, A pH-dependent colon targeted oral drug delivery system using methacrylic acid copolymers. I. Manipulation of drug release using Eudragit® L100–55 and Eudragit® S100 combinations, J. Control. Release 58, 215– 222 (1999). 10053194
  19. [22]Laulicht B, Tripathi A, Mathiowitz E, Diuretic bioactivity optimization of furosemide in rats, Eur. J. Pharm. Biopharm. 79, 314– 319 (2011). 21571067
  20. [23]Abramson A, Caffarel-Salvador E, Soares V, Minahan D, Tian RY, Lu X, Dellal D, Gao Y, Kim S, Wainer J, Collins J, Tamang S, Hayward A, Yoshitake T, Lee HC, Fujimoto J, Fels J, Frederiksen MR, Rahbek U, Roxhed N, Langer R, Traverso G, A luminal unfolding microneedle injector for oral delivery of macromolecules, Nat. Med. 25, 1512– 1518 (2019). 31591601 PMC7218658
  21. [24]Harris FC, Pyloric stenosis: hold-up of enteric coated aspirin tablets, Br. J. Surg. 60, 979– 981 (1973). 4764749
  22. [25]Abuhelwa AY, Foster DJR, Upton RN, A quantitative review and meta-models of the variability and factors affecting oral drug absorption—part I: gastrointestinal pH, AAPS J. 18, 1309– 1321 (2016). 27495120
  23. [26]Dressman J, Krämer J, Pharmaceutical dissolution testing (Taylor & Francis, 2005).
  24. [27]Lee H, Dellatore SM, Miller WM, Messersmith PB, Mussel-inspired surface chemistry for multifunctional coatings., Science 318, 426– 30 (2007). 17947576 PMC2601629
  25. [28]Lee H, Rho J, Messersmith PB, Facile conjugation of biomolecules onto surfaces via mussel adhesive protein inspired coatings, Adv. Mater. 21, 431– 434 (2009). 19802352 PMC2755254
  26. [30]Ofikwu GI, Sarhan M, Ahmed L, EVICEL glue-induced small bowel obstruction after laparoscopic gastric bypass., Surg. Laparosc. Endosc. Percutan. Tech. 23, e38– 40 (2013). 23386172
  27. [32]Lomer MCE, Parkes GC, Sanderson JD, Review article: lactose intolerance in clinical practice-myths and realities, Aliment. Pharmacol. Ther. 27, 93– 103 (2007). 17956597
  28. [33]Storhaug CL, Fosse SK, Fadnes LT, Country, regional, and global estimates for lactose malabsorption in adults: a systematic review and meta-analysis, Lancet Gastroenterol. Hepatol. 2, 738– 746 (2017). 28690131
  29. [34]Rosado JL, Solomons NW, Lisker R, Bourges H, Anrubio G, García A, Perez-Briceño R, Aizupuru E, Enzyme replacement therapy for primary adult lactase deficiency: effective reduction of lactose malabsorption and milk intolerance by direct addition of β-galactosidase to milk at mealtime, Gastroenterology 87, 1072– 1082 (1984). 6434367
  30. [35]Leader B, Baca QJ, Golan DE, Protein therapeutics: a summary and pharmacological classification, Nat. Rev. Drug Discov. 7, 21– 39 (2008). 18097458
  31. [38]Lin Z, Forster J, Sarosiek I, McCallum RW, Treatment of diabetic gastroparesis by high-frequency gastric electrical stimulation, Diabetes Care 27, 1071– 1076 (2004). 15111523
  32. [39]Bellinger AM, Jafari M, Grant TM, Zhang S, Slater HC, Wenger EA, Mo S, Lee Y-AL, Mazdiyasni H, Kogan L, Barman R, Cleveland C, Booth L, Bensel T, Minahan D, Hurowitz HM, Tai T, Daily J, Nikolic B, Wood L, Eckhoff PA, Langer R, Traverso G, Oral, ultra-long-lasting drug delivery: application toward malaria elimination goals., Sci. Transl. Med 8, 365ra157 (2016). PMC5264553 27856796
  33. [40]Liu J, Pang Y, Zhang S, Cleveland C, Yin X, Booth L, Lin J, Lucy Lee Y-A, Mazdiyasni H, Saxton S, Kirtane AR, von Erlach T, Rogner J, Langer R, Traverso G, Triggerable tough hydrogels for gastric resident dosage forms, Nat. Commun. 8, 124 (2017). 28743858 PMC5527117
  34. [41]Doenhoff MJ, Cioli D, Utzinger J, Praziquantel: mechanisms of action, resistance and new derivatives for schistosomiasis, Curr. Opin. Infect. Dis. 21, 659– 667 (2008). 18978535
  35. [42]Vale N, Gouveia MJ, Rinaldi G, Brindley PJ, Gärtner F, Correia da Costa JM, Praziquantel for schistosomiasis: single-drug metabolism revisited, mode of action, and resistance., Antimicrob. Agents Chemother. 61, e02582–16 (2017). PMC5404606 28264841
  36. [43]Tokura Y, Harvey S, Chen C, Wu Y, Ng DYW, Weil T, Fabrication of defined polydopamine nanostructures by DNA origami‐templated polymerization, Angew. Chemie Int. Ed. 57, 1587– 1591 (2018). PMC5817404 29211331
  37. [44]Bisaglia M, Mammi S, Bubacco L, Kinetic and structural analysis of the early oxidation products of dopamine: analysis of the interactions with α-synuclein, J. Biol. Chem. 282, 15597–15605 (2007). 17395592
  38. [45]Traverso G, Langer R, Perspective: special delivery for the gut, Nature 519, S19– S19 (2015). 25806494
  39. [46]Li J, Baird MA, Davis MA, Tai W, Zweifel LS, Waldorf KMA, Gale M Jr, Rajagopal L, Pierce RH, Gao X, Dramatic enhancement of the detection limits of bioassays via ultrafast deposition of polydopamine, Nat. Biomed. Eng. 1, 0082 (2017). PMC5654575 29082104
  40. [47]Connock M, Pover W, Catalase particles in the epithelial cells of the guinea-pig small intestine, Histochem. J. 2, 371– 380 (1970). 5525787
  41. [48]Winterwerber P, Harvey S, Ng DYW, Weil T, Photocontrolled dopamine polymerization on DNA origami with nanometer resolution, Angew. Chemie Int. Ed. 59, 6144– 6149 (2020). PMC7186833 31750608
  42. [49]Ball V, Del Frari D, Toniazzo V, Ruch D, Kinetics of polydopamine film deposition as a function of pH and dopamine concentration: Insights in the polydopamine deposition mechanism, J. Colloid Interface Sci. 386, 366– 372 (2012). 22874639
  43. [50]Holmes JC, Fowler NO, Direct cardiac effects of dopamine, Circ. Res. 10, 68– 72 (1962). 13908517
  44. [51]Barker N, Adult intestinal stem cells: critical drivers of epithelial homeostasis and regeneration, Nat. Rev. Mol. Cell Biol. 15, 19– 33 (2014). 24326621
  45. [52]Hong S, Wang Y, Park SY, Lee H, Progressive fuzzy cation-assembly of biological catecholamines, Sci. Adv. 4, eaat7457 (2018). PMC6128673 30202784
  46. [53]Ponzio F, Le Houerou V, Zafeiratos S, Gauthier C, Garnier T, Jierry L, Ball V, Robust alginate-catechol@polydopamine free-standing membranes obtained from the water/air interface, Langmuir 33, 2420– 2426 (2017). 28198631
  47. [54]Thelen K, Dressman JB, Cytochrome P450-mediated metabolism in the human gut wall, J. Pharm. Pharmacol. 61, 541– 558 (2009). 19405992
  48. [56]Kirkman HN, Gaetani GF, Mammalian catalase: a venerable enzyme with new mysteries, Trends Biochem. Sci. 32, 44– 50 (2007). 17158050
  49. [57]Heber D, Greenway FL, Kaplan LM, Livingston E, Salvador J, Still C, Endocrine and nutritional management of the post-bariatric surgery patient: An endocrine society clinical practice guideline, J. Clin. Endocrinol. Metab. 95, 4823– 4843 (2010). 21051578
  50. [58]Kirtane AR, Hua T, Hayward A, Bajpayee A, Wahane A, Lopes A, Bensel T, Ma L, Stanczyk FZ, Brooks S, Gwynne D, Wainer J, Collins J, Tamang SM, Langer R, Traverso G, A once-a-month oral contraceptive, Sci. Transl. Med. 11, eaay2602 (2019). 31801885
  51. [59]Babaee S, Pajovic S, Kirtane AR, Shi J, Caffarel-Salvador E, Hess K, Collins JE, Tamang S, Wahane AV, Hayward AM, Mazdiyasni H, Langer R, Traverso G, Temperature-responsive biometamaterials for gastrointestinal applications, Sci. Transl. Med. 11, eaau8581 (2019). PMC7797624 30996082
  52. [60]Manell E, Hedenqvist P, Svensson A, Jensen-Waern M, Xu E, Ed. Establishment of a refined oral glucose tolerance test in pigs, and assessment of insulin, glucagon and glucagon-like peptide-1 responses, PLoS One 11, e0148896 (2016). PMC4747562 26859145
  53. [61]Zhan H, Jagtiani T, Liang JF, A new targeted delivery approach by functionalizing drug nanocrystals through polydopamine coating, Eur. J. Pharm. Biopharm. 114, 221– 229 (2017). 28161549
  54. [63]Park J, Brust TF, Lee HJ, Lee SC, Watts VJ, Yeo Y, Polydopamine-based simple and versatile surface modification of polymeric nano drug carriers, ACS Nano 8, 3347– 3356 (2014). 24628245 PMC4107448
  55. [64]Margoliash E, Margoliash A, A study of the inhibition of catalase by 3-amino-1:2:4:-triazole, Biochem. J. 68, 468– 475 (1958). 13522646 PMC1200378

Cited by

Sorted by citations

Authors
Li, J., Wang, T., Kirtane, A. R., Shi, Y., Jones, A., Moussa, Z., Lopes, A., Collins, J., Tamang, S. M., Hess, K., Shakur, R., Karandikar, P., Lee, J. S., Huang, H.-W., Hayward, A., & Traverso, G.
Affiliations
  • Koch Institute for Integrative Cancer Research At MIT (US)
  • Brigham and Women's Hospital (US)
Corresponding author
Traverso, Giovanni
ORCID
Venue
Science Translational Medicine
Publisher
American Association for the Advancement of Science (AAAS)
Published
2020-08-26
Volume
12
Issue
558
ISSN
  • 1946-6234 (ISSN-L)
  • 1946-6242
DOI
10.1126/scitranslmed.abc0441
PMC
PMC8221077
OpenAlex
W3080754573
Cited by
70
References
5
Citations by year
2026: 5 · 2025: 19 · 2024: 11 · 2023: 19 · 2022: 7 · 2021: 9
FWCI
2.41
Type
journal article
Language
en
Open access
Locations
  • Science Translational Medicine (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by)
  • University of Brighton Repository (University of Brighton) (submittedVersion)
Funders
  • National Institutes of Health (EB000244)
  • Bill and Melinda Gates Foundation (OPP1179091)
Topics
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
Keywords
catalase, dopamine polymerization, small intestine, tissue adhesion, drug delivery, gastrointestinal epithelium
MeSH terms
Animals, Epithelium, Intestine, Small, Swine, Gastrointestinal Tract

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