The DOI matches its Crossref record; 21 of the 22 references it cites were found in a registry or an index.
Aspergillus fumigatus Forms Biofilms with Reduced Antifungal Drug Susceptibility on Bronchial Epithelial Cells
Open accessAbstract
Aspergillus fumigatus is a leading cause of death in immunocompromised patients and a frequent colonizer of the respiratory tracts of asthma and cystic fibrosis (CF) patients. Biofilms enable bacteria and yeasts to persist in infections and can contribute to antimicrobial resistance. We investigated the ability of A. fumigatus to form biofilms on polystyrene (PS) and human bronchial epithelial (HBE) and CF bronchial epithelial (CFBE) cells. We developed a novel in vitro coculture model of A. fumigatus biofilm formation on HBE and CFBE cells. Biofilm formation was documented by dry weight, scanning electron microscopy (SEM), and confocal scanning laser microscopy (CSLM). The in vitro antifungal activities of seven antifungal drugs were tested by comparing planktonic and sessile A. fumigatus strains. A. fumigatus formed an extracellular matrix on PS and HBE and CFBE cells as evidenced by increased dry weight, SEM, and CSLM. These biofilms exhibited decreased antifungal drug susceptibility and were adherent to the epithelial cells, with fungi remaining viable throughout 3 days. These observations might have implications for treatment of A. fumigatus colonization in chronic lung diseases and for its potential impact on airway inflammation, damage, and infection.
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“For example, a number of these biofilm formers (Table 3) have been tested for drug susceptibility in vitro, and cells in biofilms have been found to be more resistant to drugs compared with cells grown in suspension culture.”
From Candida albicans Biofilms and Human Disease (2015) · cites this paper as [176]
In the citing paper
“Aspergillus fumigatus is a common invasive colonizer of the respiratory tract in individuals with cystic fibrosis or other conditions that compromise the immune system (176).”
From Candida albicans Biofilms and Human Disease (2015) · cites this paper as [176]
In the citing paper
“These include (in addition to Candida) members from Acremonium, Aspergillus, Cladosporium, Fusarium, and Pneumocystis (9, 20, 35, 42, 79, 160, 176).”
From Candida albicans Biofilms and Human Disease (2015) · cites this paper as [176]
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Metadata
- Authors
- Seidler, M. J., Salvenmoser, S., & Müller, F.-M. C.
- Affiliations
- Heidelberg University (DE)
- Pediatric Pulmonology, Cystic Fibrosis Centre and Infectious Diseases, Department of Pediatrics III, University of Heidelberg, Im Neuenheimer Feld 430, D-69120 Heidelberg, Germany
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2008-11
- Volume
- 52
- Issue
- 11
- Pages
- 4130-4136
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC2573142
- OpenAlex
- W2098670630
- Cited by
- 229
- References
- 22
- Citations by year
- 2026: 9 · 2025: 13 · 2024: 8 · 2023: 11 · 2022: 16 · 2021: 16 · 2020: 14 · 2019: 6 · 2018: 13 · 2017: 18 · 2016: 14 · 2015: 16 · 2014: 12 · 2013: 13 · 2012: 8
- FWCI
- 9.05
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Aspergillus fumigatus, biofilm formation, antifungal susceptibility, bronchial epithelial cells, cystic fibrosis, extracellular matrix, fungal adhesion, polystyrene, antifungal resistance, chronic lung disease, airway colonization, immunocompromised patients
- MeSH terms
- Antifungal Agents, Aspergillosis, Aspergillus fumigatus, Bronchi, Cells, Cultured, Cystic Fibrosis, Epithelial Cells, Humans, Microbial Sensitivity Tests, Microscopy, Electron, Scanning, Respiratory Tract Infections, Biofilms, Microscopy, Confocal, Drug Resistance, Fungal
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