Cited by 229Antifungal resistance and susceptibilityBacterial biofilms and quorum sensingFungal and yeast genetics research

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Aspergillus fumigatus Forms Biofilms with Reduced Antifungal Drug Susceptibility on Bronchial Epithelial Cells

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Seidler, Marc J., Salvenmoser, Stefanie, Müller, Frank-Michael C.·2008·Antimicrobial Agents and Chemotherapy

DOI 10.1128/aac.00234-08PMC PMC2573142

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Abstract

Aspergillus fumigatus is a leading cause of death in immunocompromised patients and a frequent colonizer of the respiratory tracts of asthma and cystic fibrosis (CF) patients. Biofilms enable bacteria and yeasts to persist in infections and can contribute to antimicrobial resistance. We investigated the ability of A. fumigatus to form biofilms on polystyrene (PS) and human bronchial epithelial (HBE) and CF bronchial epithelial (CFBE) cells. We developed a novel in vitro coculture model of A. fumigatus biofilm formation on HBE and CFBE cells. Biofilm formation was documented by dry weight, scanning electron microscopy (SEM), and confocal scanning laser microscopy (CSLM). The in vitro antifungal activities of seven antifungal drugs were tested by comparing planktonic and sessile A. fumigatus strains. A. fumigatus formed an extracellular matrix on PS and HBE and CFBE cells as evidenced by increased dry weight, SEM, and CSLM. These biofilms exhibited decreased antifungal drug susceptibility and were adherent to the epithelial cells, with fungi remaining viable throughout 3 days. These observations might have implications for treatment of A. fumigatus colonization in chronic lung diseases and for its potential impact on airway inflammation, damage, and infection.

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Show all 22 references

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Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; M38-A 2002 vol. 22
Abstr. 46th Intersci. Conf. Antimicrob. Agents Chemother. 2006

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Authors: Seidler, M. J., Salvenmoser, S., & Müller, F.-M. C.
Affiliations: Heidelberg University (DE)
Pediatric Pulmonology, Cystic Fibrosis Centre and Infectious Diseases, Department of Pediatrics III, University of Heidelberg, Im Neuenheimer Feld 430, D-69120 Heidelberg, Germany
Venue: Antimicrobial Agents and Chemotherapy
Publisher: American Society for Microbiology
Published: 2008-11
Volume: 52
Issue: 11
Pages: 4130-4136
ISSN: 0066-4804 (ISSN-L)
1098-6596
DOI: 10.1128/aac.00234-08
PMC: PMC2573142
OpenAlex: W2098670630
Cited by: 229
References: 22
Citations by year: 2026: 9 · 2025: 13 · 2024: 8 · 2023: 11 · 2022: 16 · 2021: 16 · 2020: 14 · 2019: 6 · 2018: 13 · 2017: 18 · 2016: 14 · 2015: 16 · 2014: 12 · 2013: 13 · 2012: 8
FWCI: 9.05
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Antimicrobial Agents and Chemotherapy (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords: Aspergillus fumigatus, biofilm formation, antifungal susceptibility, bronchial epithelial cells, cystic fibrosis, extracellular matrix, fungal adhesion, polystyrene, antifungal resistance, chronic lung disease, airway colonization, immunocompromised patients
MeSH terms: Antifungal Agents, Aspergillosis, Aspergillus fumigatus, Bronchi, Cells, Cultured, Cystic Fibrosis, Epithelial Cells, Humans, Microbial Sensitivity Tests, Microscopy, Electron, Scanning, Respiratory Tract Infections, Biofilms, Microscopy, Confocal, Drug Resistance, Fungal

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Aspergillus fumigatus Forms Biofilms with Reduced Antifungal Drug Susceptibility on Bronchial Epithelial Cells

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