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Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B
Open accessAbstract
Parameters for production and purification of a bacteriocin produced by Lactobacillus acidophilus N2 are described. Production of lactacin B was pH dependent, with maximum activity detected in broth cultures maintained at pH 6. Lactacin B was purified by ion-exchange chromatography, ultrafiltration, and successive gel filtrations in the presence of 8 M urea and then 0.1% sodium dodecyl sulfate. The molecular weight of lactacin B was ca. 6,000 to 6,500, and the purified compound showed maximum absorbance at 211 nm. The activity of purified lactacin B was bactericidal to sensitive cells and restricted to members of the family Lactobacilliaceae, L. leichmannii, L. bulgaricus, L. helveticus, and L. lactis. Characteristics identified for lactacin B indicated that it was a peptide and confirmed its identity as a bacteriocin.
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Citations
Cited by
Bacteriocins of lactic acid bacteria
Klaenhammer, T. R. (1988) · Biochimie · doi:10.1016/0300-9084(88)90206-4 · Cited by 1,539
Self-citationThe Genera Lactobacillus and Carnobacterium
(2011) · SpringerReference · doi:10.1007/springerreference_3731 · Cited by 382
Lewus, C. B., Kaiser, A., & Montville, T. J. (1991) · Applied and Environmental Microbiology · doi:10.1128/aem.57.6.1683-1688.1991 · Cited by 377
Piard, J. C., & Desmazeaud, M. (1992) · Le Lait · doi:10.1051/lait:199229 · Cited by 266
Bacteriocin production: A relatively unharnessed probiotic trait?
Hegarty, J. W., Guinane, C. M., Ross, R. P., Hill, C., & Cotter, P. D. (2016) · F1000Research · doi:10.12688/f1000research.9615.1 · Cited by 141
Metadata
- Authors
- Barefoot, S. F., & Klaenhammer, T. R.
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 1984-09
- Volume
- 26
- Issue
- 3
- Pages
- 328-334
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- OpenAlex
- W2063711020
- Cited by
- 244
- References
- 0
- FWCI
- 4.03
- Type
- journal article
- Language
- en
- Open access
- green
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- Barefoot, S. F., & Klaenhammer, T. R. (1984). Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B. Antimicrobial Agents and Chemotherapy. https://doi.org/10.1128/aac.26.3.328
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