Cited by 165Antibiotic Resistance in BacteriaDrug Transport and Resistance MechanismsBacterial Genetics and Biotechnology

AcrAB Multidrug Efflux Pump Is Associated with Reduced Levels of Susceptibility to Tigecycline (GAR-936) in Proteus mirabilis

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Visalli, Melissa A., Murphy, Ellen, Projan, Steven J., Bradford, Patricia A.·2003·Antimicrobial Agents and Chemotherapy

Abstract

Tigecycline has good broad-spectrum activity against many gram-positive and gram-negative pathogens with the notable exception of the Proteeae . A study was performed to identify the mechanism responsible for the reduced susceptibility to tigecycline in Proteus mirabilis . Two independent transposon insertion mutants of P. mirabilis that had 16-fold-increased susceptibility to tigecycline were mapped to the acrB gene homolog of the Escherichia coli AcrRAB efflux system. Wild-type levels of decreased susceptibility to tigecycline were restored to the insertion mutants by complementation with a clone containing a PCR-derived fragment from the parental wild-type acrRAB efflux gene cluster. The AcrAB transport system appears to be associated with the intrinsic reduced susceptibility to tigecycline in P. mirabilis .

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Crossref · 10.1128/aac.47.2.665-669.2003

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  • MDR efflux pumps AcrAB-TolC in Enterobacteriaceae and MexAB-OprM in P. aeruginosa, both RND-family pumps, extrude tetracyclines including tigecycline as part of their contribution to multidrug resistance.

    “In addition to the tetracycline-specific transport systems, several MDR efflux pumps like AcrAB-TolC in Enterobacteriaceae and MexAB-OprM in P. aeruginosa (both belonging to the RND family) are able to extrude tetracyclines (including tigecycline) as part of their contribution to multidrug resistance (48, 49).”

    From Mechanisms of Antibiotic Resistance (2016) · cites this paper as [48]

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Authors
Visalli, M. A., Murphy, E., Projan, S. J., & Bradford, P. A.
Affiliations
Wyeth (US)
Corresponding author
Visalli, Melissa A.
Venue
Antimicrobial Agents and Chemotherapy
Published
2003-02
Volume
47
Issue
2
Pages
665-669
ISSN
  • 0066-4804 (ISSN-L)
  • 1098-6596
OpenAlex
W1976898407
Cited by
165
References
25
Citations by year
2026: 2 · 2025: 2 · 2024: 5 · 2023: 6 · 2022: 4 · 2021: 5 · 2020: 5 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 9 · 2015: 7 · 2014: 2 · 2013: 6 · 2012: 4
FWCI
7.83
Type
journal article
Language
en
Open access
Locations
  • Antimicrobial Agents and Chemotherapy (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
tigecycline, Proteus mirabilis, AcrAB efflux pump, multidrug efflux, transposon mutants, complementation, tetracycline resistance
MeSH terms
Tigecycline, Carrier Proteins, DNA Transposable Elements, Membrane Proteins, Minocycline, Proteus mirabilis, Drug Resistance, Bacterial, ATP-Binding Cassette, Sub-Family C Proteins, Escherichia coli Proteins

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