The DOI matches its Crossref record; 43 of the 44 references it cites were found in a registry or an index.
Lactobacillus plantarum WCFS1 Electron Transport Chains
Open accessAbstract
Lactobacillus plantarum WCFS1 requires both heme and menaquinone to induce respiration-like behavior under aerobic conditions. The addition of these compounds enhanced both biomass production, without progressive acidification, and the oxygen consumption rate. When both heme and menaquinone were present, L. plantarum WCFS1 was also able to reduce nitrate. The ability to reduce nitrate was severely inhibited by the glucose levels that are typically found in L. plantarum growth media (1 to 2% [vol/vol] glucose). In contrast, comparable mannitol levels did not inhibit the reduction of nitrate. L. plantarum reduced nitrate with concomitant formation of nitrite and ammonia. Genes that encode a bd-type cytochrome (cydABCD) and a nitrate reductase (narGHJI) were identified in the genome of L. plantarum. The narGHJI operon is part of a cluster of genes that includes the molybdopterin cofactor biosynthesis genes and narK. Besides a menaquinone source, isogenic mutants revealed that cydA and ndh1 are required for the aerobic-respiration-like response and narG for nitrate reduction. The ndh1 mutant was still able to reduce nitrate. The existence of a nonredundant branched electron transport chain in L. plantarum WCFS1 that is capable of using oxygen or nitrate as a terminal electron acceptor is proposed.
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Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular Cloning. A Laboratory Manual
Wood, E. (1983) · Biochemical Education · doi:10.1016/0307-4412(83)90068-7 · Cited by 29,534
Cell biology and molecular basis of denitrification
Zumft, W. G. (1997) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.61.4.533-616.1997 · Cited by 3,560
Complete genome sequence of Lactobacillus plantarum WCFS1
Kleerebezem, M., Boekhorst, J., van Kranenburg, R., Molenaar, D., Kuipers, O. P., Leer, R., Tarchini, R., Peters, S. A., Sandbrink, H. M., Fiers, M. W. E. J., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0337704100 · Cited by 1,472
Unden, G., & Bongaerts, J. (1997) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(97)00034-0 · Cited by 744
The normal Lactobacillus flora of healthy human rectal and oral mucosa
Ahrné, Nobaek, Jeppsson, Adlerberth, Wold, A. E., & Molin (1998) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.1998.00480.x · Cited by 439
Nitrate, nitrite and N-nitroso compounds
Gangolli, S. D., van den Brandt, P. A., Feron, V. J., Janzowsky, C., Koeman, J. H., Speijers, G. J. A., Spiegelhalder, B., Walker, R., & Wishnok, J. S. (1994) · European Journal of Pharmacology: Environmental Toxicology and Pharmacology · doi:10.1016/0926-6917(94)90022-1 · Cited by 380
Dietary nitrate in man: friend or foe?
McKnight, G. M., Duncan, C. W., Leifert, C., & Golden, M. H. (1999) · British Journal of Nutrition · doi:10.1017/s000711459900063x · Cited by 300
Duwat, P., Sourice, S., Cesselin, B., Lamberet, G., Vido, K., Gaudu, P., Loir, Y. L., Violet, F., Loubière, P., & Gruss, A. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.15.4509-4516.2001 · Cited by 245
Cytochrome bd terminal oxidase1All amino acid numbering refers to the E. coli enzyme.1
Jünemann, S. (1997) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(97)00046-7 · Cited by 244
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1 of the 44 references couldn’t be matched to a known paper.
Research works printed but not linked: 3
- Arneth, W., and B. Herold. 1988. Nitrat/Nitrit-Bestimmung in Wurstwaren nach enzymatischer Reduction. Fleischwirtschaft68:761-764.
- Beutler, H.-O., B. Wurst, and S. Fisher. 1986. Eine neue Methode zur enzymatischen Bestimmung van Nitrat in Lebensmitteln. Dtsch. Lebensmittel-Rundschau82:283-289.
- Kretzschmar, R., and T. Kretschmar. 1988. Enzymatische Nitrat-Bestimmung in kommunalen Abwasser. Vom Abwasser70:119-128.
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Menaquinone biosynthesis in Lactococcus lactis: Recent advances and future prospects
Lou, Y., Shuang, J., Kong, J., & Guo, T. (2026) · Engineering Microbiology · doi:10.1016/j.engmic.2026.100304
Enhanced D-Lactic Acid Production from Dairy Residues Using Engineered Lactiplantibacillus plantarum
Enzien, G., Klein, A. G., Urbanz, A., Oh, J., Donohue, T. J., & Noguera, D. R. (2026) · Fermentation · doi:10.3390/fermentation12110509
Metadata
- Authors
- Brooijmans, R. J. W., de Vos, W. M., & Hugenholtz, J.
- Affiliations
- TiFN (NL)
- Department of General Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands
- ORCID
- Hugenholtz, J.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2009-06
- Volume
- 75
- Issue
- 11
- Pages
- 3580-3585
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2687314
- OpenAlex
- W2036330187
- Cited by
- 103
- References
- 44
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 6 · 2023: 9 · 2022: 8 · 2021: 7 · 2020: 3 · 2019: 9 · 2018: 2 · 2017: 11 · 2016: 5 · 2015: 4 · 2014: 5 · 2013: 6 · 2012: 7
- FWCI
- 3.68
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Keywords
- Lactobacillus plantarum WCFS1, electron transport chain, aerobic respiration, heme, vitamin K2, nitrate reduction, nitrate reductase, narG, terminal electron acceptors, glucose inhibition, nitrite production, ammonia formation
- MeSH terms
- Aerobiosis, Ammonia, Bacterial Proteins, Electron Transport, Genes, Bacterial, Glucose, Heme, Mannitol, Nitrates, Nitrites, Oxidation-Reduction, Oxygen, Gene Order, Vitamin K 2, Lactiplantibacillus plantarum, Gene Knockout Techniques
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