Cited by 111Photosynthetic Processes and MechanismsAlgal biology and biofuel productionBacterial Genetics and Biotechnology

Carbohydrate Metabolism in Mutants of the Cyanobacterium Synechococcus elongatus PCC 7942 Defective in Glycogen Synthesis

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Suzuki, Eiji, Ohkawa, Hajime, Moriya, Katsuya, Matsubara, Tatsuya, Nagaike, Yukari, Iwasaki, Ikuko, Fujiwara, Shoko, Tsuzuki, Mikio +1 more·2010·Applied and Environmental Microbiology

Abstract

ADP-glucose pyrophosphorylase (AGPase) and glycogen synthase (GS) catalyze the first two reactions of glycogen synthesis in cyanobacteria. Mutants defective in each of these enzymes in Synechococcus elongatus PCC 7942 were constructed and characterized. Activities of the corresponding enzymes in the selected mutants were virtually undetectable, and their ability to synthesize glycogen was entirely abolished. The maximal activities of photosynthetic O 2 evolution and the rates of respiration in the dark were significantly decreased in the mutants compared to those in wild-type cells. Addition of 0.2 M NaCl or 3 mM H 2 O 2 to liquid cultures markedly inhibited the growth of the AGPase and GS mutants, while the same treatment had only marginal effects on the wild type. These results suggest a significant role for storage polysaccharides in tolerance to salt or oxidative stress.

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Crossref · 10.1128/aem.00397-08

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Authors
Suzuki, E., Ohkawa, H., Moriya, K., Matsubara, T., Nagaike, Y., Iwasaki, I., Fujiwara, S., Tsuzuki, M., & Nakamura, Y.
Affiliations
  • Department of Biological Production
  • Tokyo University of Pharmacy and Life Sciences (JP)
  • Akita Prefectural University (JP)
Corresponding author
Suzuki, Eiji
Venue
Applied and Environmental Microbiology
Published
2010-05-15
Volume
76
Issue
10
Pages
3153-3159
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2100920419
Cited by
111
References
42
Citations by year
2026: 3 · 2025: 1 · 2024: 3 · 2023: 5 · 2022: 7 · 2021: 5 · 2020: 5 · 2019: 13 · 2018: 13 · 2017: 11 · 2016: 7 · 2015: 11 · 2014: 7 · 2013: 10 · 2012: 7
FWCI
2.59
Type
journal article
Language
en
Open access
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
University of Tokyo
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
glycogen synthesis, ADP-glucose pyrophosphorylase, glycogen synthase, Synechococcus elongatus PCC 7942, cyanobacteria, carbohydrate metabolism, mutant analysis, photosynthetic oxygen evolution, respiration, salt stress, oxidative stress, storage polysaccharides, stress tolerance
MeSH terms
Chlorophyll A, Carbohydrates, Chlorophyll, Glycogen, Glycogen Synthase, Hydrogen Peroxide, Mutation, Oxygen, Photosynthesis, Phycocyanin, Sodium Chloride, Oxidative Stress, Synechococcus, Carbohydrate Metabolism, Glucose-1-Phosphate Adenylyltransferase

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