Carbohydrate Metabolism in Mutants of the Cyanobacterium Synechococcus elongatus PCC 7942 Defective in Glycogen Synthesis
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ADP-glucose pyrophosphorylase (AGPase) and glycogen synthase (GS) catalyze the first two reactions of glycogen synthesis in cyanobacteria. Mutants defective in each of these enzymes in Synechococcus elongatus PCC 7942 were constructed and characterized. Activities of the corresponding enzymes in the selected mutants were virtually undetectable, and their ability to synthesize glycogen was entirely abolished. The maximal activities of photosynthetic O 2 evolution and the rates of respiration in the dark were significantly decreased in the mutants compared to those in wild-type cells. Addition of 0.2 M NaCl or 3 mM H 2 O 2 to liquid cultures markedly inhibited the growth of the AGPase and GS mutants, while the same treatment had only marginal effects on the wild type. These results suggest a significant role for storage polysaccharides in tolerance to salt or oxidative stress.
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In S. elongatus PCC 7942, deletions in the glgC and glgA genes strongly repressed glycogen accumulation.
“In S. elongatus PCC 7942, deletions in the glgC and glgA genes strongly repressed glycogen accumulation (Suzuki et al. 2010).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Suzuki et al. 2010
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- Authors
- Suzuki, E., Ohkawa, H., Moriya, K., Matsubara, T., Nagaike, Y., Iwasaki, I., Fujiwara, S., Tsuzuki, M., & Nakamura, Y.
- Affiliations
- Department of Biological Production
- Tokyo University of Pharmacy and Life Sciences (JP)
- Akita Prefectural University (JP)
- Corresponding author
- Suzuki, Eiji
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-05-15
- Volume
- 76
- Issue
- 10
- Pages
- 3153-3159
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2869141
- OpenAlex
- W2100920419
- Cited by
- 111
- References
- 42
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 3 · 2023: 5 · 2022: 7 · 2021: 5 · 2020: 5 · 2019: 13 · 2018: 13 · 2017: 11 · 2016: 7 · 2015: 11 · 2014: 7 · 2013: 10 · 2012: 7
- FWCI
- 2.59
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Tokyo
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- glycogen synthesis, ADP-glucose pyrophosphorylase, glycogen synthase, Synechococcus elongatus PCC 7942, cyanobacteria, carbohydrate metabolism, mutant analysis, photosynthetic oxygen evolution, respiration, salt stress, oxidative stress, storage polysaccharides, stress tolerance
- MeSH terms
- Chlorophyll A, Carbohydrates, Chlorophyll, Glycogen, Glycogen Synthase, Hydrogen Peroxide, Mutation, Oxygen, Photosynthesis, Phycocyanin, Sodium Chloride, Oxidative Stress, Synechococcus, Carbohydrate Metabolism, Glucose-1-Phosphate Adenylyltransferase
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