The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Pepper Mild Mottle Virus as an Indicator of Fecal Pollution
Open accessAbstract
Accurate indicators of fecal pollution are needed in order to minimize public health risks associated with wastewater contamination in recreational waters. However, the bacterial indicators currently used for monitoring water quality do not correlate with the presence of pathogens. Here we demonstrate that the plant pathogen Pepper mild mottle virus (PMMoV) is widespread and abundant in wastewater from the United States, suggesting the utility of this virus as an indicator of human fecal pollution. Quantitative PCR was used to determine the abundance of PMMoV in raw sewage, treated wastewater, seawater exposed to wastewater, and fecal samples and/or intestinal homogenates from a wide variety of animals. PMMoV was present in all wastewater samples at concentrations greater than 1 million copies per milliliter of raw sewage. Despite the ubiquity of PMMoV in human feces, this virus was not detected in the majority of animal fecal samples tested, with the exception of chicken and seagull samples. PMMoV was detected in four out of six seawater samples collected near point sources of secondary treated wastewater off southeastern Florida, where it co-occurred with several other pathogens and indicators of fecal pollution. Since PMMoV was not found in nonpolluted seawater samples and could be detected in surface seawater for approximately 1 week after its initial introduction, the presence of PMMoV in the marine environment reflects a recent contamination event. Together, these data demonstrate that PMMoV is a promising new indicator of fecal pollution in coastal environments.
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Cites
RNA Viral Community in Human Feces: Prevalence of Plant Pathogenic Viruses
Zhang, T., Breitbart, M., Lee, W. H., Run, J.-Q., Wei, C. L., Soh, S. W. L., Hibberd, M. L., Liu, E. T., Rohwer, F., & Ruan, Y. (2005) · PLoS Biology · doi:10.1371/journal.pbio.0040003 · Cited by 805
Self-citationFong, T.-T., & Lipp, E. K. (2005) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.69.2.357-371.2005 · Cited by 795
Bernhard, A. E., & Field, K. G. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.10.4571-4574.2000 · Cited by 692
Kildare, B. J., Leutenegger, C. M., McSwain, B. S., Bambic, D. G., Rajal, V. B., & Wuertz, S. (2007) · Water Research · doi:10.1016/j.watres.2007.06.037 · Cited by 451
SAVICHTCHEVA, O., & OKABE, S. (2006) · Water Research · doi:10.1016/j.watres.2006.04.040 · Cited by 381
Jothikumar, N., Lowther, J. A., Henshilwood, K., Lees, D. N., Hill, V. R., & Vinjé, J. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.4.1870-1875.2005 · Cited by 367
Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices
Bofill-Mas, S., Albinana-Gimenez, N., Clemente-Casares, P., Hundesa, A., Rodriguez-Manzano, J., Allard, A., Calvo, M., & Girones, R. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.00965-06 · Cited by 327
Pathogenic Human Viruses in Coastal Waters
Griffin, D. W., Donaldson, K. A., Paul, J. H., & Rose, J. B. (2003) · Clinical Microbiology Reviews · doi:10.1128/cmr.16.1.129-143.2003 · Cited by 294
Lipp, E. K., Farrah, S. A., & Rose, J. B. (2001) · Marine Pollution Bulletin · doi:10.1016/s0025-326x(00)00152-1 · Cited by 260
New tools for the study and direct surveillance of viral pathogens in water
Bosch, A., Guix, S., Sano, D., & Pintó, R. M. (2008) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2008.04.006 · Cited by 237
Cited by
Haramoto, E., Malla, B., Thakali, O., & Kitajima, M. (2020) · Science of The Total Environment · doi:10.1016/j.scitotenv.2020.140405 · Cited by 593
Haramoto, E., Kitajima, M., Hata, A., Torrey, J. R., Masago, Y., Sano, D., & Katayama, H. (2018) · Water Research · doi:10.1016/j.watres.2018.02.004 · Cited by 432
D'Aoust, P. M., Mercier, E., Montpetit, D., Jia, J.-J., Alexandrov, I., Neault, N., Baig, A. T., Mayne, J., Zhang, X., Alain, T., et al. (2021) · Water Research · doi:10.1016/j.watres.2020.116560 · Cited by 382
Rački, N., Dreo, T., Gutierrez-Aguirre, I., Blejec, A., & Ravnikar, M. (2014) · Plant Methods · doi:10.1186/s13007-014-0042-6 · Cited by 257
Uyaguari-Diaz, M. I., Chan, M., Chaban, B. L., Croxen, M. A., Finke, J. F., Hill, J. E., Peabody, M. A., Van Rossum, T., Suttle, C. A., Brinkman, F. S. L., et al. (2016) · Microbiome · doi:10.1186/s40168-016-0166-1 · Cited by 104
Metadata
- Authors
- Rosario, K., Symonds, E. M., Sinigalliano, C., Stewart, J., & Breitbart, M.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2009-11-15
- Volume
- 75
- Issue
- 22
- Pages
- 7261-7267
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2151575975
- Cited by
- 339
- References
- 22
- FWCI
- 5.43
- Type
- journal article
- Language
- en
- Open access
- green
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