The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Fluorescence-Based Reporter for Gauging Cyclic Di-GMP Levels in Pseudomonas aeruginosa
Open accessAbstract
The increased tolerance toward the host immune system and antibiotics displayed by biofilm-forming Pseudomonas aeruginosa and other bacteria in chronic infections such as cystic fibrosis bronchopneumonia is of major concern. Targeting of biofilm formation is believed to be a key aspect in the development of novel antipathogenic drugs that can augment the effect of classic antibiotics by decreasing antimicrobial tolerance. The second messenger cyclic di-GMP is a positive regulator of biofilm formation, and cyclic di-GMP signaling is now regarded as a potential target for the development of antipathogenic compounds. Here we describe the development of fluorescent monitors that can gauge the cellular level of cyclic di-GMP in P. aeruginosa . We have created cyclic di-GMP level reporters by transcriptionally fusing the cyclic di-GMP-responsive cdrA promoter to genes encoding green fluorescent protein. We show that the reporter constructs give a fluorescent readout of the intracellular level of cyclic di-GMP in P. aeruginosa strains with different levels of cyclic di-GMP. Furthermore, we show that the reporters are able to detect increased turnover of cyclic di-GMP mediated by treatment of P. aeruginosa with the phosphodiesterase inducer nitric oxide. Considering that biofilm formation is a necessity for the subsequent development of a chronic infection and therefore a pathogenicity trait, the reporters display a significant potential for use in the identification of novel antipathogenic compounds targeting cyclic di-GMP signaling, as well as for use in research aiming at understanding the biofilm biology of P. aeruginosa .
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1 retracted
Ryan, R. P., Fouhy, Y., Lucey, J. F., Crossman, L. C., Spiro, S., He, Y.-W., Zhang, L.-H., Heeb, S., Cámara, M., Williams, P., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0600345103 · Cited by 507
RetractedBacterial Biofilms: A Common Cause of Persistent Infections
Costerton, J. W., Stewart, P. S., & Greenberg, E. P. (1999) · Science · doi:10.1126/science.284.5418.1318 · Cited by 12,167
Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
Stover, C. K., Pham, X. Q., Erwin, A. L., Mizoguchi, S. D., Warrener, P., Hickey, M. J., Brinkman, F. S. L., Hufnagle, W. O., Kowalik, D. J., Lagrou, M., et al. (2000) · Nature · doi:10.1038/35023079 · Cited by 4,573
Hoang, T. T., Karkhoff-Schweizer, R. R., Kutchma, A. J., & Schweizer, H. P. (1998) · Gene · doi:10.1016/s0378-1119(98)00130-9 · Cited by 1,922
Herrero, M., de Lorenzo, V, & Timmis, K. N. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.11.6557-6567.1990 · Cited by 1,586
Attenuation of Pseudomonas aeruginosa virulence by quorum sensing inhibitors
Hentzer, M. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg366 · Cited by 1,422
Self-citationHentzer, M., Riedel, K., Rasmussen, T. B., Heydorn, A., Andersen, J. B., Parsek, M. R., Rice, S. A., Eberl, L., Molin, S., Høiby, N., et al. (2002) · Microbiology · doi:10.1099/00221287-148-1-87 · Cited by 1,050
Self-citationAndersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.6.2240-2246.1998 · Cited by 1,033
Self-citationChoi, K.-H., Kumar, A., & Schweizer, H. P. (2006) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2005.06.001 · Cited by 988
DNA replication and the division cycle in Escherichia coli
Clark, D. J., & Maaløe, O. (1967) · Journal of Molecular Biology · doi:10.1016/s0022-2836(67)80070-6 · Cited by 962
Cited by
Cyclic di-GMP: second messenger extraordinaire
Jenal, U., Reinders, A., & Lori, C. (2017) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.190 · Cited by 1,039
Rumbaugh, K. P., & Sauer, K. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-020-0385-0 · Cited by 907
Biofilms deform soft surfaces and disrupt epithelia
Cont, A., Rossy, T., Al-Mayyah, Z., & Persat, A. (2020) · eLife · doi:10.7554/elife.56533 · Cited by 70
Biofilm formation-defective mutants in Pseudomonas putida
López-Sánchez, A., Leal-Morales, A., Jiménez-Díaz, L., Platero, A. I., Bardallo-Pérez, J., Díaz-Romero, A., Acemel, R. D., Illán, J. M., Jiménez-López, J., & Govantes, F. (2016) · FEMS Microbiology Letters · doi:10.1093/femsle/fnw127 · Cited by 31
Corver, J., Cordo’, V., van Leeuwen, H. C., Klychnikov, O. I., & Hensbergen, P. J. (2017) · Molecular Microbiology · doi:10.1111/mmi.13736 · Cited by 20
Metadata
- Authors
- Rybtke, M. T., Borlee, B. R., Murakami, K., Irie, Y., Hentzer, M., Nielsen, T. E., Givskov, M., Parsek, M. R., & Tolker-Nielsen, T.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2012-08
- Volume
- 78
- Issue
- 15
- Pages
- 5060-5069
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2096163314
- Cited by
- 287
- References
- 46
- FWCI
- 3.95
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Rybtke, M. T., Borlee, B. R., Murakami, K., Irie, Y., Hentzer, M., Nielsen, T. E., Givskov, M., Parsek, M. R., & Tolker-Nielsen, T. (2012). Fluorescence-Based Reporter for Gauging Cyclic Di-GMP Levels in Pseudomonas aeruginosa. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.00414-12
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