The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Microbial Community Development in a Dynamic Gut Model Is Reproducible, Colon Region Specific, and Selective for Bacteroidetes and Clostridium Cluster IX
Open accessAbstract
Dynamic, multicompartment in vitro gastrointestinal simulators are often used to monitor gut microbial dynamics and activity. These reactors need to harbor a microbial community that is stable upon inoculation, colon region specific, and relevant to in vivo conditions. Together with the reproducibility of the colonization process, these criteria are often overlooked when the modulatory properties from different treatments are compared. We therefore investigated the microbial colonization process in two identical simulators of the human intestinal microbial ecosystem (SHIME), simultaneously inoculated with the same human fecal microbiota with a high-resolution phylogenetic microarray: the human intestinal tract chip (HITChip). Following inoculation of the in vitro colon compartments, microbial community composition reached steady state after 2 weeks, whereas 3 weeks were required to reach functional stability. This dynamic colonization process was reproducible in both SHIME units and resulted in highly diverse microbial communities which were colon region specific, with the proximal regions harboring saccharolytic microbes (e.g.,Bacteroides spp. and Eubacterium spp.) and the distal regions harboring mucin-degrading microbes (e.g.,Akkermansia spp.). Importantly, the shift from an in vivo to an in vitro environment resulted in an increased Bacteroidetes/Firmicutes ratio, whereas Clostridium cluster IX (propionate producers) was enriched compared to clusters IV and XIVa (butyrate producers). This was supported by proportionally higher in vitro propionate concentrations. In conclusion, high-resolution analysis of in vitro-cultured gut microbiota offers new insight on the microbial colonization process and indicates the importance of digestive parameters that may be crucial in the development of new in vitro models.
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Cites
An obesity-associated gut microbiome with increased capacity for energy harvest
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The Effect of Diet on the Human Gut Microbiome: A Metagenomic Analysis in Humanized Gnotobiotic Mice
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“Such new strategies are often evaluated during human trials or in vivo studies of animals associated with conventional or human microbiota (50).”
BackgroundAkkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium
Derrien, M., Vaughan, E. E., Plugge, C. M., & de Vos, W. M. (2004) · International Journal of Systematic and Evolutionary Microbiology · doi:10.1099/ijs.0.02873-0 · Cited by 2,094
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Wu, H., Esteve, E., Tremaroli, V., Khan, M. T., Caesar, R., Mannerås-Holm, L., Ståhlman, M., Olsson, L. M., Serino, M., Planas-Fèlix, M., et al. (2017) · Nature Medicine · doi:10.1038/nm.4345 · Cited by 1,632
Algal Proteins: Extraction, Application, and Challenges Concerning Production
Bleakley, S., & Hayes, M. (2017) · Foods · doi:10.3390/foods6050033 · Cited by 1,092
Akkermansia muciniphila and its role in regulating host functions
Derrien, M., Belzer, C., & de Vos, W. M. (2017) · Microbial Pathogenesis · doi:10.1016/j.micpath.2016.02.005 · Cited by 1,044
Self-citationA complex human gut microbiome cultured in an anaerobic intestine-on-a-chip
Jalili-Firoozinezhad, S., Gazzaniga, F. S., Calamari, E. L., Camacho, D. M., Fadel, C. W., Bein, A., Swenor, B., Nestor, B., Cronce, M. J., Tovaglieri, A., et al. (2019) · Nature Biomedical Engineering · doi:10.1038/s41551-019-0397-0 · Cited by 855
Espey, M. G. (2013) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2012.10.554 · Cited by 396
Can dynamic in vitro digestion systems mimic the physiological reality?
Dupont, D., Alric, M., Blanquet-Diot, S., Bornhorst, G., Cueva, C., Deglaire, A., Denis, S., Ferrua, M., Havenaar, R., Lelieveld, J., et al. (2019) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2017.1421900 · Cited by 345
Self-citationImpact of polyphenols from black tea and red wine/grape juice on a gut model microbiome
Kemperman, R. A., Gross, G., Mondot, S., Possemiers, S., Marzorati, M., Van de Wiele, T., Doré, J., & Vaughan, E. E. (2013) · Food Research International · doi:10.1016/j.foodres.2013.01.034 · Cited by 223
Self-citationSalazar, N., Gueimonde, M., de los Reyes-Gavilán, C. G., & Ruas-Madiedo, P. (2016) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2013.770728 · Cited by 188
Joly, C., Gay-Quéheillard, J., Léké, A., Chardon, K., Delanaud, S., Bach, V., & Khorsi-Cauet, H. (2013) · Environmental Science and Pollution Research · doi:10.1007/s11356-012-1283-4 · Cited by 135
Van den Abbeele, P., Deyaert, S., Albers, R., Baudot, A., & Mercenier, A. (2023) · Nutrients · doi:10.3390/nu15092090 · Cited by 46
Van den Abbeele, P., Duysburgh, C., Cleenwerck, I., Albers, R., Marzorati, M., & Mercenier, A. (2021) · Microorganisms · doi:10.3390/microorganisms9102142 · Cited by 31
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Metadata
- Authors
- Van den Abbeele, P., Grootaert, C., Marzorati, M., Possemiers, S., Verstraete, W., Gérard, P., Rabot, S., Bruneau, A., El Aidy, S., Derrien, M., Zoetendal, E., Kleerebezem, M., Smidt, H., & Van de Wiele, T.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-08
- Volume
- 76
- Issue
- 15
- Pages
- 5237-5246
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2111317067
- Cited by
- 345
- References
- 5
- FWCI
- 5.43
- Type
- journal article
- Language
- en
- Open access
- green
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