Inactivation of Adhesion and Invasion of Food-Borne Listeria monocytogenes by Bacteriocin-Producing Bifidobacterium Strains of Human Origin

Moroni, Olivier; Kheadr, Ehab; Boutin, Yvan; Lacroix, Christophe; Fliss, Ismaïl

doi:10.1128/aem.00928-06

Cited by 116Probiotics and Fermented FoodsListeria monocytogenes in Food SafetyInfant Nutrition and Health

Inactivation of Adhesion and Invasion of Food-Borne Listeria monocytogenes by Bacteriocin-Producing Bifidobacterium Strains of Human Origin

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Moroni, Olivier, Kheadr, Ehab, Boutin, Yvan, Lacroix, Christophe, Fliss, Ismaïl·2006·Applied and Environmental Microbiology

DOI 10.1128/aem.00928-06PMC PMC1636179

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Abstract

Three bacteriocin-producing bifidobacterial isolates from newborns were identified as Bifidobacterium thermacidophilum (two strains) and B. thermophilum (one strain). This study was undertaken to evaluate the ability of these strains to compete with food-borne Listeria monocytogenes for adhesion and invasion sites on Caco-2 and HT-29 cells. The bifidobacteria adhered at levels ranging from 4% to 10% of the CFU added, but none of the bifidobacteria were able to invade cells. The abilities of Listeria to adhere to and to invade cells varied widely depending on the strain tested. Three groups of Listeria were identified based on invasiveness: weakly invasive, moderately invasive, and highly invasive strains. One strain from each group was tested in competition with bifidobacteria. B. thermacidophilum RBL70 was the most effective in blocking invasion of Listeria , and the decreases in invasion ranged from 38% to 90%. For all three bifidobacterial strains, contact between the cell monolayer and the bifidobacteria for 1 h before exposure to Listeria increased the degree of inhibition. Finally, visualization of competition for adhesion sites on cells by fluorescent in situ hybridization suggested that the two bacteria tended to adhere in close proximity.

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Numerous in vitro studies have demonstrated that bifidobacteria can inhibit pathogens through the production of organic acids, antibacterial peptides, quorum-sensing inhibitors, or immune stimulation among other mechanisms.
“Numerous in vitro studies have demonstrated that bifidobacteria can inhibit pathogens through the production of organic acids (38), antibacterial peptides (39), quorum-sensing inhibitors (40, 41), or immune stimulation (42) among other mechanisms, providing molecular clues of their capacity to prevent certain infections.”
From Bifidobacteria and Their Health-Promoting Effects (2017) · cites this paper as [39]

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Authors: Moroni, O., Kheadr, E., Boutin, Y., Lacroix, C., & Fliss, I.
Affiliations: Université Laval (CA)
Cégep de Lévis (CA)
ETH Zurich (CH)
STELA Dairy Research Center, Nutraceuticals and Functional Foods Institute (INAF), Pavillon Paul Comtois, Université Laval, Québec, Québec, Canada G1K 7P4
ORCID: Boutin, Yvan
Lacroix, Christophe
Venue: Applied and Environmental Microbiology
Publisher: American Society for Microbiology
Published: 2006-11
Volume: 72
Issue: 11
Pages: 6894-6901
ISSN: 0099-2240 (ISSN-L)
1098-5336
DOI: 10.1128/aem.00928-06
PMC: PMC1636179
OpenAlex: W2051024501
Cited by: 116
References: 44
Citations by year: 2026: 7 · 2025: 6 · 2024: 4 · 2023: 6 · 2022: 7 · 2021: 11 · 2020: 7 · 2019: 2 · 2018: 7 · 2017: 14 · 2016: 6 · 2015: 5 · 2014: 9 · 2013: 4 · 2012: 7
FWCI: 4.83
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Applied and Environmental Microbiology (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Listeria monocytogenes in Food Safety
Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
Keywords: Listeria monocytogenes, Bifidobacterium thermophilum, competitive exclusion, bacterial adhesion, cell invasion, Caco-2 cells, HT-29 cells, foodborne pathogens, neonates, fluorescence in situ hybridization
MeSH terms: Animals, Antibiosis, Bacterial Adhesion, Bacteriocins, Bifidobacterium, Dairy Products, Eggs, Feces, Humans, Infant, Listeria, Listeria monocytogenes, Milk, Caco-2 Cells, HT29 Cells

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Inactivation of Adhesion and Invasion of Food-Borne Listeria monocytogenes by Bacteriocin-Producing Bifidobacterium Strains of Human Origin

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