Cited by 276Infant Nutrition and HealthGut microbiota and healthProbiotics and Fermented Foods

Bifidobacteria isolated from infants and cultured on human milk oligosaccharides affect intestinal epithelial function

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Chichlowski, Maciej, De Lartigue, Guillaume, German, J. Bruce, Raybould, Helen E., Mills, David A.·2012·Journal of Pediatric Gastroenterology and Nutrition

Abstract

OBJECTIVES: Human milk oligosaccharides (HMOs) are the third most abundant component of breast milk. Our laboratory has previously revealed gene clusters specifically linked to HMO metabolism in selected bifidobacteria isolated from fecal samples of infants. Our objective was to test the hypothesis that growth of selected bifidobacteria on HMO stimulates the intestinal epithelium. METHODS: Caco-2 and HT-29 cells were incubated with lactose (LAC)- or HMO-grown Bifidobacterium longum subsp infantis (B infantis) or B bifidum. Bacterial adhesion and translocation were measured by real-time quantitative polymerase chain reaction. Expression of pro- and anti-inflammatory cytokines and tight junction proteins was analyzed by real-time reverse transcriptase. Distribution of tight junction proteins was measured using immunofluorescent microscopy. RESULTS: We showed that HMO-grown B infantis had a significantly higher rate of adhesion to HT-29 cells compared with B bifidum. B infantis also induced expression of a cell membrane glycoprotein, P-selectin glycoprotein ligand-1. Both B infantis and B bifidum grown on HMO caused less occludin relocalization and higher expression of anti-inflammatory cytokine, interleukin-10 compared with LAC-grown bacteria in Caco-2 cells. B bifidum grown on HMO showed higher expression of junctional adhesion molecule and occludin in Caco-2 cells and HT-29 cells. There were no significant differences between LAC or HMO treatments in bacterial translocation. CONCLUSIONS: The study provides evidence for the specific relation between HMO-grown bifidobacteria and intestinal epithelial cells. To our knowledge, this is the first study describing HMO-induced changes in the bifidobacteria-intestinal cells interaction.

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Authors
Chichlowski, M., De Lartigue, G., German, J. B., Raybould, H. E., & Mills, D. A.
Affiliations
  • University of California, Davis (US)
  • Foods for Health Institute University of California Davis CA
Corresponding author
Mills, David A.
Venue
Journal of Pediatric Gastroenterology and Nutrition
Publisher
Wiley
Published
2012-09
Volume
55
Issue
3
Pages
321-327
ISSN
  • 0277-2116 (ISSN-L)
  • 1536-4801
OpenAlex
W2017988783
Cited by
276
References
72
Citations by year
2026: 13 · 2025: 15 · 2024: 24 · 2023: 23 · 2022: 26 · 2021: 23 · 2020: 30 · 2019: 16 · 2018: 21 · 2017: 21 · 2016: 11 · 2015: 17 · 2014: 18 · 2013: 14 · 2012: 3
FWCI
15.46
Type
journal article
Language
en
Open access
Locations
  • Journal of Pediatric Gastroenterology and Nutrition (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • California Dairy Research Foundation
  • National Institute of Environmental Health Sciences (P42 ES02710)
  • National Institutes of Health (R01HD059127, R01HD061923, R21AT006180)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Keywords
human milk oligosaccharides, Bifidobacterium, Bifidobacterium longum subsp. infantis, Bifidobacterium bifidum, intestinal epithelial cells, bacterial adhesion, bacterial translocation, tight junction proteins, occludin, PSGL-1, IL-10, anti-inflammatory cytokines, lactose, infants, gut microbiota
MeSH terms
Bacterial Adhesion, Bifidobacterium, Cell Membrane, Colon, Culture Media, Humans, Intestinal Mucosa, Membrane Glycoproteins, Milk, Human, Oligosaccharides, Interleukin-10, Caco-2 Cells, HT29 Cells, Junctional Adhesion Molecules, Occludin

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