Cited by 397Probiotics and Fermented FoodsGut microbiota and healthInfant Nutrition and Health

Bifidobacterium strains from resident infant human gastrointestinal microflora exert antimicrobial activity

Open access
Liévin, V, Peiffer, I, Hudault, S, Rochat, F, Brassart, D, Neeser, J-R, Servin, A L·2000·Gut

Abstract

BACKGROUND AND AIMS: The gastrointestinal microflora exerts a barrier effect against enteropathogens. The aim of this study was to examine if bifidobacteria, a major species of the human colonic microflora, participates in the barrier effect by developing antimicrobial activity against enterovirulent bacteria. METHODS: Antibacterial activity was examined in vitro against a wide range of Gram negative and Gram positive pathogens. Inhibition of Salmonella typhimurium SL1334 cell association and cell invasion was investigated in vitro using Caco-2 cells. Colonisation of the gastrointestinal tract in vivo by bifidobacteria was examined in axenic C3/He/Oujco mice. Antimicrobial activity was examined in vivo in axenic C3/He/Oujco mice infected by the lethal S typhimurium C5 strain. RESULTS: Fourteen human bifidobacterium strains isolated from infant stools were examined for antimicrobial activity. Two strains (CA1 and F9) expressed antagonistic activity against pathogens in vitro, inhibited cell entry, and killed intracellular S typhimurium SL1344 in Caco-2 cells. An antibacterial component(s) produced by CA1 and F9 was found to be a lipophilic molecule(s) with a molecular weight of less than 3500. In the axenic C3/He/Oujco mice, CA1 and F9 strains colonised the intestinal tract and protected mice against S typhimurium C5 lethal infection. CONCLUSION: Several bifidobacterium strains from resident infant human gastrointestinal microflora exert antimicrobial activity, suggesting that they could participate in the "barrier effect" produced by the indigenous microflora.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1136/gut.47.5.646

Cites

52 of 52 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Bifidobacteria have been linked to strengthening gut mucosal protection through activities against pathogens.

    “these bacteria have been linked to fortification of the gut mucosal protection through activities against pathogens12, 13.”
  • Probiotics can provide twofold protection against a broad range of pathogens, including certain forms of Clostridium, Escherichia coli, Salmonella, Shigella and Pseudomonas, by competing for nutrients and producing metabolites and soluble factors inhibitory for some pathogenic bacteria.

    “As bacteria compete for nutrients and produce substances that directly affect the growth of other bacteria, probiotics can provide a twofold protection against a broad range of pathogens, including certain forms of Clostridium, Escherichia coli, Salmonella, Shigella and Pseudomonas”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

2 of the 54 references couldn’t be matched to a known paper.

Research works printed but not linked: 4

  1. Bernet M-F, Brassart D, Neeser JR, et al. Adhering bifidobacterial strains inhibit interactions of enteropatho- gens with cultured human intestinal epithelial cells. Appl Environ Microbiol 1993;59:4121-8.
  2. Bernet-Camard M-F, Liévin V, Brassart D, et al. The probi- otic human Lactobacillus acidophilus strain La1 secretes antibacterial substances active in vitro and in vivo. Appl Environ Microbiol 1997;63:2747-53.
  3. Chauvière G, Coconnier M-H, Kernéis S, et al. Competitive exclusion of diarrheagenic Escherichia coli (ETEC) from enterocyte-like Caco-2 cells in culture. FEMS Microbiol Lett 1992;91:213-18.
  4. Moyen EN, Bonneville F, Fauchère JL. Modification of intestinal colonization and translocation of Campylobacter jejuni in gnotobiotic mice by erythromycin or heat-killed Lactobacillus. Ann Inst Pasteur 1986;137:199-207.

Cited by

Sorted by citations

Authors
Liévin, V, Peiffer, I., Hudault, S., Rochat, F., Brassart, D., Neeser, J.-R., & Servin, A. L.
Affiliations
  • aInstitut National de la Santé et de la Recherche Médicale, Unité 510, UFR de Pharmacie, Université Paris XI, 92296 Châtenay-Malabry, France, bNestlé Research Centre, Vers-chez-les-blanc, BP 44, CH-1000 Lausanne 26, Switzerland
  • Inserm (FR)
Corresponding author
Servin, A L
Venue
Gut
Publisher
BMJ
Published
2000-11-01
Volume
47
Issue
5
Pages
646-652
ISSN
  • 0017-5749 (ISSN-L)
  • 1468-3288
OpenAlex
W2110840202
Cited by
397
References
54
Citations by year
2026: 10 · 2025: 5 · 2024: 18 · 2023: 17 · 2022: 14 · 2021: 20 · 2020: 14 · 2019: 16 · 2018: 17 · 2017: 11 · 2016: 24 · 2015: 19 · 2014: 23 · 2013: 18 · 2012: 16
FWCI
12.76
Type
journal article
Language
en
Open access
Locations
  • Gut (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
  • HighWire Press Open Archive (submittedVersion)
Funders
  • Institut National de la Santé et de la Recherche Médicale
  • Nestec
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
Keywords
Bifidobacterium, antimicrobial activity, infant gut microbiota, Salmonella typhimurium, cell invasion inhibition, Caco-2 cells, intestinal colonization, germ-free mice, probiotic bacteria
MeSH terms
Animals, Bacteriolysis, Bifidobacterium, Escherichia coli, Feces, Humans, Infant, Klebsiella pneumoniae, Listeria monocytogenes, Mice, Inbred C3H, Pseudomonas aeruginosa, Salmonella typhimurium, Shigella flexneri, Staphylococcus aureus, Streptococcus, Clostridioides difficile, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review