Molecular Description and Industrial Potential of Tn 6098 Conjugative Transfer Conferring Alpha-Galactoside Metabolism in Lactococcus lactis
Open accessAbstract
A novel 51-kb conjugative transposon of Lactococcus lactis , designated Tn 6098 , encoding the capacity to utilize α-galactosides such as raffinose and stachyose, was identified and characterized. Alpha-galactosides are a dominant carbon source in many plant-derived foods. Most dairy lactococcus strains are unable to use α-galactosides as a growth substrate, yet many of these strains are known to have beneficial industrial traits. Conjugal transfer of Tn 6098 was demonstrated from the plant-derived donor strain L. lactis KF147 to the recipient L. lactis NZ4501, a derivative of the dairy model strain L. lactis MG1363. The integration of Tn 6098 into the genome of the recipient strain was confirmed by Illumina sequencing of the transconjugant L. lactis NIZO3921. The molecular structure of the integration site was confirmed by a PCR product spanning the insertion site. A 15-bp direct repeat sequence (TTATACCATAATTAC) is present on either side of Tn 6098 in the chromosome of L. lactis KF147. One copy of this sequence is also present in the L. lactis MG1363 chromosome and represents the sole integration site. Phenotypic characterization of all strains showed that the transconjugant has not only acquired the ability to grow well in soy milk, a substrate rich in α-galactosides, but also has retained the flavor-forming capabilities of the recipient strain L. lactis MG1363. This study demonstrates how (induced) conjugation can be used to exploit the beneficial industrial traits of industrial dairy lactic acid bacteria in fermentation of plant-derived substrates.
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In some eubacteria and archaea, transposon-mediated LGT usually generates a pair of DRs varying from several to a dozen base pairs flanking the foreign gene after its integration into the host genome.
“In some eubacteria and archaea, such as Acinetobacter baumannii, Escherichia coli, Enterococcus faecium, Lactococcus lactis, and Mycobacterium smegmatis, transposon-mediated LGT usually generates a pair of DRs varying from several to a dozen base pairs flanking the foreign gene after its integration into the host genome.33–36”
From Whole genomic DNA sequencing and comparative genomic analysis of<i>Arthrospira platensis</i>: high genome plasticity and genetic diversity (2016) · cites this paper as [35]
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- Authors
- Machielsen, R., Siezen, R. J., van Hijum, S. A. F. T., & van Hylckama Vlieg, J. E. T.
- Affiliations
- Nederlands Instituut Voor Zuivel Oonderzoek (NL)
- Netherlands Bioinformatics Centre (NL)
- Top Institute Food and Nutrition, P.O. Box 557, 6700AN Wageningen, Netherlands
- Corresponding author
- Siezen, Roland J.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2011-01-15
- Volume
- 77
- Issue
- 2
- Pages
- 555-563
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC3020558
- OpenAlex
- W2140711310
- Cited by
- 34
- References
- 44
- Citations by year
- 2024: 2 · 2022: 1 · 2020: 1 · 2019: 5 · 2018: 2 · 2017: 3 · 2016: 1 · 2015: 2 · 2014: 4 · 2013: 3 · 2012: 1
- FWCI
- 6.38
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Radboud Repository (Radboud University) (submittedVersion)
- Research Repository (Delft University of Technology) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Keywords
- conjugative transposons, Lactococcus lactis, raffinose, stachyose, conjugative transfer, transconjugation, insertion site, direct repeats, soy milk fermentation, dairy lactic acid bacteria, flavor formation
- MeSH terms
- Conjugation, Genetic, DNA Transposable Elements, DNA, Bacterial, Galactosides, Industrial Microbiology, Recombination, Genetic, Lactococcus lactis, Polymerase Chain Reaction, Sequence Analysis, DNA, Food Industry, Gene Transfer, Horizontal, Soy Milk
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