Random Mutagenesis of Clostridium cellulolyticum by Using a Tn 1545 Derivative
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Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn1545-derived delivery tool which allows monocopy random insertion within the genome.
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Recent developments have seen the successful generation of mono-copy random insertion of transposon Tn1545 into C. cellulolyticum and mariner transposon Himar1 into C. difficile.
“Recent developments have seen the successful generation of mono-copy random insertion of transposon Tn1545 into C. cellulolyticum (Blouzard et al., 2010) and mariner transposon Himar1 into C. difficile (Cartman and Minton, 2010; Zhang et al., 2015b).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Blouzard et al., 2010
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Liew, F., Martin, M. E., Tappel, R. C., Heijstra, B. D., Mihalcea, C., & Köpke, M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00694 · Cited by 485 · see citing works
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Targeted gene engineering in Clostridium cellulolyticum H10 without methylation
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- Authors
- Blouzard, J.-C., Valette, O., Tardif, C., & de Philip, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Laboratoire de Chimie Bactérienne, IFR88-CNRS, Marseille, France
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-07
- Volume
- 76
- Issue
- 13
- Pages
- 4546-4549
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2897430
- OpenAlex
- W2098524970
- Cited by
- 35
- References
- 15
- Citations by year
- 2026: 1 · 2024: 3 · 2023: 2 · 2022: 12 · 2021: 2 · 2020: 2 · 2018: 1 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 2 · 2012: 3
- FWCI
- 1.08
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Clostridium cellulolyticum, random mutagenesis, transposon insertion, plant cell wall degradation, metabolic engineering
- MeSH terms
- DNA Transposable Elements, DNA, Bacterial, Genetic Engineering, Genetic Vectors, Mutagenesis, Insertional, Integrases, Clostridium cellulolyticum, Ruminiclostridium cellulolyticum
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