Distribution of nitrogen-13 from labeled nitrate and nitrite in germfree and conventional-flora rats
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The in vivo distribution of physiological concentrations of NO3- and NO2- labeled with 13N was studied in germfree and conventional-flora Sprague-Dawley rats after gastric intubation (gavage), intravenous (cardiac or tail vein), or intraluminal (intestinal) injection. Some in vitro studies were performed to determine the influence of the bacterial flora on ion distribution. After gavage with 13NO3-, essentially all of the label passed into the upper small intestine, where most was absorbed; however, up to 24% of the 13N could reach the ileum within 1 h. Gavage with 13NO2- resulted in some gastric absorption of the label, but most seemed to exit the stomach via passage into the duodenum. The exit of 13NO2- from the stomach was slower, and less 13N appeared to be absorbed from the small intestine than with 13NO3-. Movement of label through the gastrointestinal tract could be enhanced by inducing diarrhea. Absorbed 13N was either excreted in the urine, reentered the gastrointestinal tract at various points, or was temporarily stored in the eviscerated carcass. The bacterial flora, either by incorporation or chemical alteration, appeared to have some influence on the distribution of 13N from 13NO3- or 13NO2-.
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- Authors
- Witter, J. P., Balish, E., & Gatley, S. J.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1979-11
- Volume
- 38
- Issue
- 5
- Pages
- 870-878
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC243601
- OpenAlex
- W1819982032
- Cited by
- 45
- References
- 27
- Citations by year
- 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2018: 1 · 2016: 1 · 2014: 1
- FWCI
- 2.13
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- nitrogen-13, nitrate, nitrite, germ-free rats, gut microbiota, gastrointestinal absorption, intestinal transit, gavage, intravenous injection, diarrhea, urinary excretion
- MeSH terms
- Animals, Bacteria, Diarrhea, Digestive System, Feces, Germ-Free Life, Injections, Intravenous, Intubation, Gastrointestinal, Nitrates, Nitrites, Nitrogen Radioisotopes, Organ Specificity, Saliva, Sodium Nitrite, Urinary Tract, Rats, In Vitro Techniques
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