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Expression of the Escherichia coli xylose isomerase gene in Saccharomyces cerevisiae
Open accessAbstract
Transformation of Saccharomyces cerevisiae by yeast expression plasmids bearing the Escherichia coli xylose isomerase gene leads to production of the protein. Western blotting (immunoblotting) experiments show that immunoreactive protein chains which comigrate with the E. coli enzyme are made in the transformant strains and that the amount produced parallels the copy number of the plasmid. When comparable amounts of immunologically cross-reactive xylose isomerase protein made in E. coli or S. cerevisiae were assayed for enzymatic activity, however, the yeast protein was at least 10(3)-fold less active.
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Citations
Cited by
Metabolic engineering for improved fermentation of pentoses by yeasts
Jeffries, T. W., & Jin, Y.-S. (2004) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-003-1450-0 · Cited by 499
Metabolic Engineering of Saccharomyces cerevisiae
Ostergaard, S., Olsson, L., & Nielsen, J. (2000) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.64.1.34-50.2000 · Cited by 462
Functional Expression of a Bacterial Xylose Isomerase in Saccharomyces cerevisiae
Brat, D., Boles, E., & Wiedemann, B. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.02522-08 · Cited by 291
Zhou, H., Cheng, J.-S., Wang, B. L., Fink, G. R., & Stephanopoulos, G. (2012) · Metabolic Engineering · doi:10.1016/j.ymben.2012.07.011 · Cited by 287
Metabolic Engineering for Pentose Utilization in Saccharomyces cerevisiae
Hahn-Hägerdal, B., Karhumaa, K., Jeppsson, M., & Gorwa-Grauslund, M. F. (2007) · Advances in Biochemical Engineering/Biotechnology · doi:10.1007/10_2007_062 · Cited by 280
Metadata
- Authors
- Sarthy, A. V., McConaughy, B. L., Lobo, Z., Sundstrom, J. A., Furlong, C. E., & Hall, B. D.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1987-09
- Volume
- 53
- Issue
- 9
- Pages
- 1996-2000
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1515252291
- Cited by
- 160
- References
- 0
- FWCI
- 1.19
- Type
- journal article
- Language
- en
- Open access
- green
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- Sarthy, A. V., McConaughy, B. L., Lobo, Z., Sundstrom, J. A., Furlong, C. E., & Hall, B. D. (1987). Expression of the Escherichia coli xylose isomerase gene in Saccharomyces cerevisiae. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.53.9.1996-2000.1987
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