The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Recovery of DNA from soils and sediments
Open accessAbstract
Experiments were performed to evaluate the effectiveness of two different methodological approaches for recovering DNA from soil and sediment bacterial communities: cell extraction followed by lysis and DNA recovery (cell extraction method) versus direct cell lysis and alkaline extraction to recover DNA (direct lysis method). Efficiency of DNA recovery by each method was determined by spectrophotometric absorbance and using a tritiated thymidine tracer. With both procedures, the use of polyvinylpolypyrrolidone was important for the removal of humic compounds to improve the purity of the recovered DNA; without extensive purification, various restriction enzymes failed to cut added target DNA. Milligram quantities of high-purity DNA were recovered from 100-g samples of both soils and sediments by the direct lysis method, which was a greater than 1-order-of-magnitude-higher yield than by the cell extraction method. The ratio of labeled thymidine to total DNA, however, was higher in the DNA recovered by the cell extraction method. than by the direct lysis method, suggesting that the DNA recovered by the cell extraction method came primarily from active bacterial cells, whereas that recovered by the direct lysis method may have contained DNA from other sources.
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Cites
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Self-citationDynamics of extracellular DNA in the marine environment
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Using ecological diversity measures with bacterial communities
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Remarkable archaeal diversity detected in aYellowstone National Park hot spring environment.
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Quantification of Bias Related to the Extraction of DNA Directly from Soils
Frostegård, Å., Courtois, S., Ramisse, V., Clerc, S., Bernillon, D., Le Gall, F., Jeannin, P., Nesme, X., & Simonet, P. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.12.5409-5420.1999 · Cited by 543
Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction
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Metadata
- Authors
- Steffan, R. J., Goksøyr, J., Bej, A. K., & Atlas, R. M.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1988-12
- Volume
- 54
- Issue
- 12
- Pages
- 2908-2915
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1571452544
- Cited by
- 477
- References
- 11
- FWCI
- 19.89
- Type
- journal article
- Language
- en
- Open access
- green
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- Steffan, R. J., Goksøyr, J., Bej, A. K., & Atlas, R. M. (1988). Recovery of DNA from soils and sediments. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.54.12.2908-2915.1988
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
