The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Polymerase chain reaction detection of nonviable bacterial pathogens
Open accessAbstract
Polymerase chain reaction (PCR) methodologies for detection of pathogens in environmental samples are currently available. However, positive amplification products for any set of primers only signal that the appropriate target nucleic acid sequences were present in the sample. The presence of the amplification products does not imply that the target organisms were viable. Here we show that PCR will detect nonviable cells, as long as intact target nucleic acid sequences are available. In an environmental water sample, nucleic acids degraded quickly and were not detectable by PCR after 3 weeks even when stored at 4 degrees C. However, these data show that there is a window of opportunity for PCR analyses to result in false positives with respect to viable cells. We further show that care must be taken in the way samples are stored for future PCR amplifications and that filter sterilization of media is not acceptable for long-term preservation of samples for PCR.
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Cites
Survival strategies of bacteria in the natural environment
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A tentative direct microscopic method for counting living marine bacteria
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The extraction and purification of microbial DNA from sediments
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Differential amplification of rRNA genes by polymerase chain reaction
Reysenbach, A. L., Giver, L. J., Wickham, G. S., & Pace, N. R. (1992) · Applied and Environmental Microbiology · doi:10.1128/aem.58.10.3417-3418.1992 · Cited by 664
UV inactivation of pathogenic and indicator microorganisms
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Detection of coliform bacteria in water by polymerase chain reaction and gene probes
Bej, A. K., Steffan, R. J., DiCesare, J., Haff, L., & Atlas, R. M. (1990) · Applied and Environmental Microbiology · doi:10.1128/aem.56.2.307-314.1990 · Cited by 344
DNA amplification to enhance detection of genetically engineered bacteria in environmental samples
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Specific detection of Salmonella spp. by multiplex polymerase chain reaction
Way, J. S., Josephson, K. L., Pillai, S. D., Abbaszadegan, M., Gerba, C. P., & Pepper, I. L. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.5.1473-1479.1993 · Cited by 174
Self-citation
Cited by
Nocker, A., Cheung, C.-Y., & Camper, A. K. (2006) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2006.04.015 · Cited by 1,073
Chee‐Sanford, J. C., Mackie, R. I., Koike, S., Krapac, I. G., Lin, Y., Yannarell, A. C., Maxwell, S., & Aminov, R. I. (2009) · Journal of Environmental Quality · doi:10.2134/jeq2008.0128 · Cited by 830
Lee, C. S., Lee, S.-A., Ko, S.-R., Oh, H.-M., & Ahn, C.-Y. (2015) · Water Research · doi:10.1016/j.watres.2014.10.029 · Cited by 356
Molecular and Nonmolecular Diagnostic Methods for Invasive Fungal Infections
Arvanitis, M., Anagnostou, T., Fuchs, B. B., Caliendo, A. M., & Mylonakis, E. (2014) · Clinical Microbiology Reviews · doi:10.1128/cmr.00091-13 · Cited by 353
Colloid Transport in the Subsurface: Past, Present, and Future Challenges
McCarthy, J. F., & McKay, L. D. (2004) · Vadose Zone Journal · doi:10.2113/3.2.326 · Cited by 280
Metadata
- Authors
- Josephson, K. L., Gerba, C. P., & Pepper, I. L.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1993-10
- Volume
- 59
- Issue
- 10
- Pages
- 3513-3515
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1491378788
- Cited by
- 484
- References
- 9
- FWCI
- 20.84
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Josephson, K. L., Gerba, C. P., & Pepper, I. L. (1993). Polymerase chain reaction detection of nonviable bacterial pathogens. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.59.10.3513-3515.1993
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
