The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Differential amplification of rRNA genes by polymerase chain reaction
Open accessAbstract
The polymerase chain reaction (PCR) is used widely to recover rRNA genes from naturally occurring communities for analysis of population constituents. We have found that this method can result in differential amplification of different rRNA genes. In particular, rDNAs of extremely thermophilic archaebacteria often cannot be amplified by the usual PCR methods. The addition of 5% (wt/vol) acetamide to a PCR mixture containing both archaebacterial and yeast DNA templates minimized nonspecific annealing of the primers and prevented preferential amplification of the yeast small-subunit rRNA genes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Cited by
Bias in template-to-product ratios in multitemplate PCR
Polz, M. F., & Cavanaugh, C. M. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.10.3724-3730.1998 · Cited by 1,484
Kassinen, A., Krogius-Kurikka, L., Mäkivuokko, H., Rinttilä, T., Paulin, L., Corander, J., Malinen, E., Apajalahti, J., & Palva, A. (2007) · Gastroenterology · doi:10.1053/j.gastro.2007.04.005 · Cited by 1,002
PCR-DGGE fingerprinting: novel strategies for detection of microbes in food
Ercolini, D. (2004) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2003.11.006 · Cited by 624
Ercolini, D. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.00256-13 · Cited by 479
Effects of Amplification Facilitators on Diagnostic PCR in the Presence of Blood, Feces, and Meat
Abu Al-Soud, W., & Rådström, P. (2000) · Journal of Clinical Microbiology · doi:10.1128/jcm.38.12.4463-4470.2000 · Cited by 398
Metadata
- Authors
- Reysenbach, A. L., Giver, L. J., Wickham, G. S., & Pace, N. R.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1992-10
- Volume
- 58
- Issue
- 10
- Pages
- 3417-3418
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2159748283
- Cited by
- 664
- References
- 1
- FWCI
- 2.42
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Differential amplification of rRNA genes by polymerase chain reaction. https://app.cochat.ai/papers/10.1128/aem.58.10.3417-3418.1992
- The paper
- Reysenbach, A. L., Giver, L. J., Wickham, G. S., & Pace, N. R. (1992). Differential amplification of rRNA genes by polymerase chain reaction. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.58.10.3417-3418.1992
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
