Plasmid Transfer into the Homoacetogen Acetobacterium woodii by Electroporation and Conjugation

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Yanisch-Perron, C., Vieira, J., & Messing, J. W. (1985) · Gene · doi:10.1016/0378-1119(85)90120-9 · Cited by 14,868 · see citing works

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686 · see citing works

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

Simon, R., Priefer, U. B., & Pühler, A. (1983) · Nature Biotechnology · doi:10.1038/nbt1183-784 · Cited by 7,342 · see citing works

High efficiency transformation of E.coli by high voltage electroporation

Dower, W. J., Miller, J. F., & Ragsdale, C. W. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.13.6127 · Cited by 2,821 · see citing works

Simple and rapid method for isolating large plasmid DNA from lactic streptococci

Anderson, D. G., & McKay, L. L. (1983) · Applied and Environmental Microbiology · doi:10.1128/aem.46.3.549-552.1983 · Cited by 1,026 · see citing works

The Autotrophic Pathway of Acetate Synthesis in Acetogenic Bacteria

Ljungdhal, L. G. (1986) · Annual Review of Microbiology · doi:10.1146/annurev.mi.40.100186.002215 · Cited by 575 · see citing works

CO2 fixation in acetogenic bacteria: Variations on a theme

Fuchs, G. (1986) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1986.tb01859.x · Cited by 294 · see citing works

Plasmid transfer by conjugation from Escherichia coli to Gram-positive bacteria

Trieu‐Cuot, P., Carlier, C., Martin, P., & Courvalin, P. M. (1987) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1987.tb02558.x · Cited by 290 · see citing works

Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria

Gawron-Burke, C., & Clewell, D. B. (1984) · Journal of Bacteriology · doi:10.1128/jb.159.1.214-221.1984 · Cited by 262 · see citing works

Enzymology of the Acetyl-CoA Pathway of CO2Fixation

Ragsdale, S. W. (1991) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409239109114070 · Cited by 259 · see citing works

Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824

Mermelstein, L. D., Welker, N. E., Bennett, G. N., & Papoutsakis, E. T. (1992) · Nature Biotechnology · doi:10.1038/nbt0292-190 · Cited by 249 · see citing works

Shuttle vectors containing a multiple cloning site and a lacZα gene for conjugal transfer of DNA from Escherichia coli to Gram-positive bacteria

Trieu‐Cuot, P., Carlier, C., Poyart, C., & Courvalin, P. M. (1991) · Gene · doi:10.1016/0378-1119(91)90546-n · Cited by 210 · see citing works

Life with CO or CO ₂ and H ₂ as a source of carbon and energy

Wood, H. G. (1991) · The FASEB Journal · doi:10.1096/fasebj.5.2.1900793 · Cited by 200 · see citing works

Conjugative plasmid transfer from Escherichia coli to Clostridium acetobutylicum

Williams, D. R., Young, D. I., & Young, M. (1990) · Journal of General Microbiology · doi:10.1099/00221287-136-5-819 · Cited by 171 · see citing works

Structurally stable Bacillus subtilis cloning vectors

Jannière, L., Bruand, C., & Ehrlich, S. D. (1990) · Gene · doi:10.1016/0378-1119(90)90495-d · Cited by 153 · see citing works

A pair of mobilizable shuttle vectors conferring resistance to spectinomycin for molecular cloning inEscherichia coliand in Gram-positive bacteria

Trieu‐Cuot, P., Carlier, C., Poyart, C., & Courvalin, P. M. (1990) · Nucleic Acids Research · doi:10.1093/nar/18.14.4296 · Cited by 153 · see citing works

Natural transfer of conjugative transposon Tn916 between gram-positive and gram-negative bacteria

Bertram, J., Strätz, M., & Dürre, P. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.2.443-448.1991 · Cited by 141 · see citing works

High efficiency introduction of plasmid DNA into glycine treated Enterococcus faecalis by electroporation

Cruz-Rodz, A. L., & Gilmore, M. S. (1990) · Molecular and General Genetics MGG · doi:10.1007/bf00259462 · Cited by 140 · see citing works

Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation

Oultram, J. D., Loughlin, M., Swinfield, T.-J., Brehm, J. K., Thompson, D. E., & Minton, N. P. (1988) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1988.tb03154.x · Cited by 133 · see citing works

Production and Utilization of Ethanol by the Homoacetogen Acetobacterium woodii

Buschhorn, H., Dürre, P., & Gottschalk, G. (1989) · Applied and Environmental Microbiology · doi:10.1128/aem.55.7.1835-1840.1989 · Cited by 93 · see citing works

Conjugal transfer and expression of streptococcal transposons in Clostridium acetobutylicum

Bertram, J., & D�rre, P. (1989) · Archives of Microbiology · doi:10.1007/bf00454874 · Cited by 77 · see citing works

Transformation of Heat-Treated Clostridium acetobutylicum Protoplasts with pUB110 Plasmid DNA

Lin, Y.-L., & Blaschek, H. P. (1984) · Applied and Environmental Microbiology · doi:10.1128/aem.48.4.737-742.1984 · Cited by 76 · see citing works

Molecular genetics of resistance to macrolides, lincosamides and streptogramin B (MLS) in streptococci

Horaud, T., Bouguénec, C. L., & Pepper, K. (1985) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/16.suppl_a.111 · Cited by 67 · see citing works

Conjugal transfer of plasmid pAMβ1 fromStreptococcus lactisandBacillus subtilistoClostridium acetobutylicum

Oultram, J. D., & Young, M. (1985) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1985.tb01652.x · Cited by 61 · see citing works

Construction of an Escherichia coli-Clostridium perfringens shuttle vector and plasmid transformation of Clostridium perfringens

Kim, A. Y., & Blaschek, H. P. (1989) · Applied and Environmental Microbiology · doi:10.1128/aem.55.2.360-365.1989 · Cited by 51 · see citing works

Purification and properties of the coenzyme A-linked acetaldehyde dehydrogenase of Acetobacterium woodii

Buschhorn, H., Dürre, P., & Gottschalk, G. (1992) · Archives of Microbiology · doi:10.1007/bf00245216 · Cited by 13 · see citing works

Transfer and expression of the tetracyclin resistance transposon Tn925inAcetobacterium woodii

Strätz, M., Gottschalk, G., & Dürre, P. (1990) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1990.tb04144.x · Cited by 12 · see citing works

Isolation and characterization of DNase-deficient mutants ofClostridium acetobutylicum

Burchhardt, G., & Dürre, P. (1990) · Current Microbiology · doi:10.1007/bf02092096 · Cited by 8 · see citing works

Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks

Liew, F., Martin, M. E., Tappel, R. C., Heijstra, B. D., Mihalcea, C., & Köpke, M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00694 · Cited by 485 · see citing works

Using gas mixtures of CO, CO 2 and H 2 as microbial substrates: the do's and don'ts of successful technology transfer from laboratory to production scale

Takors, R., Kopf, M., Mampel, J., Bluemke, W., Blombach, B., Eikmanns, B. J., Bengelsdorf, F. R., Weuster‐Botz, D., & Dürre, P. (2018) · Microbial Biotechnology · doi:10.1111/1751-7915.13270 · Cited by 192 · see citing works

Bacterial Anaerobic Synthesis Gas (Syngas) and CO 2 + H 2 Fermentation

Bengelsdorf, F. R., Beck, M. H., Erz, C., Hoffmeister, S., Karl, M. M., Riegler, P., Wirth, S., Poehlein, A., Weuster‐Botz, D., & Dürre, P. (2018) · Advances in applied microbiology · doi:10.1016/bs.aambs.2018.01.002 · Cited by 164 · see citing works

Selective enhancement of autotrophic acetate production with genetically modified Acetobacterium woodii

Straub, M., Demler, M., Weuster-Botz, D., & Dürre, P. (2014) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2014.03.005 · Cited by 162 · see citing works

Acetone production with metabolically engineered strains of Acetobacterium woodii

Hoffmeister, S., Gerdom, M., Bengelsdorf, F. R., Linder, S., Flüchter, S., Öztürk, H., Blümke, W., May, A., Fischer, R.-J., Bahl, H., et al. (2016) · Metabolic Engineering · doi:10.1016/j.ymben.2016.03.001 · Cited by 139 · see citing works

Adaptation of the autotrophic acetogen Sporomusa ovata to methanol accelerates the conversion of CO2 to organic products

Tremblay, P., Höglund, D., Koza, A., Bonde, I., & Zhang, T. (2015) · Scientific Reports · doi:10.1038/srep16168 · Cited by 122 · see citing works

Acetogenic mixotrophy: novel options for yield improvement in biofuels and biochemicals production

Fast, A. G., Schmidt, E. D., Jones, S. W., & Tracy, B. P. (2014) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2014.11.014 · Cited by 117 · see citing works

Gas fermentation: cellular engineering possibilities and scale up

Heijstra, B. D., Leang, C., & Juminaga, A. (2017) · Microbial Cell Factories · doi:10.1186/s12934-017-0676-y · Cited by 92 · see citing works

A Simple and Rapid Method of Transformation of Streptomyces rimosus R6 and Other Streptomycetes by Electroporation

Pigac, J., & Schrempf, H. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.1.352-356.1995 · Cited by 81 · see citing works

Genetic and metabolic engineering challenges of C1-gas fermenting acetogenic chassis organisms

Bourgade, B., Minton, N. P., & Islam, M. A. (2021) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuab008 · Cited by 66 · see citing works

Development of a metabolic pathway transfer and genomic integration system for the syngas-fermenting bacterium Clostridium ljungdahlii

Philipps, G., de Vries, S., & Jennewein, S. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1448-1 · Cited by 54 · see citing works

Advanced aspects of acetogens

Poehlein, A., Zeldes, B. M., Flaiz, M., Böer, T., Lüschen, A., Höfele, F., Baur, K. S., Molitor, B., Kröly, C., Wang, M., et al. (2024) · Bioresource Technology · doi:10.1016/j.biortech.2024.131913 · Cited by 51 · see citing works

Syngas Biorefinery and Syngas Utilization

Tissera, S. L. D., Köpke, M., Simpson, S. D., Humphreys, C. M., Minton, N. P., & Dürre, P. (2017) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2017_5 · Cited by 50 · see citing works

Microbial Production of Acetone/Butanol/Isopropanol

Dürre, P., & Bahl, H. (1996) · Biotechnology · doi:10.1002/9783527620883.ch6 · Cited by 46 · see citing works

Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis

Rachinger, M., Bauch, M., Strittmatter, A. W., Bongaerts, J., Evers, S., Maurer, K., Daniel, R., Liebl, W., Liesegang, H., & Ehrenreich, A. (2013) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2013.07.026 · Cited by 30 · see citing works

Acetogens: Biochemistry, Bioenergetics, Genetics, and Biotechnological Potential

Debabov, V. G. (2021) · Microbiology · doi:10.1134/s0026261721030024 · Cited by 30 · see citing works

Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals: current status and future perspectives

Wang, J., Qin, R., Guo, Y., Ma, C., Wang, X., Chen, K., & Ouyang, P. (2022) · Green Chemical Engineering · doi:10.1016/j.gce.2022.10.005 · Cited by 28 · see citing works

An improved transconjugation protocol for Bacillus megaterium facilitating a direct genetic knockout

Richhardt, J., Larsen, M., & Meinhardt, F. (2010) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-010-2503-9 · Cited by 18 · see citing works

Engineering acetogens for biofuel production: From cellular biology to process improvement

Yang, C., Dong, L., Gao, Y., Jia, P., & Diao, Q. (2021) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2021.111563 · Cited by 14 · see citing works

Lactate based caproate production with Clostridium drakei and process control of Acetobacterium woodii via lactate dependent in situ electrolysis

Herzog, J., Mook, A., Utesch, T., Bengelsdorf, F. R., & Zeng, A. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1212044 · Cited by 10 · see citing works

Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways

Moreira, J. P. C., Heap, J. T., Alves, J. I., & Domingues, L. (2023) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-023-02259-6 · Cited by 10 · see citing works

Utilization of the liquid one carbon feedstocks methanol and formate for acetogenic bioproduction of chemicals and fuels

Pettinato, E., Hocq, R., & Pflügl, S. (2025) · Bioresource Technology · doi:10.1016/j.biortech.2025.132643 · Cited by 7 · see citing works

Acetogenic Bacteria for Biotechnological Applications

Litty, D., & Müller, V. (2020) · Springer eBooks · doi:10.1007/978-3-030-58315-6_4 · Cited by 6 · see citing works

Lessons from Clostridial Genetics: Toward Engineering Acetogenic Bacteria

Lee, J. (2021) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-021-0062-9 · Cited by 6 · see citing works

1 Introduction of DNA into Bacteria

Saunders, J. R., & Saunders, V. A. (1999) · Methods in microbiology · doi:10.1016/s0580-9517(08)70112-0 · Cited by 6 · see citing works

Microbial Production of Acetone/Butanol/Isopropanol

Dürre, P., & Bahl, H. (2001) · Biotechnology Set · doi:10.1002/9783527620999.ch6f · Cited by 2 · see citing works

The defining genomic and predicted metabolic features of the Acetobacterium genusPreprint

Ross, D. E., Marshall, C. W., Gulliver, D. M., May, H. D., & Norman, R. S. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.01.23.904417 · Cited by 2 · see citing works

A Novel Approach to Managing Invasive Termite Species Using Genetically Engineered Bacteria

Husseneder, C., Collier, R., & Delatte, J. R. (2008) · doi:10.21236/ada495508

Biokatalytische Konversion

Bengelsdorf, F. R., & Dürre, P. (2020) · Springer eBooks · doi:10.1007/978-3-662-60649-0_7

Microbial Processes: Biocatalytic Conversion

Dürre, P., & Bengelsdorf, F. R. (2023) · Circular economy and sustainability · doi:10.1007/978-3-031-27811-2_7

Amazing attributes of acetogensPreprint

Poehlein, A., Zeldes, B. M., Flaiz, M., Böer, T., Lüschen, A., Höfele, F., Baur, K. S., Molitor, B., Kröly, C., Wang, M., et al. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4872466

Additional file 1 of Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways

Moreira, J. P. C., Heap, J. T., Alves, J. I., & Domingues, L. (2024) · Figshare · doi:10.6084/m9.figshare.26568691

A characterized promoter suite enables metabolic rewiring of the acetogen Acetobacterium woodii toward ethanol production

Eynard, R., Justine, S., Hocq, R., & Pflügl, S. (2026) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-026-02790-2

Conjugative plasmid transfer from Escherichia coli to Clostridium acetobutylicum

Williams, D. R., Young, D. I., & Young, M. (1990) · Journal of General Microbiology · doi:10.1099/00221287-136-5-819 · Cited by 171 · see citing works

A Host–Vector System for a Cellulose-ProducingAcetobacterStrain

Tonouchi, N., Tsuchida, T., Yoshinaga, F., Horinouchi, S., & Beppu, T. (1994) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.58.1899 · Cited by 29 · see citing works

Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation

Oultram, J. D., Loughlin, M., Swinfield, T.-J., Brehm, J. K., Thompson, D. E., & Minton, N. P. (1988) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1988.tb03154.x · Cited by 133 · see citing works

Shuttle vectors containing a multiple cloning site and a lacZα gene for conjugal transfer of DNA from Escherichia coli to Gram-positive bacteria

Trieu‐Cuot, P., Carlier, C., Poyart, C., & Courvalin, P. M. (1991) · Gene · doi:10.1016/0378-1119(91)90546-n · Cited by 210 · see citing works

Plasmid transfer by conjugation from Escherichia coli to Gram-positive bacteria

Trieu‐Cuot, P., Carlier, C., Martin, P., & Courvalin, P. M. (1987) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1987.tb02558.x · Cited by 290 · see citing works