Selective enhancement of autotrophic acetate production with genetically modified Acetobacterium woodii
Abstract
Great interest has emerged in the recent past towards the potential of autotrophic acetogenic bacteria for the sustainable production of fuels and chemicals. This group of microorganisms possesses an ancient pathway for the fixation of carbon dioxide in the presence of hydrogen, making them highly attractive for the utilization of gas mixtures as a cheap and abundant carbon and energy source. As more and more genome sequence data of acetogens becomes available, the genetic tools are being developed concomitantly. Here, we demonstrate for the first time the genetic modification of the well-characterized acetogen Acetobacterium woodii. This microorganism selectively produces acetate under autotrophic conditions, but seems to be limited at high acetate concentrations. To increase the carbon flow through the Wood-Ljungdahl pathway and therefore increase the efficiency of CO2 fixation, genes of enzyme groups of this pathway were selectively overexpressed (the four THF-dependent enzymes for the processing of formate as well as phosphotransacetylase and acetate kinase to enhance an ATP-generation step). Acetate production with genetically modified strains was increased in a batch process under pH-controlled reaction conditions in a stirred-tank reactor with continuous sparging of H2 and CO2. Final acetate concentrations of more than 50gL(-1) acetate were thus measured with the recombinant strains at low cell concentrations of 1.5-2gL(-1) dry cell mass in less than four days under autotrophic conditions.
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In A. woodii, plasmid-based expression of formyl-THF-synthetase, methenyl-THF-cyclohydrolase, methylene-THF-dehydrogenase, and methylene-THF-reductase of C. ljungdahlii increased volumetric acetate production rate by 14% under H2/CO2 conditions in CSTR.
“| A. woodii | Plasmid based expression of formyl-THF-synthetase, methenyl-THF-cyclohydrolase, methylene-THF-dehydrogenase, and methylene-THF-reductase of C. ljungdahlii | Increase in volumetric acetate production rate by 14% under H2/CO2 conditions in CSTR | Straub et al., 2014 |”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Straub et al., 2014
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- Authors
- Straub, M., Demler, M., Weuster-Botz, D., & Dürre, P.
- Affiliations
- Universität Ulm (DE)
- Technical University of Munich (DE)
- Corresponding author
- Straub, Melanie
- ORCID
- Dürre, Peter
- Venue
- Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2014-05
- Volume
- 178
- Pages
- 67-72
- ISSN
- 0168-1656 (ISSN-L)
- OpenAlex
- W1979827936
- Cited by
- 162
- References
- 29
- Citations by year
- 2026: 11 · 2025: 15 · 2024: 18 · 2023: 17 · 2022: 18 · 2021: 16 · 2020: 14 · 2019: 7 · 2018: 14 · 2017: 10 · 2016: 8 · 2015: 10 · 2014: 4
- FWCI
- 4.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wacker Chemie AG (Munich, Germany)
- Federal Ministry of Education and Research (BMBF, Berlin, Germany) (0315192, 0315192 and CLIB2021, 031A100B)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Keywords
- Acetobacterium woodii, acetate production, Wood-Ljungdahl pathway, genetic modification, gene overexpression, phosphotransacetylase, acetate kinase, CO2 fixation, hydrogen, bioprocess scale-up
- MeSH terms
- Acetate Kinase, Acetates, Carbon Dioxide, Fructose, Hydrogen, Bioreactors, Acetobacterium, Metabolic Engineering
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