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Cited by 266

The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.

A nitrite microsensor for profiling environmental biofilms

Open access
De Beer, D, Schramm, A, Santegoeds, C M, Kuhl, M·1997·Applied and Environmental Microbiology

Abstract

A highly selective liquid membrane nitrite microsensor based on the hydrophobic ion-carrier aquocyanocobalt(III)-hepta(2-phenylethyl)-cobrynate is described. The sensor has a tip diameter of 10 to 15 (mu)m. The response is log-linear in freshwater down to 1 (mu)M NO(inf2)(sup-) and in seawater to 10 (mu)M NO(inf2)(sup-). A method is described for preparation of relatively large polyvinyl chloride (PVC)-gelled liquid membrane microsensors with a tip diameter of 5 to 15 (mu)m, having a hydrophilic coating on the tip. The coating and increased tip diameter resulted in more sturdy sensors, with a lower detection limit and a more stable signal than uncoated nitrite sensors with a tip diameter of 1 to 3 (mu)m. The coating protects the sensor membrane from detrimental direct contact with biomass and can be used for all PVC-gelled liquid membrane sensors meant for profiling microbial mats, biofilms, and sediments. Thanks to these improvements, liquid membrane sensors can now be used in complex environmental samples and in situ, e.g., in operating bioreactors. Examples of measurements in denitrifying, nitrifying, and nitrifying/denitrifying biofilms from wastewater treatment plants are shown. In all of these biofilms high nitrite concentrations were found in narrow zones of less than 1 mm.

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Crossref · 10.1128/aem.63.3.973-977.1997

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Metadata

Authors
De Beer, D., Schramm, A., Santegoeds, C. M., & Kuhl, M.
Venue
Applied and Environmental Microbiology
Published
1997-03
Volume
63
Issue
3
Pages
973-977
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2170788996
Cited by
266
References
16
FWCI
9.13
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: A nitrite microsensor for profiling environmental biofilms. https://app.cochat.ai/papers/10.1128/aem.63.3.973-977.1997
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De Beer, D., Schramm, A., Santegoeds, C. M., & Kuhl, M. (1997). A nitrite microsensor for profiling environmental biofilms. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.63.3.973-977.1997

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.