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Plasposons: Modular Self-Cloning Minitransposon Derivatives for Rapid Genetic Analysis of Gram-Negative Bacterial Genomes
Open accessAbstract
A series of modular mini-transposon derivatives which permit the rapid cloning and mapping of the DNA flanking the minitransposon’s site of insertion has been developed. The basic plasposon, named Tn Mod , consists of the Tn 5 inverted repeats, a conditional origin of replication, rare restriction endonuclease multiple cloning sites, and exchangeable antibiotic resistance cassettes. The broad host range and low target DNA sequence specificity of the Tn 5 transposase, in combination with the flexibility afforded by the modular arrangement of Tn Mod , result in a versatile tool for the mapping of insertional mutations and the rapid recovery of clones from gram-negative bacteria.
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GABA controls the level of quorum-sensing signal in Agrobacterium tumefaciens
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Metadata
- Authors
- Dennis, J. J., & Zylstra, G. J.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1998-07
- Volume
- 64
- Issue
- 7
- Pages
- 2710-2715
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2121178227
- Cited by
- 473
- References
- 24
- FWCI
- 4.36
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Plasposons: Modular Self-Cloning Minitransposon Derivatives for Rapid Genetic Analysis of Gram-Negative Bacterial Genomes. https://app.cochat.ai/papers/10.1128/aem.64.7.2710-2715.1998
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- Dennis, J. J., & Zylstra, G. J. (1998). Plasposons: Modular Self-Cloning Minitransposon Derivatives for Rapid Genetic Analysis of Gram-Negative Bacterial Genomes. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.64.7.2710-2715.1998
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
