Cited by 203Aquaculture disease management and microbiotaVibrio bacteria research studiesCrustacean biology and ecology

Vibrios Associated with Litopenaeus vannamei Larvae, Postlarvae, Broodstock, and Hatchery Probionts

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Vandenberghe, Johan, Verdonck, Linda, Robles-Arozarena, Rocio, Rivera, Gabriel, Bolland, Annick, Balladares, Marcos, Gomez-Gil, Bruno, Calderon, Jorge +2 more·1999·Applied and Environmental Microbiology

Abstract

Several bacteriological surveys were performed from 1994 to 1996 at different Litopenaeus vannamei hatcheries (in Ecuador) and shrimp farms (in Mexico). Samples were taken from routine productions of healthy and diseased L. vannamei larvae, postlarvae, and their culture environment and from healthy and diseased juveniles and broodstock. In Ecuador, the dominant bacterial flora associated with shrimp larvae showing symptoms of zoea 2 syndrome, mysis mold syndrome, and bolitas syndrome has been determined. Strains were characterized by Biolog metabolic fingerprinting and identified by comparison to a database of 850 Vibrio type and reference strains. A selection of strains was further genotypically fine typed by AFLP. Vibrio alginolyticus is predominantly present in all larval stages and is associated with healthy nauplius and zoea stages. AFLP genetic fingerprinting shows high genetic heterogeneity among V. alginolyticus strains, and the results suggest that putative probiotic and pathogenic strains each have specific genotypes. V. alginolyticus was found to be associated with larvae with the zoea 2 syndrome and the mysis mold syndrome, while different Vibrio species (V. alginolyticus and V. harveyi) are associated with the bolitas syndrome. V. harveyi is associated with diseased postlarvae, juveniles, and broodstock. The identities of the strains identified as V. harveyi by the Biolog system could not be unambiguously confirmed by AFLP genomic fingerprinting. Vibrio strain STD3-988 and one unidentified strain (STD3-959) are suspected pathogens of only juvenile and adult stages. V. parahaemolyticus, Photobacterium damselae, and V. mimicus are associated with juvenile and adult stages.

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Crossref · 10.1128/aem.65.6.2592-2597.1999

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Research works printed but not linked: 3

  1. In P. V. McVey (ed.), CRC handbook of mariculture. CRC Press, Boca Raton, Fla.
  2. In M. Shariff, R. P. Subasinghe, and J. R. Arthur (ed.), Diseases in Asian aquaculture I. Fish Health Section, Asian Fisheries Society, Manila, The Philippines.
  3. Owens, L., P. Muir, D. Sutton, and M. Wingfield. 1992. The pathology of microbial diseases in tropical Australian Crustacea, p. 165-172. In M. Shariff, R. P. Subasinghe, and J. R. Arthur (ed.), Diseases in Asian aquaculture I. Fish Health Section, Asian Fisheries Society, Manila, The Philippines.

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Authors
Vandenberghe, J., Verdonck, L., Robles-Arozarena, R., Rivera, G., Bolland, A., Balladares, M., Gomez-Gil, B., Calderon, J., Sorgeloos, P., & Swings, J.
Affiliations
  • Ghent University (BE)
  • <!--label omitted: 1-->Laboratory for Microbiology1 and
  • <!--label omitted: 3-->Centro Nacional de Acuicultura e Investigaciones Marinas “Edgar Arellano M.” CENAIM, Campus Politécnico (ESPOL), Guayaquil, Ecuador3; and
  • <!--label omitted: 4-->CIAD/Mazatlan Unit for Aquaculture and Environmental Management, Mazatlan, Sin., Mexico 820004
Venue
Applied and Environmental Microbiology
Published
1999-06
Volume
65
Issue
6
Pages
2592-2597
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2102804042
Cited by
203
References
21
Citations by year
2026: 5 · 2025: 8 · 2024: 4 · 2023: 13 · 2022: 7 · 2021: 23 · 2020: 16 · 2019: 5 · 2018: 7 · 2017: 4 · 2016: 2 · 2015: 15 · 2014: 11 · 2013: 5 · 2012: 9
FWCI
0.92
Type
journal article
Language
en
Open access
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion, other-oa)
  • Flanders Marine Institute (Flanders Marine Institute) (submittedVersion)
Funders
European Commission
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Vibrio bacteria research studies
  • Physical Sciences › Environmental Science › Ecology › Crustacean biology and ecology
Keywords
Vibrio, Litopenaeus vannamei, shrimp larvae, postlarvae, broodstock, Vibrio alginolyticus, Vibrio harveyi, Vibrio parahaemolyticus, Photobacterium damselae, Vibrio mimicus, bacterial pathogenesis, AFLP genotyping, shrimp aquaculture
MeSH terms
Animals, DNA Restriction Enzymes, Larva, Photobacterium, Genetic Variation, Vibrio, DNA Fingerprinting, Aquaculture, Penaeidae

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