Cited by 222Aquaculture disease management and microbiotaGut microbiota and healthInvertebrate Immune Response Mechanisms

Bacterial dynamics in intestines of the black tiger shrimp and the Pacific white shrimp during Vibrio harveyi exposure

Rungrassamee, Wanilada, Klanchui, Amornpan, Maibunkaew, Sawarot, Karoonuthaisiri, Nitsara·2016·Journal of Invertebrate Pathology

Abstract

The intestinal microbiota play important roles in health of their host, contributing to maintaining the balance and resilience against pathogen. To investigate effects of pathogen to intestinal microbiota, the bacterial dynamics upon a shrimp pathogen, Vibrio harveyi, exposures were determined in two economically important shrimp species; the black tiger shrimp (BT) and the Pacific white shrimp (PW). Both shrimp species were reared under the same diet and environmental conditions. Shrimp survival rates after the V. harveyi exposure revealed that the PW shrimp had a higher resistance to the pathogen than the BT shrimp. The intestinal bacterial profiles were determined by denaturing gradient gel electrophoresis (DGGE) and barcoded pyrosequencing of the 16S rRNA sequences under no pathogen challenge control and under pathogenic V. harveyi challenge. The DGGE profiles showed that the presence of V. harveyi altered the intestinal bacterial patterns in comparison to the control in BT and PW intestines. This implies that bacterial balance in shrimp intestines was disrupted in the presence of V. harveyi. The barcoded pyrosequencing analysis showed the similar bacterial community structures in intestines of BT and PW shrimp under a normal condition. However, during the time course exposure to V. harveyi, the relative abundance of bacteria belong to Vibrio genus was higher in the BT intestines at 12h after the exposure, whereas relative abundance of vibrios was more stable in PW intestines. The principle coordinates analysis based on weighted-UniFrac analysis showed that intestinal bacterial population in the BT shrimp lost their ability to restore their bacterial balance during the 72-h period of exposure to the pathogen, while the PW shrimp were able to reestablish their bacterial population to resemble those seen in the unexposed control group. This observation of bacterial disruption might correlate to different mortality rates observed between the two shrimp species. Our findings provide evidence of intestinal bacterial population altered by a presence of the pathogen in shrimp intestines and intestinal bacterial stability might provide colonization resistance against the invading pathogen in the host shrimp. Hence, intestinal microbial ecology management may potentially contribute to disease prevention in aquaculture.

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Crossref · 10.1016/j.jip.2015.11.004

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Authors
Rungrassamee, W., Klanchui, A., Maibunkaew, S., & Karoonuthaisiri, N.
Affiliations
National Center for Genetic Engineering and Biotechnology (TH)
Corresponding author
Rungrassamee, Wanilada
Venue
Journal of Invertebrate Pathology
Publisher
Elsevier BV
Published
2016-01
Volume
133
Pages
12-19
ISSN
0022-2011 (ISSN-L)
OpenAlex
W2172537579
Cited by
222
References
31
Citations by year
2026: 10 · 2025: 17 · 2024: 27 · 2023: 33 · 2022: 27 · 2021: 23 · 2020: 26 · 2019: 14 · 2018: 29 · 2017: 9 · 2016: 6
FWCI
7.21
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Invertebrate Pathology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Thailand Research Fund (TRG5480014)
  • National Center for Genetic Engineering and Biotechnology (P-13-50417)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
Keywords
Vibrio harveyi, Penaeus monodon, Litopenaeus vannamei, gut microbiota, bacterial community dynamics, shrimp disease resistance, colonization resistance, shrimp mortality, aquaculture disease prevention, microbial ecology
MeSH terms
Gastrointestinal Microbiome, Animals, Vibrio, Penaeidae, Biodiversity, Disease Resistance

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