Engineering Desiccation Tolerance in Escherichia coli
Open accessAbstract
Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation over phosphorus pentoxide. The phase transition temperatures and vibration frequencies (PO stretch) in phospholipids suggested that sucrose maintained membrane fluidity during cell dehydration.
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Sucrose accumulation in cyanobacteria during conditions of salt stress or desiccation plays a fundamental role as an osmotic protective agent.
“Sucrose (α-D-glucopyranosyl β-D-fructofuranoside) accumulation in cyanobacteria during conditions of salt stress or desiccation plays a fundamental role as an osmotic protective agent (Billi et al. 2000; Miao et al. 2003a).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Billi et al. 2000
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Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., et al. (1996) · DNA Research · doi:10.1093/dnares/3.3.185 · Cited by 2,434 · see citing works
Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., et al. (1996) · DNA Research · doi:10.1093/dnares/3.3.109 · Cited by 2,132 · see citing works
Preservation of Membranes in Anhydrobiotic Organisms: The Role of Trehalose
Crowe, J. H., Crowe, L. M., & Chapman, D. (1984) · Science · doi:10.1126/science.223.4637.701 · Cited by 1,509 · see citing works
Self-citationDesiccation tolerance of prokaryotes
Potts, M. (1994) · Microbiological Reviews · doi:10.1128/mr.58.4.755-805.1994 · Cited by 1,124 · see citing works
Self-citationTrehalose and sucrose protect both membranes and proteins in intact bacteria during drying
Leslie, S., Israeli, E., Lighthart, B., Crowe, J. H., & Crowe, L. M. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.10.3592-3597.1995 · Cited by 978 · see citing works
Self-citationRevival and identification of bacterial spores in 25- to 40-million-year-old Dominican amber
Cano, R. J., & Borucki, M. K. (1995) · Science · doi:10.1126/science.7538699 · Cited by 641 · see citing works
Drobnis, E. Z., Crowe, L. M., Berger, T., et al. (1993) · Journal of Experimental Zoology · doi:10.1002/jez.1402650413 · Cited by 554 · see citing works
Holmström, K., Mäntylä, E., Welin, B. V., Mandal, A., Palva, E. T., Tunnela, O. E., & Londesborough, J. (1996) · Nature · doi:10.1038/379683a0 · Cited by 312 · see citing works
Trehalose and trehalase in plants: recent developments
Müller, J., Böller, T., & Wiemken, A. (1995) · Plant Science · doi:10.1016/0168-9452(95)04218-j · Cited by 212 · see citing works
Preservation records of micro-organisms: evidence of the tenacity of life
Kennedy, M., Reader, S. L., & Swierczynski, L. M. (1994) · Microbiology · doi:10.1099/00221287-140-10-2513 · Cited by 211 · see citing works
Is vitrification involved in depression of the phase transition temperature in dry phospholipids?
Crowe, J. H., Hoekstra, F. A., Nguyen, K. H. N., & Crowe, L. M. (1996) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(95)00287-1 · Cited by 198 · see citing works
Self-citationLipid phase transitions measured in intact cells with fourier transform infrared spectroscopy
Crowe, J. H., Hoekstra, F. A., Crowe, L. M., et al. (1989) · Cryobiology · doi:10.1016/0011-2240(89)90035-7 · Cited by 183 · see citing works
Self-citationSomero, G. N., Osmond, C. B., & Bolis, C. L. (1992) · Cited by 179 · see citing works
Staphylococcus succinus sp. nov., isolated from Dominican amber
Lambert, L., Cox, T., Mitchell, K., et al. (1998) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-48-2-511 · Cited by 117 · see citing works
Membrane Integrity in Anhydrobiotic Organisms: Toward a Mechanism for Stabilizing Dry Cells
Crowe, J. H., & Crowe, L. M. (1992) · Springer eBooks · doi:10.1007/978-3-642-76682-4_7 · Cited by 106 · see citing works
Self-citationTsvetkova, N. M., Phillips, B. L., Crowe, L. M., Crowe, J. H., & Risbud, S. H. (1998) · Biophysical Journal · doi:10.1016/s0006-3495(98)77736-7 · Cited by 103 · see citing works
Self-citationSneath, P. H. A. (1962) · Nature · doi:10.1038/195643a0 · Cited by 96 · see citing works
Lievense, L. C., & Riet, K. V. (1994) · Advances in biochemical engineering, biotechnology · doi:10.1007/bfb0008734 · Cited by 79 · see citing works
Protection of Freeze-Dried Escherichia coli by Trehalose upon Exposure to Environmental Conditions
Israeli, E., Shaffer, B. T., & Lighthart, B. (1993) · Cryobiology · doi:10.1006/cryo.1993.1052 · Cited by 67 · see citing works
Curatti, L., Folco, E. J. E., Desplats, P., et al. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.24.6776-6779.1998 · Cited by 59 · see citing works
Lievense, L. C., & Riet, K. V. ’. (1993) · Advances in biochemical engineering, biotechnology · doi:10.1007/bfb0007386 · Cited by 40 · see citing works
Coupled Enzymatic Assay for the Determination of Sucrose
HOLMES, E. W. (1997) · Analytical Biochemistry · doi:10.1006/abio.1996.9865 · Cited by 34 · see citing works
Control of matric water potential (ψm) in immobilised cultures of cyanobacteria
Potts, M., Bowman, M. A., & Morrison, N. S. (1984) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1984.tb01303.x · Cited by 24 · see citing works
Self-citationPriest, F. G. (1995) · PubMed · Cited by 17 · see citing works
Priest, F. G. (1995) · Science · doi:10.1126/science.270.5244.2015-a · Cited by 16 · see citing works
Tobacco-plant desiccation tolerance
Gaff, D. F. (1996) · Nature · doi:10.1038/382502a0 · Cited by 15 · see citing works
Trehalose Is Not Relevant for In Vivo Activity of ς S -Containing RNA Polymerase in Escherichia coli
Germer, J., Muffler, A., & Hengge‐Aronis, R. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.6.1603-1606.1998 · Cited by 12 · see citing works
Postgate, J. R., & Priest, F. G. (1995) · Microbiology · doi:10.1099/13500872-141-11-2763 · Cited by 4 · see citing works
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Research works printed but not linked: 2
- [1]Billi, D., and M. Potts. Desiccation. In K. B. Storey and J. M. Storey (ed.), Cell and molecular responses to stress, in press. Elsevier Science, Amsterdam, The Netherlands.
- [10]Gerhardt P. Survival of ancient bacteria desiccated within amber: believe it or not? ASM News. 1998;64:68–69. [Google Scholar]
Cited by
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Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering
Quintana, N., Van der Kooy, F., Van de Rhee, M. D., Voshol, G. P., & Verpoorte, R. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3394-0 · Cited by 338 · see citing works
Compatible solute biosynthesis in cyanobacteria
Klähn, S., & Hagemann, M. (2011) · Environmental Microbiology · doi:10.1111/j.1462-2920.2010.02366.x · Cited by 268 · see citing works
Origin of sucrose metabolism in higher plants: when, how and why?
Salerno, G. (2003) · Trends in Plant Science · doi:10.1016/s1360-1385(02)00029-8 · Cited by 202 · see citing works
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Self-citation
- Authors
- Billi, D., Wright, D. J., Helm, R. F., Prickett, T., Potts, M., & Crowe, J. H.
- Affiliations
- Virginia Tech (US)
- University of California, Davis (US)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000-04
- Volume
- 66
- Issue
- 4
- Pages
- 1680-1684
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC92041
- OpenAlex
- W2056031907
- Cited by
- 103
- References
- 29
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 3 · 2022: 3 · 2021: 6 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 5 · 2014: 6 · 2013: 6 · 2012: 4
- FWCI
- 3.89
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- U.S. Naval Research Laboratory
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- desiccation tolerance, Escherichia coli, SPSA, Synechocystis sp. PCC 6803, freeze-drying, air drying, phospholipid phase transition, membrane fluidity, phosphorus pentoxide, recombinant protein expression
- MeSH terms
- Cyanobacteria, Desiccation, Escherichia coli, Freeze Drying, Genetic Engineering, Glucosyltransferases, Sucrose, Transformation, Genetic, Phosphorus Compounds
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