The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Bioaugmentation of Activated Sludge by an Indigenous 3-Chloroaniline-Degrading Comamonas testosteroni Strain, I2 gfp
Open accessAbstract
A strain identified as Comamonas testosteroni I2 was isolated from activated sludge and found to be able to mineralize 3-chloroaniline (3-CA). During the mineralization, a yellow intermediate accumulated temporarily, due to the distal meta -cleavage of chlorocatechol. This strain was tested for its ability to clean wastewater containing 3-CA upon inoculation into activated sludge. To monitor its survival, the strain was chromosomally marked with the gfp gene and designated I2 gfp . After inoculation into a lab-scale semicontinuous activated-sludge (SCAS) system, the inoculated strain maintained itself in the sludge for at least 45 days and was present in the sludge flocs. After an initial adaptation period of 6 days, complete degradation of 3-CA was obtained during 2 weeks, while no degradation at all occurred in the noninoculated control reactor. Upon further operation of the SCAS system, only 50% 3-CA removal was observed. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes revealed a dynamic change in the microbial community structure of the activated sludge. The DGGE patterns of the noninoculated and the inoculated reactors evolved after 7 days to different clusters, which suggests an effect of strain inoculation on the microbial community structure. The results indicate that bioaugmentation, even with a strain originating from that ecosystem and able to effectively grow on a selective substrate, is not permanent and will probably require regular resupplementation.
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Bioaugmentation and its application in wastewater treatment: A review
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Microorganisms relevant to bioremediation
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Self-citationBoon, N., Top, E. M., Verstraete, W., & Siciliano, S. D. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.3.1511-1520.2003 · Cited by 257
Self-citationFarrell, A., & Quilty, B. (2002) · Journal of Industrial Microbiology and Biotechnology · doi:10.1038/sj.jim.7000249 · Cited by 122
Metadata
- Authors
- Boon, N., Goris, J., De Vos, P., Verstraete, W., & Top, E. M.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000-07
- Volume
- 66
- Issue
- 7
- Pages
- 2906-2913
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2142374516
- Cited by
- 351
- References
- 30
- FWCI
- 17.57
- Type
- journal article
- Language
- en
- Open access
- green
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- Boon, N., Goris, J., De Vos, P., Verstraete, W., & Top, E. M. (2000). Bioaugmentation of Activated Sludge by an Indigenous 3-Chloroaniline-Degrading Comamonas testosteroni Strain, I2 gfp. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.66.7.2906-2913.2000
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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