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New Vector for Efficient Allelic Replacement in Naturally Nontransformable, Low-GC-Content, Gram-Positive Bacteria
Open accessAbstract
A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-β- d- galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. The plasmid was used in Staphylococcus aureus , Listeria monocytogenes , and Bacillus cereus to efficiently construct mutants with or without an associated antibiotic resistance gene.
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Citations
Cited by
CRISPR RNA Maturation by Trans-Encoded Small RNA and Host Factor RNase III
Deltcheva, E., Chylinski, K., Sharma, C. M., Gonzales, K., Chao, Y., Pirzada, Z. A., Eckert, M. R., Vogel, J., & Charpentier, E. (2011) · Nature · doi:10.1038/nature09886 · Cited by 2,563
Boisset, S., Geissmann, T., Huntzinger, E., Fechter, P., Bendridi, N., Possedko, M., Chevalier, C., Helfer, A. C., Benito, Y., Jacquier, A., et al. (2007) · Genes & Development · doi:10.1101/gad.423507 · Cited by 490
Genestier, A.-L., Michallet, M.-C., Prévost, G., Bellot, G., Chalabreysse, L., Peyrol, S., Thivolet, F., Etienne, J., Lina, G., Vallette, F. M., et al. (2005) · Journal of Clinical Investigation · doi:10.1172/jci22684 · Cited by 416
Staphylococcus aureus RNAIII and the endoribonuclease III coordinately regulate spa gene expression
Huntzinger, E., Boisset, S., Saveanu, C., Benito, Y., Geissmann, T., Namane, A., Lina, G., Etienne, J., Ehresmann, B., Ehresmann, C., et al. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600572 · Cited by 361
Functional microdomains in bacterial membranes
López, D., & Kolter, R. (2010) · Genes & Development · doi:10.1101/gad.1945010 · Cited by 346
Metadata
- Authors
- Arnaud, M., Chastanet, A., & Débarbouillé, M.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-11
- Volume
- 70
- Issue
- 11
- Pages
- 6887-6891
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1978163337
- Cited by
- 944
- References
- 0
- FWCI
- 6.40
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: New Vector for Efficient Allelic Replacement in Naturally Nontransformable, Low-GC-Content, Gram-Positive Bacteria. https://app.cochat.ai/papers/10.1128/aem.70.11.6887-6891.2004
- The paper
- Arnaud, M., Chastanet, A., & Débarbouillé, M. (2004). New Vector for Efficient Allelic Replacement in Naturally Nontransformable, Low-GC-Content, Gram-Positive Bacteria. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.70.11.6887-6891.2004
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
